Abstract:
:BACKGROUND Fucoxanthin is a carotenoid present in the chloroplasts of brown seaweeds. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a cytokine that selectively induces apoptosis in many tumor cells and is an attractive candidate for antitumor therapies. MATERIAL AND METHODS After human cervical cancer cell lines HeLa, SiHa, and CaSki were treated with fucoxanthin or TRAIL. Cell viability was determined by 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2-tetrazolium 5-carboxanilide (XTT) method. Apoptosis was measured by flow cytometry (FCM). Protein expression of phosphatidylinositol 3 kinase (PI3K), protein kinase B (Akt), phosphated Akt (p-Akt), NF-κB nuclear factor-k-gene binding (NF-κB). Phosphated nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (p-IκBα), was measured by Western blot analysis. mRNA expression of Bax and Bcl2 was measured by RNA preparation and quantitative reverse transcription polymerase chain reaction (RT-PCR). RESULTS In the present study, the effectiveness in terms of apoptosis was as follows: TRAIL plus fucoxanthin>fucoxanthin>TRAIL, indicating the combination of fucoxanthin and TRAIL, produced a strong synergistic effect on apoptosis in human cervical cancer cells. Additionally, we found that upstream signaling PI3K/Akt and NF-κB pathways-mediated cell apoptosis was activated by TRAIL and suppressed by fucoxanthin. By using PI3K and NF-κB inhibitors LY49002 and PDTC, we found that fucoxanthin- or TRAIL-induced apoptosis of human cervical cancer cells was obviously down-regulated. CONCLUSIONS Taken together, these findings suggest that fucoxanthin and TRAIL increased the apoptosis in human cervical cancer cells by targeting the PI3K/Akt/NF-κB signaling pathway.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Jin Y,Qiu S,Shao N,Zheng Jdoi
10.12659/msm.905360subject
Has Abstractpub_date
2018-01-01 00:00:00pages
11-18eissn
1234-1010issn
1643-3750pii
905360journal_volume
24pub_type
杂志文章abstract::BACKGROUND This study was conducted to investigate the effects of low frequency repetitive transcranial magnetic stimulation (rTMS) of the right dorsolateral prefrontal cortex on depression and cognition in patients with traumatic brain injury. MATERIAL AND METHODS To accomplish this, 13 patients who were diagnosed wi...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,随机对照试验
doi:10.12659/MSM.911385
更新日期:2018-12-04 00:00:00
abstract::BACKGROUND The present research aimed to explore the risk factors for adverse cardiovascular events in elderly patients with acute myocardial infarction (AMI) combined with NAFLD. MATERIAL AND METHODS We included 325 AMI patients hospitalized in the Department of Cardiology. AMI patients underwent emergency thrombolys...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.922913
更新日期:2020-06-01 00:00:00
abstract::BACKGROUND The aim of this study was to investigate the correlation between indocyanine green plasma disappearance rate (ICG-PDR) and allograft function as well as postoperative complications after liver transplantation. MATERIAL AND METHODS In this prospective study, 115 cases of adult liver transplantation performed...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.907783
更新日期:2018-06-01 00:00:00
abstract::The Fig 4C and 4D are the same, but the author showed them as two parallel groups. Reference: Cardioprotective Effect of Isosorbide Dinitrate Postconditioning Against Rat Myocardial Ischemia-Reperfusion Injury In Vivo Xinwen Zhao, Mengjuan Wang, Mei Li, Na Wu, Dalin Song Med Sci Monit 2019;25: 1629-1636 10.12659/MSM.9...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,撤回出版物
doi:10.12659/MSM.919342
更新日期:2019-08-26 00:00:00
abstract::BACKGROUND The aim of this study was to discuss the possible roles and clinical value of 2 circRNAs (hsa_circ_0049224 and has_circ_0049220) in SLE patients. MATERIAL AND METHODS Reverse-transcription real-time polymerase chain reaction (RT-PCR) was conducted to detect the expressions of hsa_circ_0049224, has_circ_0049...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.906507
更新日期:2018-04-02 00:00:00
abstract:BACKGROUND:This study aimed to explore the effects of progressive resistance exercise training (PRET) on hemorheology. MATERIAL/METHODS:Exercise sessions included 1-3 sets of 8-12 repetitions at 40-60% of 1-repetition maximum (1-RM) for 3 weeks and at 75-80% of 1-RM during weeks 4-12. Red blood cell (RBC) deformabilit...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 临床试验,杂志文章
doi:10.12659/msm.882878
更新日期:2012-06-01 00:00:00
abstract::BACKGROUND Competing risk analysis determines the probability of survival and considers competing events. This retrospective study aimed to undertake a competing risk analysis of prognosis in patients with esophageal carcinoma between 2006-2015 using data from the Surveillance, Epidemiology, and End Results (SEER) dat...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.918686
更新日期:2020-01-22 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.909064
更新日期:2018-11-03 00:00:00
abstract:BACKGROUND:It is well established that there is a reduction in the skin blood flow (SBF) in response to heat with age and diabetes. While it is known that high BMI creates a stress on the cardiovascular system and increases the risk of all cause of morbidity and mortality, little is known of the effect of high BMI on S...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.883878
更新日期:2013-04-10 00:00:00
abstract:BACKGROUND:The Intek-Apollo stent (Switzerland) employs a polysulfone polymer coating which has demonstrated low interaction with blood and high thrombo-resistance. The aim of this study was to assess the clinical and angiographic outcomes after Intek-Apollo stent utilisation in a real-world setting. MATERIAL/METHODS:...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2010-10-01 00:00:00
abstract:BACKGROUND:Lipolytic enzymes, such as lipase, phospholipase A(2), lipoprotein lipase, and hormone-sensitive adipocyte lipase are probably implicated in the pathogenesis of acute pancreatitis. The increased activity of these enzymes can cause specific changes in fatty acid composition, both to free fatty acid (FFA) and ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract:BACKGROUND:Haemorrhagic hypotension is accompanied by an increase in endogenous opioid system activity, however, its significance is not fully recognised. Therefore, the role of endogenous opioid peptides in the central cardiovascular regulation in haemorrhagic shock was reviewed. CONCLUSIONS:Complex central opioid me...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,评审
doi:
更新日期:2001-05-01 00:00:00
abstract::BACKGROUND In previous studies, higher expression of PREX1 (PtdIns (3,4,5)P3-dependent Rac exchanger 1) has been detected in some subsets of breast cancer, and activation of PREX1 has been associated with tumor progression in vivo. However, an association between PREX1 and breast cancer prognosis has not been examined...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.915845
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:Component mal-positioning may contribute to a poor clinical outcome after hemiarthroplasty. Eccentric head components, recently introduced, allow posterior offset of the humeral head relative to the shaft so the purpose of this study was to determine effects of mal-aligning humeral head offset. MATERIAL/MET...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2003-08-01 00:00:00
abstract::Writing and reading 'Discussion' sections in medical articles require a procedure as exact and structured as that involved in raising questions, choosing materials and methods and producing results for a health research study. The medical article as a whole can be considered an exercise in modern argumentation and its...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00
abstract::BACKGROUND Type 2 diabetes impairs the healing process and induces apoptosis of fibroblasts, which are thought to be involved in this process. We investigated the possible mechanisms involved in AGEs-induced apoptosis of human dermal fibroblasts. MATERIAL AND METHODS We examined the expression of apoptosis-related pro...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.915806
更新日期:2019-10-06 00:00:00
abstract::BACKGROUND Traditional diagnostic methods for tuberculosis (TB) cannot be reliably applied to tuberculous pleurisy. Therefore, this prospective, randomized, controlled trial was performed to compare the diagnostic sensitivity and safety of ultrasound-guided cutting-needle pleural biopsy versus thoracoscopic pleural bi...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,随机对照试验
doi:10.12659/MSM.912506
更新日期:2018-10-10 00:00:00
abstract::BACKGROUND The objective of this study was to explore the role of SIX1 in paclitaxel (TAX) resistance of HepG2 cells via reactive oxygen species (ROS) and autophagy pathway. MATERIAL AND METHODS Hepatoma cell line HepG2 was treated with SIX1 knockdown or/and TAX. Cell growth was detected by MTT assay and colony format...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.906361
更新日期:2018-04-15 00:00:00
abstract::BACKGROUND Lower limb and pelvic injuries and fractures occur at a very high incidence in motor vehicle accidents. In this study, the characteristics (e.g., body side, bone location, and fracture severity) of lower limb and pelvic fractures that occurred during front-impact collisions were correlated with the injured ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.898459
更新日期:2016-12-01 00:00:00
abstract::Obtaining and critically appraising evidence is clearly not enough to make better decisions in clinical care. The evidence should be linked to the clinician's expertise, the patient's individual circumstances (including values and preferences), and clinical context and settings. We propose critical thinking and decisi...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,评审
doi:10.12659/msm.881321
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Dermatitis artefacta (DA) is a dermatologicopsychiatric illness that is a conscious self-infliction of lesions to accessible regions of the body. The lesions usually do not resemble those of any know skin disease and there are no specific diagnostic tests to recognize them. This makes dermatitis artefacta a ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,评审
doi:10.12659/msm.883474
更新日期:2012-10-01 00:00:00
abstract::BACKGROUND Chronic obstructive pulmonary disease (COPD) is a commonly diagnosed condition in people older than 50 years of age. In advanced stage of this disease, integrated care (IC) is recommended as an optimal approach. IC allows for holistic and patient-focused care carried out at the patient's home. The aim of th...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.901982
更新日期:2017-06-12 00:00:00
abstract::BACKGROUND Our previous study found a novel fluid combination with better resuscitation effects under hypotensive condition at the early stage of uncontrolled hemorrhagic shock (UHS). However, the optimal recovery concentration of hypertonic saline in this fluid combination remains unknown. This experiment aimed to ex...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.916937
更新日期:2019-10-30 00:00:00
abstract:BACKGROUND:Patients with their given complaints and symptoms expect certain diagnoses to be estimated with the same probability when seen by different family doctors. These estimations showed extreme variation among family doctors in Switzerland. The present study investigated whether there is any difference in the pro...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2010-03-01 00:00:00
abstract:BACKGROUND:Fractional exhaled nitric oxide (FENO) is nitric oxide (NO) in the lower airway measured by oral exhalation. FENO can be a useful non-invasive marker for asthma. Paraquat-mediated lung injury can be reflective of an ROS-induced lung injury. We aimed to verify if FENO is a clinical parameter of ROS formation ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.889820
更新日期:2014-02-01 00:00:00
abstract::Heavy metals tend to occur in increasingly many aspects of human activities. Studies of cadmium (Cd) have revealed that it is extremely toxic in its effects. It is known that selenium (Se) may suppress deleterious effects of Cd. We investigated the effects of dietary Cd-intoxication on the incorporation of precursors ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract:BACKGROUND:Haplotype analysis is hypothesized as having better power than individual SNPs in detecting the association between genotype and phenotype, but this approach has rarely been used in studies of the genetics of violent behavior in schizophrenia. The aim of this study was to further examine the role of the COMT...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2009-09-01 00:00:00
abstract::BACKGROUND During total knee arthroplasty (TKA) in varus knee deformities, reduction osteotomy (RO) and medial soft tissue release are alternative techniques to aid in achieving deformity correction. In this study, we investigated the effect of RO compared to extensive medial soft tissue release (ER) on clinical outco...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,随机对照试验
doi:10.12659/msm.905815
更新日期:2017-08-07 00:00:00
abstract::BACKGROUND Diabetic nephropathy (DN) is a potentially fatal complication of diabetes mellitus. While lifestyle changes can reduce diabetes risk, it is unclear whether improved lifestyle can slow or reverse DN progression. This study evaluated whether an intensive lifestyle intervention (IL-I) targeting weight loss and...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,随机对照试验
doi:10.12659/MSM.913512
更新日期:2019-04-25 00:00:00
abstract:BACKGROUND:To evaluate the pain, quality of life (QOL), and limb function of patients after en bloc resection of solitary metastatic bone cancer in the limbs. MATERIAL/METHODS:A total of 27 patients with solitary metastatic bone cancer in the limbs were recruited. All these patients underwent limb-salvage surgery with...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 临床试验,杂志文章
doi:10.12659/msm.883542
更新日期:2012-11-01 00:00:00