Abstract:
:Over 70% of head & neck squamous cell carcinoma (HNSCC) patients carry TP53 oncogenic mutations. Here we studied the role of specific tumor-derived mutant p53 proteins in the aberrant transcription of long non-coding (lnc) MIR205HG gene in head and neck cancer cells. Methods: To understand the role of lncMIR205HG, that we showed to be transcriptionally regulated by mutant p53 in HNSCC, we have employed siRNA and shRNA in CAL27 and FaDu HNSCC cell lines to suppress p53 gene expression in ChIP assays and RT-qPCR. We validated our findings in a cohort of 522 HNSCC patients from The Cancer Genome Atlas Data Portal (TCGA). We further evaluated our results in 63 HNSCC tumor samples collected at our institute, 32 of which were characterized by mutated TP53 (missense mutations) while 31 were characterized by wild-type TP53. Results: Maturation of pre-MIR205HG transcript produces two non-coding RNAs, lncMIR205HG and hsa-miR-205-5p. Down-regulation of lncMIR205HG expression significantly reduced cell proliferation, cell migration and clonogenic activity of head and neck cancer cells. Expression of MIR205HG was significantly increased in HNSCC with mutated TP53 when compared with matched non-tumoral tissues. Furthermore, MIR205HG expression levels were significantly higher in tumoral samples with mutant p53 than in tumoral tissues expressing wild-type p53. Mechanistically, MIR205HG depletes endogenous miR-590-3p leading to increased cyclin B, cdk1, and YAP protein expression. Conclusions: Taken together, these findings identify a transcriptional and post-transcriptional molecular network that includes mutant p53 protein, lncMIR205HG, YAP, and other proliferation-related genes, which are enriched in HNSCC patients with poor prognosis.
journal_name
Theranosticsjournal_title
Theranosticsauthors
Di Agostino S,Valenti F,Sacconi A,Fontemaggi G,Pallocca M,Pulito C,Ganci F,Muti P,Strano S,Blandino Gdoi
10.7150/thno.22167subject
Has Abstractpub_date
2018-02-12 00:00:00pages
1850-1868issue
7issn
1838-7640pii
thnov08p1850journal_volume
8pub_type
杂志文章相关文献
Theranostics文献大全abstract::In advanced atherosclerotic plaques, defective efferocytosis of apoptotic foam cells and decreased cholesterol efflux contribute to lesion progression. In our previous study, we demonstrated that 5-aminolevulinic acid (ALA)-mediated sonodynamic therapy (SDT) could induce foam cells apoptosis via the mitochondrial-casp...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.26193
更新日期:2018-09-29 00:00:00
abstract::To enhance effective drug accumulation in drug-resistant tumors, a site-specific drug-releasing polypeptide system (PEG-Phis/Pasp-DOX/CA4) was exploited in response to tumor extracellular and intracellular pH. This system could firstly release the embedded tumor vascular inhibitor (CA4) to transiently 'normalize' vasc...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.11821
更新日期:2015-04-28 00:00:00
abstract::Background: Tobacco smoke (TS) critically contributes to the development of lung cancer; however, the underlying molecular mechanisms remain unclear. The induction of cancer stem cells (CSCs) by TS represents an early event in tumor initiation. The lung cancer-related gene ΔNp63α is highly expressed in epithelial tiss...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.33812
更新日期:2019-07-09 00:00:00
abstract::During the last few decades, cell-based anti-tumor immunotherapy emerged and it has provided us with a large amount of knowledge. Upon chemokines recognition, immune cells undergo rapid trafficking and activation in disease milieu, with immune cells chemotaxis being accompanied by activation of diverse intercellular s...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.45219
更新日期:2020-05-15 00:00:00
abstract::Lung cancer is the leading cause of cancer-related death worldwide. Most targeted drugs approved for lung cancer treatment are tyrosine kinase inhibitors (TKIs) directed against EGFR or ALK, and are used mainly for adenocarcinoma. At present, there is no effective or tailored targeting agent for large cell carcinoma (...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.17573
更新日期:2017-04-10 00:00:00
abstract::We report a new technique to detect enzyme activity inside cells. The method based on Fluorescence Lifetime Imaging (FLIM) technology allows one to follow sensor cleavage by proteolytic enzyme caspase-3. Specifically, we use the FLIM FRET of living cells via the confocal fluorescence microscopy. A specially designed l...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.3885
更新日期:2012-01-01 00:00:00
abstract::Cancer stem cells (CSCs) are believed to be a principal cellular source for tumour progression and therapeutic drug resistance as they are capable of self-renewal and can differentiate into cancer cells. Importantly, CSCs acquire the ability to evade the killing effects of cytotoxic agents through changes at the genet...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.20725
更新日期:2017-09-13 00:00:00
abstract::Fibrinolytic therapy of venous thromboembolism (VTE) is increasingly utilized, yet limited knowledge is available regarding in vivo mechanisms that govern fibrinolytic efficacy. In particular, it is unknown how age-dependent thrombus organization limits direct blood contact with fibrin, the target of blood-based fibri...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.12494
更新日期:2015-09-13 00:00:00
abstract::Inadequate diagnostic methods for prostate cancer lead to over- and undertreatment, and the inability to intraoperatively visualize positive margins may limit the success of surgical resection. Prostate cancer visualization could be improved by combining the complementary modalities of immuno-positron emission tomogra...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.27679
更新日期:2018-11-12 00:00:00
abstract::Circulating tumor cells (CTCs) have shown promising potential as liquid biopsies that facilitate early detection, prognosis, therapeutic target selection and monitoring treatment response. CTCs in most cancer patients are low in abundance and heterogeneous in morphological and phenotypic profiles, which complicate the...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.18588
更新日期:2017-06-25 00:00:00
abstract::Emerging evidence indicates that reprogramming of energy metabolism involving disturbances in energy production from a defect in cellular respiration with a shift to glycolysis is a core hallmark of cancer. Alterations in cancer cell energy metabolism are linked to abnormalities in mitochondrial function. Mitochondria...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.33100
更新日期:2019-05-26 00:00:00
abstract::Systemic infusion of bone marrow-derived mesenchymal stem cells (BMSCs) has become a promising strategy for disease treatment and tissue regeneration. Strategies to enhance the efficiency of BMSC cell therapy are crucial to promote its clinical application. Here, we aimed to improve BMSC cell therapy by inhibiting the...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.16911
更新日期:2017-01-01 00:00:00
abstract::Rationale: The incidence of hepatocellular carcinoma is rising worldwide. It is predicted that nearly half of the early-stage hepatocellular carcinoma (E-HCC) patients will develop recurrence. Dysregulated pH, a hallmark of E-HCC, is correlated with poor prognosis. The acidic microenvironment has been shown to promote...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.30958
更新日期:2019-03-16 00:00:00
abstract::Intrinsic and acquired resistance to targeted therapies is a significant clinical problem in cancer. We previously showed that resistance to regorafenib, a multi-kinase inhibitor for treating colorectal cancer (CRC) patients, can be caused by mutations in the tumor suppressor FBW7, which block degradation of the pro-s...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45363
更新日期:2020-07-09 00:00:00
abstract::Rationale: Subjects unable to sustain β-cell compensation develop type 2 diabetes. Early growth response-1 protein (EGR-1), implicated in the regulation of cell differentiation, proliferation, and apoptosis, is induced by diverse metabolic challenges, such as glucose or other nutrients. Therefore, we hypothesized that...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.40664
更新日期:2020-03-04 00:00:00
abstract::Pretargeting is a multi-step process that first has an unlabeled bispecific antibody (bsMAb) localize within a tumor by virtue of its anti-tumor binding site(s) before administering a small, fast-clearing radiolabeled compound that then attaches to the other portion of the bsMAb. The compound's rapid clearance signifi...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.3582
更新日期:2012-01-01 00:00:00
abstract::The most frequent malignancy of the pancreas is the pancreatic ductal adenocarcinoma (PDAC). Despite many efforts PDAC has still a dismal prognosis. Biomarkers for early disease stage diagnosis as a prerequisite for a potentially curative treatment are still missing. Novel blood-based markers may help to overcome this...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.29247
更新日期:2019-02-12 00:00:00
abstract::Molecular subtyping of breast cancer is of considerable interest owing to its potential for personalized therapy and prognosis. However, current methodologies cannot be used for precise subtyping, thereby posing a challenge in clinical practice. The aim of the present study is to develop a cell-specific single-strande...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.28949
更新日期:2018-11-10 00:00:00
abstract::Both adjuvants and focal ablation can alter the local innate immune system and trigger a highly effective systemic response. Our goal is to determine the impact of these treatments on directly treated and distant disease and the mechanisms for the enhanced response obtained by combinatorial treatments. Methods: We com...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.25613
更新日期:2018-06-07 00:00:00
abstract::Extracellular vesicles (EVs), including exosomes and microvesicles, derived from bone marrow stromal cells (BMSCs) have been demonstrated as key factors in the progression and drug resistance of multiple myeloma (MM). EV uptake involves a variety of mechanisms which largely depend on the vesicle origin and recipient c...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.47996
更新日期:2021-01-01 00:00:00
abstract::Bacteria-based anticancer therapies aim to overcome the limitations of current cancer therapy by actively targeting and efficiently removing cancer. To achieve this goal, new approaches that target and maintain bacterial drugs at sufficient concentrations during the therapeutic window are essential. Here, we examined ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.16135
更新日期:2016-06-20 00:00:00
abstract::[This retracts the article DOI: 10.7150/thno.14306.]. ...
journal_title:Theranostics
pub_type: 撤回出版物
doi:10.7150/thno.52610
更新日期:2020-09-30 00:00:00
abstract::Recent studies have highlighted the biological significance of RNA N6-methyladenosine (m6A) modification in tumorigenicity and progression. However, it remains unclear whether m6A modifications also have potential roles in immune regulation and tumor microenvironment (TME) formation. Methods: In this study, we curated...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.52717
更新日期:2021-01-01 00:00:00
abstract::In the mammalian skeletal system, osteogenesis and angiogenesis are intimately linked during bone growth and regeneration in bone modeling and during bone homeostasis in bone remodeling. Recent studies have expanded our knowledge about the molecular and cellular mechanisms responsible for coupling angiogenesis and bon...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.34126
更新日期:2020-01-01 00:00:00
abstract::Rationale: Among the biothiols-related diseases, sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection and can result in severe oxidative stress and damage to multiple organs. In this study, we aimed to develop a fluorescence chemosensor that can both detect GSH an...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.22252
更新日期:2018-02-03 00:00:00
abstract::[This corrects the article DOI: 10.7150/thno.21477.]. ...
journal_title:Theranostics
pub_type: 已发布勘误
doi:10.7150/thno.56462
更新日期:2021-01-01 00:00:00
abstract::Although the prognosis of differentiated thyroid cancer (DTC) is relatively good, 30-40% of patients with distant metastases develop resistance to radioactive iodine therapy due to tumor dedifferentiation. For DTC patients harboring BRAFV600E mutation, Vemurafenib, a BRAF kinase inhibitor, has dramatically changed the...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.17322
更新日期:2017-02-23 00:00:00
abstract::Successful anticancer chemotherapy requires targeting tumors efficiently and further potential to eliminate cancer stem cell (CSC) subpopulations. Since CD44 is present on many types of CSCs, and it binds specially to hyaluronic acid (HA), we tested whether coating solid lipid nanoparticles with hyaluronan (HA-SLNs)wo...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.10804
更新日期:2015-04-05 00:00:00
abstract::Because of the unparalleled efficiency and universal utility in treating a variety of disease types, siRNA agents have evolved as the future drug-of-choice. Yet, the inability of the polyanionic siRNA macromolecules to cross the cell membrane remains as the bottleneck of possible clinical applications. With the cell p...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.19863
更新日期:2017-06-25 00:00:00
abstract::Mitochondria in cancer cells maintain a more negative membrane potential than normal cells. Mitochondria are the primary source of cellular reactive oxygen species (ROS), which are necessary for photodynamic therapy. Thus, the strategy of targeting mitochondria can maximize the photodynamic therapeutic efficiency for ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.15433
更新日期:2016-10-01 00:00:00