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Isolation of autologous adipose tissue-derived mesenchymal stem cells for bone repair.

Abstract：

INTRODUCTION:Adipose tissue represents an abundant and accessible source of adult stem cells that can differentiate into cells and tissues of mesodermal origin, including osteogenic cells. METHODS:This paper describes the procedure to obtain a 5-cm3 saline sample, containing the adipose-derived stem cells (ASCs) pellet, starting from lipoaspirate obtained from a conventional abdominal liposuction. RESULTS:A mean of 2.5×106 cells is isolated for each procedure; 35% (875000) of these are CD34+/CD45- cells, which express a subset of both positive (CD10, CD13, CD44, CD59, CD73, CD90, HLAABC) and negative (CD33, CD39, CD102, CD106, CD146, HLADR) cell-associated surface antigens, characterizing them as ASCs. CONCLUSIONS:This procedure is easy, effective, economic and safe. It allows the harvesting of a significant number of ASCs that are ready for one-step bony regenerative surgical procedures.

journal_name

Orthop Traumatol Surg Res

journal_title

Orthopaedics & traumatology, surgery & research : OTSR

authors

Raposio E,Bonomini S,Calderazzi F

doi

10.1016/j.otsr.2016.07.006

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

909-912

issue

7

issn

1877-0568

pii

S1877-0568(16)30100-1

journal_volume

102

pub_type

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