Abstract:
PURPOSE:This study aimed to determine the function of miR-15a in HCC, and identify cMyb as a target of miR-15a. METHODS:RNA expression was evaluated by quantitative real-time PCR (qRT-PCR). The effects of miR-15a or cMyb on HCC cells were evaluated by transwell migration assay and western blot analysis. CMyb, the predicted target, has been frequently verified by luciferase assay. RESULTS:MiR-15a was markedly downregulated in sphere culture HCC cells by qRT-PCR. CMyb was predicted to be a potential target of miR-15a using bioinformatics analysis. This prediction has been frequently verified by luciferase assay and western blot. A positive correlation between cMyb and the migration ability of HCC cells was demonstrated by transwell assays. MiR-15a mimic suppressed cMyb expression to weaken HCC cell migration ability. On the other hand, miR-15a inhibitor upregulated cMyb and induced HCC cell migration. CONCLUSION:MiR-15a could suppress HCC progression through the repression of cMyb, making miR-15a a potential therapeutic target.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Liu B,Sun T,Wu G,Shang-Guan H,Jiang ZJ,Zhang JR,Zheng YFsubject
Has Abstractpub_date
2017-02-15 00:00:00pages
520-532issue
2issn
1943-8141journal_volume
9pub_type
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-04-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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abstract::Enoxaparin is used postoperatively for the prevention of venous thromboembolism. In vitro studies and clinical trials have demonstrated the anticoagulant and antithrombotic efficacy of enoxaparin. In this study, we visualised thromboprophylactic and thrombolytic efficacy of enoxaparin in a laser-induced thrombus forma...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2015-01-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2015-11-15 00:00:00
abstract:OBJECTIVE:To investigate the effects of paeoniflorin, the main monomer component of Jinxueyuan granules, on the Ca2+ concentrations in salivary gland cells to further explore the salivation-promoting mechanism and effective monomer components of Jinxueyuan granules. METHODS:The salivary gland cells of suckling rats we...
journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2016-09-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-08-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
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journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
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journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::Human noroviruses (HuNoVs) are a major cause of epidemic and sporadic cases of acute gastroenteritis worldwide. Recently, human intestinal enteroids (HIEs) have been shown to support the replication of HuNoVs, and be an excellent model to study HuNoV-host interactions. We implemented the HIE system in our laboratory a...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-06-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-03-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2013-12-01 00:00:00
abstract::Wound repair is an intractable problem in clinic, with limited treatment options. Previous studies have demonstrated the therapeutic potential of Nogo-B in tissue repairs. However, the therapeutic effect of Nogo-B in HUVEC is still unknown. In this study, we examined the benefit of genetically modified Human Umbilical...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-03-15 00:00:00
abstract::Due to practical and ethical concerns associated with human experimentation, animal models have been essential in cancer research. However, the average rate of successful translation from animal models to clinical cancer trials is less than 8%. Animal models are limited in their ability to mimic the extremely complex ...
journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2014-01-15 00:00:00
abstract::Gonadal hormones have been well-known to affect brain regions known to be involved in the modulation of mood and affective-related behavior. Prenatal stress might alter hypothalamic-pituitary-gonadal axis, it could be a target for development of affective-related disorders in male offspring. The present study was desi...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-03-15 00:00:00
abstract:BACKGROUND:Chondrogenic differentiation of mesenchymal stem cells (MSCs) is important for osteoarthritis (OA) treatment. However, the specific mechanisms involved are undefined. MicroRNAs (miRNAs) downregulate protein synthesis by binding to the 3'UTR of target mRNA. METHODS:Bone marrow aspirates were obtained from OA...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-01-15 00:00:00
abstract::Postoperative fever is prevalent in many breast cancer patients. Some retrospective studies proposed that postoperative fever might also be considered as a rapid rough indicator for the poor prognosis of breast cancer patients. This study aims to explore the possible molecular mechanisms underlying the relapse of brea...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-10-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-12-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-12-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-07-15 00:00:00
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journal_title:American journal of translational research
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doi:
更新日期:2020-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2014-10-11 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-10-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-12-15 00:00:00
abstract:BACKGROUND:Single-cell RNA sequencing (scRNA-seq) was recently adopted for exploring molecular programmes and lineage progression patterns of pathogenesis of important diseases. In this study, scRNA-seq was used to identify potential markers for chondrocytes in osteoarthritis (OA) and to explore the function of differe...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-11-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2015-11-15 00:00:00