Abstract：
:Metabotropic glutamate (mGlu) receptor 5 is involved in neuroinflammation and has been shown to mediate reduced inflammation and neurotoxicity and to modify microglia polarization. On the other hand, blockade of mGlu5 receptor results in inhibition of microglia activation. To dissect this controversy, we investigated whether microvesicles (MVs) released from microglia BV2 cells could contribute to the communication between microglia and neurons and whether this interaction was modulated by mGlu5 receptor. Activation of purinergic ionotropic P2X7 receptor with the stable ATP analog benzoyl-ATP (100 μM) caused rapid MVs shedding from BV2 cells. Ionic currents through P2X7 receptor increased in BV2 cells pretreated for 24 h with the mGlu5 receptor agonist CHPG (200 μM) as by patch-clamp recording. This increase was blunted when microglia cells were activated by exposure to lipopolysaccharide (LPS; 0.1 μg/ml for 6 h). Accordingly, a greater amount of MVs formed after CHPG treatment, an effect prevented by the mGlu5 receptor antagonist MTEP (100 μM), as measured by expression of flotillin, a membrane protein enriched in MVs. Transferred MVs were internalized by SH-SY5Y neurons where they did not modify neuronal death induced by a low concentration of rotenone (0.1 μM for 24 h), but significantly increased rotenone neurotoxicity when shed from CHPG-treated BV2 cells. miR146a was increased in CHPG-treated MVs, an effect concealed in MVs from LPS-activated BV2 cells that showed per se an increase in miRNA146a levels. The present data support a role for microglia-shed MVs in mGlu5-mediated modulation of neuronal death and identify miRNAs as potential critical mediators of this interaction.
journal_name
Front Pharmacoljournal_title
Frontiers in pharmacologyauthors
Beneventano M,Spampinato SF,Merlo S,Chisari M,Platania P,Ragusa M,Purrello M,Nicoletti F,Sortino MAdoi
10.3389/fphar.2017.00812subject
Has Abstractpub_date
2017-11-09 00:00:00pages
812issn
1663-9812journal_volume
8pub_type
杂志文章abstract：:Pulmonary fibrosis is closely associated with the recruitment of fibroblasts from capillary vessels with damaged endothelial cells, the epithelial mesenchymal transition (EMT) of type II alveolar epithelial cells, and the transformation of fibroblasts to myofibroblasts. Recent studies suggest that EMT is a key factor ...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章,评审
doi：10.3389/fphar.2020.590972
更新日期：2020-12-04 00:00:00
abstract：:In glaucoma, studies revealed an involvement of the complement system. In an experimental autoimmune glaucoma model, immunization with an optic nerve homogenate antigen (ONA) led to retinal ganglion cell (RGC) loss, while intraocular pressure (IOP) remained unchanged. Here, we investigated the therapeutic effect of a ...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2019.01381
更新日期：2019-12-02 00:00:00
abstract：Background:As the original compound of many podophyllotoxin derivatives, podophyllotoxin has a beneficial antitumor effect. The mechanism of podophyllotoxin activity in triple-negative breast cancer still needs to be explored. Methods:We used cell proliferation assay, scratch and transwell experiments, and cell cycle ...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2020.01211
更新日期：2020-08-07 00:00:00
abstract：:Lipopolysaccharide (LPS) can induce bone loss by stimulating bone resorption. Natural compounds have great potential for the treatment of osteolytic bone diseases. Magnesium lithospermate B (MLB) plays an important role in protecting against oxidative damage and also has potential anti-inflammatory pharmacological pro...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2018.00064
更新日期：2018-02-06 00:00:00
abstract：:Bone defects caused by injury, disease, or congenital deformity remain a major health concern, and efficiently regenerating bone is a prominent clinical demand worldwide. However, bone regeneration is an intricate process that requires concerted participation of both cells and bioactive factors. Mimicking physiologica...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章,评审
doi：10.3389/fphar.2020.00622
更新日期：2020-05-06 00:00:00
abstract：:Trepibutone was widely used for cholelithiasis, cholecystitis, biliary tract dyskinesia, cholecystectomy syndrome, and chronic pancreatitis in clinic. However, few investigations on trepibutone have been conducted. In this study, an accurate, sensitive, and selective analytical method was developed and successfully ap...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2019.01266
更新日期：2019-11-04 00:00:00
abstract：:The immunotherapy agent pembrolizumab has been approved for gastric cancer (GC) patients with recurrent or advanced disease who are PD-L1 positive. Mutations in the primary lesion may drive the expression of immune targets thereby priming the tumor to therapeutic sensitivity. In this study, we aimed to uncover mutatio...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2018.01522
更新日期：2019-01-08 00:00:00
abstract：:Localized neuropathic pain can be relieved following the topical application of high-concentration capsaicin. This clinical effect is thought to be related to the temporary desensitization of capsaicin- and heat-sensitive epidermal nociceptors. The objective of the present study was to examine whether the changes in t...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2020.00615
更新日期：2020-05-19 00:00:00
abstract：:Cissus quadrangularis (C. quadrangularis) is a plant of the Vitaceae family known for its anticonvulsant effects in traditional medicine. The objective of this study was to elucidate the anxiolytic and antiepileptic effects of aqueous extract of C. quadrangularis. The mice were divided into different groups and treate...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2018.00751
更新日期：2018-07-17 00:00:00
abstract：PURPOSE:Colostrum has been proposed to treat severe dryness and problematic eye lesions showing a beneficial effect. The aim of the study was to investigate the effect of 2-fucosyl-lactose, a natural sugar present in the human colostrum, in an experimental dry eye. METHODS:Dry eye was induced in adult male New Zealand...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2015.00280
更新日期：2015-11-18 00:00:00
abstract：:Background: New treatment platforms that can prevent acute respiratory distress syndrome (ARDS) or reduce its mortality rate in high-risk coronavirus disease 2019 (COVID-19) patients, such as those with an underlying cancer, are urgently needed. Rejuveinix (RJX) is an intravenous formulation of anti-oxidants and anti-...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2020.594321
更新日期：2020-11-10 00:00:00
abstract：Introduction:Practical adherence barriers (e.g., medication frequency) are generally more amenable to intervention than perceptual barriers (e.g., beliefs). Measures which assess adherence barriers exist, however these tend to measure a mix of factors. There is a need to identify what practical barriers are captured by...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章,评审
doi：10.3389/fphar.2020.00572
更新日期：2020-05-13 00:00:00
abstract：:Cytochrome P450 3A4 (CYP3A4) enzyme activity is known to show considerable ethnic heterogeneity and inter-individual differences, affecting the outcome of drug treatment. CYP3A4 genetic polymorphisms are believed to be one of the important causes, leading to inter-individual variability in drug metabolism. Quinine is ...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2019.00591
更新日期：2019-05-31 00:00:00
abstract：:Aseptic loosening induced by periprosthetic osteolysis (PPO) is the leading complication of total joint arthroplasty (TJA) and results in patients having to receive revision surgery. However, there is still no efficient drug to prevent or even slow the pathological process. Herein, we report novel dual-targeted, curcu...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2020.600156
更新日期：2020-12-04 00:00:00
abstract：:Cerebral energy deficiency is a key pathophysiologic cascade that results in neuronal injury and necrosis after ischemic stroke. Shengui Sansheng San (SSS) has been used to treat stroke for more than 300 years. In present study, we investigated the therapeutic efficacy and mechanism of SSS extraction on cerebral energ...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2019.00386
更新日期：2019-04-23 00:00:00
abstract：:Impaired apoptotic clearance of myofibroblasts can result in the continuous expansion of scar tissue during the persistent injury in the lung. However, the molecular and cellular mechanisms underlying the apoptotic clearance of multiple mesenchymal cells including fibrocytes, fibroblasts and myofibroblasts in severe f...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2019.00532
更新日期：2019-05-17 00:00:00
abstract：:Disruptions in iron homeostasis are linked to a broad spectrum of chronic conditions including cardiovascular, malignant, metabolic, and neurodegenerative disease. Evidence supporting this contention derives from a variety of analytical approaches, ranging from molecular to population-based studies. This review focuse...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章,评审
doi：10.3389/fphar.2014.00117
更新日期：2014-05-20 00:00:00
abstract：INTRODUCTION:Reliable and precise measurement of patient adherence to medications is feasible by incorporating a microcircuitry into pharmaceutical packages of various designs, such that the maneuvers needed to remove a dose of drug are detected, time-stamped, and stored. The principle is called "electronic medication ...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2013.00026
更新日期：2013-03-13 00:00:00
abstract：:Objective: The purpose of this study was to evaluate the anticancer effects of Ophiopogonin D' (OPD', a natural product extracted from a traditional Chinese medicine (Radix Ophiopogonis) against androgen-independent prostate cancer cells and to explore the underlying molecular mechanism(s) of action. Methods: The CCK-...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2018.00432
更新日期：2018-04-30 00:00:00
abstract：:In metastatic renal cell carcinoma, complete response to first-line antiangiogenic agents is rare and resistance to therapy often develops. Protocols for sequential treatment with angiogenesis and mTOR inhibitors are under evaluation to improve outcomes. In this observational, real-world study, patients received a fir...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2017.00484
更新日期：2017-07-20 00:00:00
abstract：:We aimed to ascertain the mechanism underlying the effects of acteoside (ACT) from Ligustrum robustum (Roxb.) Blume (Oleaceae) on lipid metabolism and synthesis. ACT, a water-soluble phenylpropanoid glycoside, is the most abundant and major active component of L. robustum; the leaves of L. robustum, known as kudingcha...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2019.00602
更新日期：2019-05-24 00:00:00
abstract：:The potential of multifunctional wound heal biomaterial relies on the optimal content of therapeutic constituents as well as the desirable physical, chemical, and biological properties to accelerate the healing process. Formulating biomaterials such as amnion or collagen based scaffolds with natural products offer an ...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2017.00433
更新日期：2017-07-18 00:00:00
abstract：:Cervical cancer is the fourth most common cancer that affects women, mainly through human papilloma virus (HPV) infection with high-risk HPV16 and HPV18. The present study investigated the in vitro anticancer activity and mechanism of action of a proanthocyanidin polymer-rich fraction of Stryphnodendron adstringens (F...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2018.00694
更新日期：2018-07-03 00:00:00
abstract：:Flavonoids is an intensively studied group of natural compounds with antioxidant, antineoplastic, antihyperglycemic, cardioprotective, and neuroprotective properties. The present study intends to investigate the geroprotective action of three selected flavonoids (naringin, luteolin, chrysin) in two model organisms, Ca...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2017.00884
更新日期：2017-12-07 00:00:00
abstract：:In recent years, the heme catabolic pathway is considered to play an important regulatory role in cell protection, apoptosis, inflammation, and other physiological and pathological processes. An appropriate amount of heme forms the basic elements of various life activities, while when released in large quantities, it ...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章,评审
doi：10.3389/fphar.2019.00825
更新日期：2019-07-24 00:00:00
abstract：:Using pharmacogenetics in guiding drug therapy experiences a steady increase in uptake, although still leads to discussions as to its clinical use. Psychiatry constitutes a field where pharmacogenomic testing might help in guiding drug therapy. To address current challenges, this minireview provides an update regardin...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章,评审
doi：10.3389/fphar.2020.575540
更新日期：2020-09-11 00:00:00
abstract：:Background and Purpose:Drynaria fortunei J. Sm (D. fortunei), known as Gu-Sui-Bu, is used in traditional Chinese medicine to treat common injuries, including bone fractures and bruising. The specific functional mechanisms of the angiogenic and endothelial cell migration properties of D. fortunei are currently unclear....
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2018.00979
更新日期：2018-09-21 00:00:00
abstract：:In studies comparing regular versus on-demand treatment for patients with mild persistent asthma, on-demand treatment seems to have a similar efficacy on clinical and functional outcomes, but it does not suppress chronic airway inflammation or airway hyper-responsiveness (AHR) associated with asthma. Data on the effic...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2011.00035
更新日期：2011-07-14 00:00:00
abstract：:The objective of this study was to test the hypothesis that serotonin O-sulphate (5-HT-SO4) could be quantified in human plasma using modern liquid chromatography-mass spectrometry (LC-MS) method as well as develop and validate that method. First, a suitable LC-MS method for detection of 5-HT-SO4 in human plasma sampl...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2014.00062
更新日期：2014-04-08 00:00:00
abstract：:Uncontrolled bacteremia is a common and life threatening condition that can lead to sepsis and septic shock with significant morbidity and mortality. Neutrophil granulocytes, the most abundant phagocytic leukocyte of the innate immune system, play an essential role in capturing and killing invading pathogens. Their an...
journal_title：Frontiers in pharmacology
pub_type： 杂志文章
doi：10.3389/fphar.2019.00323
更新日期：2019-04-05 00:00:00
