Abstract:
:The therapy of breast cancer is encumbered by drug resistance and metastasis, which can be due to a defective PI3K/AKT/mTOR signaling pathway. This study was aimed at improving the anti-cancer effect of the chemotherapeutic agent paclitaxel (PTX) on the drug resistant and metastatic breast cancer by co-delivering PTX and a siRNA, siAkt, directed at silencing the Akt expression. Methods: The pH-sensitive amphiphilic polymer, poly [(1,4-butanediol)-diacrylate-β-N, N-diisopropylethylenediamine]-polyethyleneimine (BDP) was synthesized. The PTX-loaded BDP micelle/siAkt nano-complex (PMA) was prepared and characterized. The cellular uptake, cytotoxicity, RNA interference efficiency, biodistribution, pharmacokinetics, pharmacodynamics, and biocompatibility of PMA in the murine metastatic breast cancer 4T1 cells and the 4T1 tumor-bearing mice were evaluated. Results: PMA was stable at the neutral as well as tumor extracellular pH and released the drugs in the intra-endo/lysosome acidic environment. In 4T1 cells, the RNA interference against the Akt gene down-regulated the expression of Akt and P-glycoprotein and up-regulated the expression of Caspase-3. The down-regulated P-gp inhibits the efflux of PTX thereby increasing its intracellular concentration, improving the cytotoxicity, and inhibiting the migration and invasion of 4T1 cells. In the 4T1 tumor-bearing mice, co-delivery of PTX and siAkt by PMA achieved a tumor inhibiting rate of 94.1% and suppressed 96.8% lung metastases. PMA did not cause pathological lesions in normal organs. Conclusion: PMA, by virtue of overcoming drug resistance and simultaneously restraining lung metastasis, might be an efficient drug delivery system for the therapy of breast cancer.
journal_name
Theranosticsjournal_title
Theranosticsauthors
Yin J,Lang T,Cun D,Zheng Z,Huang Y,Yin Q,Yu H,Li Ydoi
10.7150/thno.21516subject
Has Abstractpub_date
2017-09-26 00:00:00pages
4204-4216issue
17issn
1838-7640pii
thnov07p4204journal_volume
7pub_type
杂志文章相关文献
Theranostics文献大全abstract::Rationale: Within the field of personalized medicine there is an increasing focus on designing flexible, multifunctional drug delivery systems that combine high efficacy with minimal side effects, by tailoring treatment to the individual. Methods: We synthesized a chemically stabilized ~4 nm nucleic acid nanoscaffold,...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.32060
更新日期:2019-04-13 00:00:00
abstract::Objective: Vascular smooth muscle cells (VSMCs) undergo the phenotypic changes from contractile to synthetic state during vascular remodeling after ischemia. SIRT1 protects against stress-induced vascular remodeling via maintaining VSMC differentiated phenotype. However, the effect of smooth muscle SIRT1 on the functi...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.39320
更新日期:2020-01-01 00:00:00
abstract::Background: Lysophosphatidic acid (LPA) is a small glycerophospholipid that acts as a potent extracellular signal in various biological processes and diseases. Our previous work demonstrated that the expression of the LPA receptors LPA1 and LPA3 is elevated in the early postnatal heart. However, the role of this stage...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.47913
更新日期:2020-08-29 00:00:00
abstract::The development of nanomedicine is expected to provide an innovative direction for addressing challenges associated with multidrug-resistant (MDR) bacteria. In the past decades, although nanotechnology-based phototherapy has been developed for antimicrobial treatment since it rarely causes bacterial resistance, the cl...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.52729
更新日期:2020-10-30 00:00:00
abstract::Proper selection and effective delivery of combination drugs targeting multiple pathophysiological pathways key to spinal cord injury (SCI) hold promise to address the thus far scarce clinical therapeutics for improving recovery after SCI. In this study, we aim to develop a clinically feasible way for targeted deliver...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.26717
更新日期:2018-08-07 00:00:00
abstract::Cell-derived microparticles (MPs) are nano-sized vesicles released by activated cells in the extracellular milieu. They act as vectors of biological activity by carrying membrane-anchored and cytoplasmic constituents of the parental cells. Although detection and characterization of cell-derived MPs may be of high diag...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.13757
更新日期:2016-03-02 00:00:00
abstract::Mitochondria in cancer cells maintain a more negative membrane potential than normal cells. Mitochondria are the primary source of cellular reactive oxygen species (ROS), which are necessary for photodynamic therapy. Thus, the strategy of targeting mitochondria can maximize the photodynamic therapeutic efficiency for ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.15433
更新日期:2016-10-01 00:00:00
abstract::Angiogenesis enhances cancer metastasis and progression, however, the roles of transcription regulation in angiogenesis are not fully defined. ZNF322A is an oncogenic zinc-finger transcription factor. Here, we demonstrate a new mechanism of Kras mutation-driven ZNF322A transcriptional activation and elucidate the inte...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.47491
更新日期:2020-08-08 00:00:00
abstract::Producing keratinocyte cells (KCs) in large scale is difficult due to their slow proliferation, disabling their use as seed cells for skin regeneration and wound healing. Cell reprogramming is a promising inducer-based approach to KC production but only reaches very low cellular conversion. Here we reported a unique c...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.31526
更新日期:2019-07-09 00:00:00
abstract::Meniscus deficiency, the most common and refractory disease in human knee joints, often progresses to osteoarthritis (OA) due to abnormal biomechanical distribution and articular cartilage abrasion. However, due to its anisotropic spatial architecture, complex biomechanical microenvironment, and limited vascularity, m...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44270
更新日期:2020-04-06 00:00:00
abstract::Peptide-derived nanocomposites have been exhibiting fascinating biological advantages, including but not limited to excellent biocompatibility, biological degradation, high targetability and subsequent potent therapeutic efficacy. While some successes have been achieved in the nanoengineering of peptide-based architec...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.47243
更新日期:2020-07-09 00:00:00
abstract::Aims: Pathological cardiac fibrosis and hypertrophy are common features of left ventricular remodeling that often progress to heart failure (HF). Endothelial cells (ECs) are the most abundant non-myocyte cells in adult mouse heart. Simvastatin, a strong inducer of Krüppel-like Factor 2 (Klf2) in ECs, ameliorates press...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.48153
更新日期:2021-01-01 00:00:00
abstract::RNA molecules (e.g., siRNA, microRNA, and mRNA) have shown tremendous potential for immunomodulation and cancer immunotherapy. They can activate both innate and adaptive immune system responses by silencing or upregulating immune-relevant genes. In addition, mRNA-based vaccines have recently been actively pursued and ...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.35568
更新日期:2020-01-01 00:00:00
abstract::Rationale: Emerging evidence suggests that noncentrosomal microtubules play an essential role in intracellular transport, cell polarity and cell motility. Whether these noncentrosomal microtubules exist or function in cancer cells remains unclear. Methods: The expression and prognostic values of CAMSAP2 and its functi...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.42596
更新日期:2020-02-19 00:00:00
abstract::The relatively new field of nanotheranostics combines the advantages of in vivo diagnosis with the ability to administer treatment through a single nano-sized carrier, offering new opportunities for cancer diagnosis and therapy. Nanotheranostics has facilitated the development of nanomedicine through direct visualizat...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.4381
更新日期:2012-01-01 00:00:00
abstract::Dysregulation of microRNAs (miRNAs) is involved in the initiation and progression of several human cancers, including breast cancer (BC), as strong evidence has been found that miRNAs can act as oncogenes or tumor suppressor genes. This review presents the state of the art on the role of miRNAs in the diagnosis, progn...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.11543
更新日期:2015-07-13 00:00:00
abstract::This paper reports a sensitive method with electrochemical technique to detect various proteases, which can be used for the diagnosis of prostate cancer. For the proposed assay method, the working electrode is modified with the peptide probes for the target proteases. These probes contain the substrate sequence of tar...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.8803
更新日期:2014-04-25 00:00:00
abstract::Development of efficient therapeutic strategy to incorporate ultrasound (US)-triggered sonodynamic therapy (SDT) and ferroptosis is highly promising in cancer therapy. However, the SDT efficacy is severely limited by the hypoxia and high glutathione (GSH) in the tumor microenvironment, and ferroptosis is highly associ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45511
更新日期:2021-01-01 00:00:00
abstract::Granzyme B (GrB) is an essential cytotoxic effector in cancer immunotherapy as it can be a potential biomarker to predict the efficacy of immunotherapies including checkpoint inhibitors. Monitoring the Granzyme B activity in cells would help determine a patient's clinical response to treatment and lead to better treat...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.37728
更新日期:2020-01-01 00:00:00
abstract::Iron oxide nanoparticles are a useful diagnostic contrast agent and have great potential for therapeutic applications. Multiple emerging diagnostic and therapeutic applications and the numerous versatile parameters of the nanoparticle platform require a robust biological model for characterization and assessment. Here...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.3568
更新日期:2012-01-01 00:00:00
abstract::Objective: Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are essential for vascular remodeling. Natural compounds with diterpene chinone or phenolic acid structure from Salvia miltiorrhiza, an eminent medicinal herb widely used to treat cardiovascular diseases in China, can effectively a...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.46911
更新日期:2020-06-19 00:00:00
abstract::Marfan syndrome (MFS) is a rare genetic disease characterized by a matrix metalloproteases (MMPs) dysregulation that leads to extracellular matrix degradation. Consequently, MFS patients are prone to develop progressive thoracic aortic enlargement and detrimental aneurysm. Since MMPs are activated by the extracellular...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.30714
更新日期:2019-04-12 00:00:00
abstract::Salivary adenoid cystic carcinoma (SACC) is a peculiar malignant tumor, characterized by its slow but inexorable growth, with a high incidence of lung metastasis and poor prognosis. Here, we show the upregulated expression of EGFR ligand epiregulin in a subset of SACC cells correlates with lung metastasis and unfavora...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.19712
更新日期:2017-08-23 00:00:00
abstract::Recently, rational design of a new class of contrast agents (CAs), based on biopolymers (hydrogels), have received considerable attention in Magnetic Resonance Imaging (MRI) diagnostic field. Several strategies have been adopted to improve relaxivity without chemical modification of the commercial CAs, however, unders...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.27313
更新日期:2019-02-28 00:00:00
abstract::Gastroenteropancreatic neuroendocrine neoplasms are heterogeneous in their clinical behavior and require therapies specially tailored according to staging, grading, origin and expression of peptide receptors. Despite extensive scientific efforts, the therapy options are still not satisfactory. The main reasons are due...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.7851
更新日期:2014-01-29 00:00:00
abstract::Rationale: P21-activated kinase 6 (PAK6) is a member of the class II PAKs family, which is a conserved family of serine/threonine kinases. Although the effects of PAK6 on many malignancies, especially in prostate cancer, have been studied for a long time, the role of PAK6 in mitochondria remains unknown. Methods: The ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.42874
更新日期:2020-02-03 00:00:00
abstract::Rationale: Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) is a co-inhibitory checkpoint receptor that is expressed by naïve T-cells in lymph nodes (LNs) to inhibit activation against "self" antigens (Ags). In cancer, anti-CTLA-4 blocks inhibitory action, enabling robust activation of T-cells against tumor Ags pr...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.35280
更新日期:2019-10-22 00:00:00
abstract::Great progress has been made in the field of tumor immunotherapy in the past decade. However, the therapeutic effects of immune checkpoint blockade (ICB) against ovarian cancer are still limited. Recently, an inhibitor of cyclin-dependent kinases 4 and 6 (CDK4/6i) has been reported to enhance antitumor immunity in pre...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44871
更新日期:2020-08-25 00:00:00
abstract::Background: Accumulating evidences indicate that nanomedicines greatly decrease the side effects and enhance the efficacy of colorectal cancer (CRC) treatment. In particular, the use of rectal delivery of nanomedicines, with advantages such as fast therapeutic effects and a diminishing hepatic first-pass effect, is cu...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.40318
更新日期:2020-02-18 00:00:00
abstract::Fast clearance, metabolism and systemic toxicity are major limits for the clinical use of anti-cancer drugs. Histone deacetylase inhibitors (HDACi) present these defects despite displaying promising anti-tumor properties on tumor cells in vitro and in in vivo model of cancers. Specific delivery of anti-cancer drugs in...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.13725
更新日期:2016-03-25 00:00:00