Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion.

Abstract:

:Host and parasite gene expression in skin biopsies from Leishmania braziliensis-infected patients were simultaneously analyzed using high throughput RNA-sequencing. Biopsies were taken from 8 patients with early cutaneous leishmaniasis and 17 patients with late cutaneous leishmaniasis. Although parasite DNA was found in all patient lesions at the time of biopsy, the patients could be stratified into two groups: one lacking detectable parasite transcripts (PTNeg) in lesions, and another in which parasite transcripts were readily detected (PTPos). These groups exhibited substantial differences in host responses to infection. PTPos biopsies contained an unexpected increase in B lymphocyte-specific and immunoglobulin transcripts in the lesions, and an upregulation of immune inhibitory molecules. Biopsies without detectable parasite transcripts showed decreased evidence for B cell activation, but increased expression of antimicrobial genes and genes encoding skin barrier functions. The composition and abundance of L. braziliensis transcripts in PTPos lesions were surprisingly conserved among all six patients, with minimal meaningful differences between lesions from patients with early and late cutaneous leishmaniasis. The most abundant parasite transcripts expressed in lesions were distinct from transcripts expressed in vitro in human macrophage cultures infected with L. amazonensis or L. major. Therefore in vitro gene expression in macrophage monolayers may not be a strong predictor of gene expression in lesions. Some of the most highly expressed in vivo transcripts encoded amastin-like proteins, hypothetical genes, putative parasite virulence factors, as well as histones and tubulin. In summary, RNA sequencing allowed us to simultaneously analyze human and L. braziliensis transcriptomes in lesions of infected patients, and identify unexpected differences in host immune responses which correlated with active transcription of parasite genes.

journal_name

PLoS Negl Trop Dis

authors

Christensen SM,Dillon LA,Carvalho LP,Passos S,Novais FO,Hughitt VK,Beiting DP,Carvalho EM,Scott P,El-Sayed NM,Mosser DM

doi

10.1371/journal.pntd.0004992

subject

Has Abstract

pub_date

2016-09-15 00:00:00

pages

e0004992

issue

9

eissn

1935-2727

issn

1935-2735

pii

PNTD-D-16-00545

journal_volume

10

pub_type

杂志文章
  • Proteomic selection of immunodiagnostic antigens for human African trypanosomiasis and generation of a prototype lateral flow immunodiagnostic device.

    abstract:BACKGROUND:The diagnosis of Human African Trypanosomiasis relies mainly on the Card Agglutination Test for Trypanosomiasis (CATT). While this test is successful, it is acknowledged that there may be room for improvement. Our aim was to develop a prototype lateral flow test based on the detection of antibodies to trypan...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0002087

    authors: Sullivan L,Wall SJ,Carrington M,Ferguson MA

    更新日期:2013-01-01 00:00:00

  • Mapping dengue risk in Singapore using Random Forest.

    abstract:BACKGROUND:Singapore experiences endemic dengue, with 2013 being the largest outbreak year known to date, culminating in 22,170 cases. Given the limited resources available, and that vector control is the key approach for prevention in Singapore, it is important that public health professionals know where resources sho...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006587

    authors: Ong J,Liu X,Rajarethinam J,Kok SY,Liang S,Tang CS,Cook AR,Ng LC,Yap G

    更新日期:2018-06-18 00:00:00

  • Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq.

    abstract:BACKGROUND:Filarial nematodes currently infect up to 54 million people worldwide, with millions more at risk for infection, representing the leading cause of disability in the developing world. Brugia malayi is one of the causative agents of lymphatic filariasis and remains the only human filarial parasite that can be ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0005357

    authors: Grote A,Voronin D,Ding T,Twaddle A,Unnasch TR,Lustigman S,Ghedin E

    更新日期:2017-03-30 00:00:00

  • Pre-operative trichiatic eyelash pattern predicts post-operative trachomatous trichiasis.

    abstract:IMPORTANCE:Trichiasis surgery programs globally have faced high rates of poor surgical outcomes. Identifying correctable risk factors for improving long-term outcomes is essential for countries targeting elimination of trachoma as a public health problem. OBJECTIVE:To determine whether the location of trichiatic eyela...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,随机对照试验

    doi:10.1371/journal.pntd.0007637

    authors: Gower EW,Munoz B,Rajak S,Habtamu E,West SK,Merbs SL,Harding JC,Alemayehu W,Callahan EK,Emerson PM,Gebre T,Burton MJ

    更新日期:2019-10-07 00:00:00

  • Killing of diverse eye pathogens (Acanthamoeba spp., Fusarium solani, and Chlamydia trachomatis) with alcohols.

    abstract:BACKGROUND:Blindness is caused by eye pathogens that include a free-living protist (Acanthamoeba castellanii, A. byersi, and/or other Acanthamoeba spp.), a fungus (Fusarium solani), and a bacterium (Chlamydia trachomatis). Hand-eye contact is likely a contributor to the spread of these pathogens, and so hand washing wi...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0005382

    authors: Aqeel Y,Rodriguez R,Chatterjee A,Ingalls RR,Samuelson J

    更新日期:2017-02-09 00:00:00

  • LABCG2, a new ABC transporter implicated in phosphatidylserine exposure, is involved in the infectivity and pathogenicity of Leishmania.

    abstract::Leishmaniasis is a neglected disease produced by the intracellular protozoan parasite Leishmania. In the present study, we show that LABCG2, a new ATP-binding cassette half-transporter (ABCG subfamily) from Leishmania, is involved in parasite virulence. Down-regulation of LABCG2 function upon expression of an inactive...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0002179

    authors: Campos-Salinas J,León-Guerrero D,González-Rey E,Delgado M,Castanys S,Pérez-Victoria JM,Gamarro F

    更新日期:2013-04-25 00:00:00

  • Clinical, environmental, and behavioral characteristics associated with Cryptosporidium infection among children with moderate-to-severe diarrhea in rural western Kenya, 2008-2012: The Global Enteric Multicenter Study (GEMS).

    abstract:BACKGROUND:Cryptosporidium is a leading cause of moderate-to-severe diarrhea (MSD) in young children in Africa. We examined factors associated with Cryptosporidium infection in MSD cases enrolled at the rural western Kenya Global Enteric Multicenter Study (GEMS) site from 2008-2012. METHODOLOGY/PRINCIPAL FINDINGS:At h...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,多中心研究

    doi:10.1371/journal.pntd.0006640

    authors: Delahoy MJ,Omore R,Ayers TL,Schilling KA,Blackstock AJ,Ochieng JB,Moke F,Jaron P,Awuor A,Okonji C,Juma J,Farag TH,Nasrin D,Panchalingam S,Nataro JP,Kotloff KL,Levine MM,Oundo J,Roellig DM,Xiao L,Parsons MB,Laser

    更新日期:2018-07-12 00:00:00

  • Correction: Female genital schistosomiasis and HIV/AIDS: Reversing the neglect of girls and women.

    abstract::[This corrects the article DOI: 10.1371/journal.pntd.0007025.]. ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 已发布勘误

    doi:10.1371/journal.pntd.0008725

    authors: Hotez PJ,Harrison W,Fenwick A,Bustinduy AL,Ducker C,Mbabazi PS,Engels D,Kjetland EF

    更新日期:2020-09-01 00:00:00

  • Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis.

    abstract:BACKGROUND:Diagnosis of urogenital schistosomiasis in chronically infected adults is challenging but important, especially because long term infection of the bladder and urinary tract can have dire consequences. We evaluated three tests for viable infection: detection of parasite specific DNA Dra1 fragments, haematuria...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0001464

    authors: Ibironke O,Koukounari A,Asaolu S,Moustaki I,Shiff C

    更新日期:2012-01-01 00:00:00

  • Emergence of melioidosis in the Indian Ocean region: Two new cases and a literature review.

    abstract::Melioidosis is a disease caused by bacteria called B. pseudomallei. Infections can develop after contact with standing water. This disease can reach all the organs and especially the lungs. It is associated with a high mortality rate (up to 50%). Melioidosis is endemic in northern Australia and in Southeast Asia. Neve...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审

    doi:10.1371/journal.pntd.0006018

    authors: Allou N,Martinet O,Allyn J,Bouchet B,Jaffar-Bandjee MC,Galas T,Traversier N,Belmonte O

    更新日期:2017-12-14 00:00:00

  • Diarrheagenic Escherichia coli carrying supplementary virulence genes are an important cause of moderate to severe diarrhoeal disease in Mexico.

    abstract::Diarrheagenic Escherichia coli (DEC) cause acute and persistent diarrhoea worldwide, but little is known about their epidemiology in Mexico. We determined the prevalence of bacterial enteropathogens in 831 children with acute diarrhoea over a four-year period in Yucatan, Mexico. Six DEC supplementary virulence genes (...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0003510

    authors: Patzi-Vargas S,Zaidi MB,Perez-Martinez I,León-Cen M,Michel-Ayala A,Chaussabel D,Estrada-Garcia T

    更新日期:2015-03-04 00:00:00

  • Animal reservoir, natural and socioeconomic variations and the transmission of hemorrhagic fever with renal syndrome in Chenzhou, China, 2006-2010.

    abstract:BACKGROUND:China has the highest incidence of hemorrhagic fever with renal syndrome (HFRS) worldwide. Reported cases account for 90% of the total number of global cases. By 2010, approximately 1.4 million HFRS cases had been reported in China. This study aimed to explore the effect of the rodent reservoir, and natural ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0002615

    authors: Xiao H,Tian HY,Gao LD,Liu HN,Duan LS,Basta N,Cazelles B,Li XJ,Lin XL,Wu HW,Chen BY,Yang HS,Xu B,Grenfell B

    更新日期:2014-01-09 00:00:00

  • Access to Chagas disease treatment in the United States after the regulatory approval of benznidazole.

    abstract::Approximately 300,000 persons in the United States (US) are infected with Trypanosoma cruzi, the protozoan that causes Chagas disease, but less than 1% are estimated to have received antiparasitic treatment. Benznidazole was approved by the US Food and Drug Administration (FDA) for treatment of T. cruzi infection in 2...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0008398

    authors: Yoshioka K,Manne-Goehler J,Maguire JH,Reich MR

    更新日期:2020-06-22 00:00:00

  • Detection and distribution of Sca autotransporter protein antigens in diverse isolates of Orientia tsutsugamushi.

    abstract::Orientia tsutsugamushi (Ots) frequently causes severe scrub typhus infections in the Asia-Pacific region. Korean investigators have demonstrated that Ots encodes five different autotransporter domain (ATD) proteins (ScaA-ScaE). ScaA functions as an adhesin and confers protective immunity in a lethal mouse model of Ots...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006784

    authors: Koralur MC,Ramaiah A,Dasch GA

    更新日期:2018-09-20 00:00:00

  • Imported endemic mycoses in Spain: Evolution of hospitalized cases, clinical characteristics and correlation with migratory movements, 1997-2014.

    abstract::Endemic mycoses are systemic fungal infections. Histoplasmosis is endemic in all temperate areas of the world; coccidioidomycosis and paracoccidioidomycosis are only present in the American continent. These pathogens are not present in Spain, but in the last years there has been an increase of reported cases due to mi...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006245

    authors: Molina-Morant D,Sánchez-Montalvá A,Salvador F,Sao-Avilés A,Molina I

    更新日期:2018-02-15 00:00:00

  • Leptospira Serovars for Diagnosis of Leptospirosis in Humans and Animals in Africa: Common Leptospira Isolates and Reservoir Hosts.

    abstract::The burden of leptospirosis in humans and animals in Africa is higher than that reported from other parts of the world. However, the disease is not routinely diagnosed in the continent. One of major factors limiting diagnosis is the poor availability of live isolates of locally circulating Leptospira serovars for incl...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0004251

    authors: Mgode GF,Machang'u RS,Mhamphi GG,Katakweba A,Mulungu LS,Durnez L,Leirs H,Hartskeerl RA,Belmain SR

    更新日期:2015-12-01 00:00:00

  • Loss of Glycosaminoglycan Receptor Binding after Mosquito Cell Passage Reduces Chikungunya Virus Infectivity.

    abstract::Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that can cause fever and chronic arthritis in humans. CHIKV that is generated in mosquito or mammalian cells differs in glycosylation patterns of viral proteins, which may affect its replication and virulence. Herein, we compare replication, pathogenicity,...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0004139

    authors: Acharya D,Paul AM,Anderson JF,Huang F,Bai F

    更新日期:2015-10-20 00:00:00

  • Multi-disease data management system platform for vector-borne diseases.

    abstract:BACKGROUND:Emerging information technologies present new opportunities to reduce the burden of malaria, dengue and other infectious diseases. For example, use of a data management system software package can help disease control programs to better manage and analyze their data, and thus enhances their ability to carry ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0001016

    authors: Eisen L,Coleman M,Lozano-Fuentes S,McEachen N,Orlans M,Coleman M

    更新日期:2011-03-29 00:00:00

  • Stratified sero-prevalence revealed overall high disease burden of dengue but suboptimal immunity in younger age groups in Pune, India.

    abstract:BACKGROUND:In India, dengue disease is emerging as the most important vector borne public health problem due to rapid and unplanned urbanization, high human density and week management of the disease. Clinical cases are grossly underreported and not much information is available on prevalence and incidence of the disea...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006657

    authors: Mishra AC,Arankalle VA,Gadhave SA,Mahadik PH,Shrivastava S,Bhutkar M,Vaidya VM

    更新日期:2018-08-06 00:00:00

  • Household-level expenditure on protective measures against mosquitoes on the island of La Réunion, France.

    abstract:BACKGROUND:For decades La Réunion has experienced a number of epidemics that have resulted in efforts to control the density of Aedes species on this Island. This study was conducted to assess household-level expenditure on protective measures against mosquito nuisance on the Island of La Réunion in 2012. METHODOLOGY/...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0002609

    authors: Thuilliez J,Bellia C,Dehecq JS,Reilhes O

    更新日期:2014-01-02 00:00:00

  • Autochthonous Chikungunya Transmission and Extreme Climate Events in Southern France.

    abstract:BACKGROUND:Extreme precipitation events are increasing as a result of ongoing global warming, but controversy surrounds the relationship between flooding and mosquito-borne diseases. A common view among the scientific community and public health officers is that heavy rainfalls have a flushing effect on breeding sites,...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0003854

    authors: Roiz D,Boussès P,Simard F,Paupy C,Fontenille D

    更新日期:2015-06-16 00:00:00

  • Value of Routine Dengue Diagnostic Tests in Urine and Saliva Specimens.

    abstract:BACKGROUND:Dengue laboratory diagnosis is essentially based on detection of the virus, its components or antibodies directed against the virus in blood samples. Blood, however, may be difficult to draw in some patients, especially in children, and sampling during outbreak investigations or epidemiological studies may f...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0004100

    authors: Andries AC,Duong V,Ly S,Cappelle J,Kim KS,Lorn Try P,Ros S,Ong S,Huy R,Horwood P,Flamand M,Sakuntabhai A,Tarantola A,Buchy P

    更新日期:2015-09-25 00:00:00

  • Prevalence and intensity of soil-transmitted helminthiasis, prevalence of malaria and nutritional status of school going children in honduras.

    abstract:BACKGROUND:Many small studies have been done in Honduras estimating soil-transmitted helminthiasis (STH) prevalence but a country-wide study was last done in 2005. The country has the highest burden of malaria among all Central American countries. The present study was done to estimate country-wide STH prevalence and i...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0003248

    authors: Mejia Torres RE,Franco Garcia DN,Fontecha Sandoval GA,Hernandez Santana A,Singh P,Mancero Bucheli ST,Saboya M,Paz MY

    更新日期:2014-10-16 00:00:00

  • Functional genomics in sand fly-derived Leishmania promastigotes.

    abstract:BACKGROUND:Leishmania development in the sand fly gut leads to highly infective forms called metacyclic promastigotes. This process can be routinely mimicked in culture. Gene expression-profiling studies by transcriptome analysis have been performed with the aim of studying promastigote forms in the sand fly gut, as we...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审

    doi:10.1371/journal.pntd.0007288

    authors: Alcolea PJ,Alonso A,Molina R,Jiménez M,Myler PJ,Larraga V

    更新日期:2019-05-09 00:00:00

  • A chronic bioluminescent model of experimental visceral leishmaniasis for accelerating drug discovery.

    abstract:BACKGROUND:Visceral leishmaniasis is a neglected parasitic disease with no vaccine available and its pharmacological treatment is reduced to a limited number of unsafe drugs. The scarce readiness of new antileishmanial drugs is even more alarming when relapses appear or the occurrence of hard-to-treat resistant strains...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0007133

    authors: Álvarez-Velilla R,Gutiérrez-Corbo MDC,Punzón C,Pérez-Pertejo MY,Balaña-Fouce R,Fresno M,Reguera RM

    更新日期:2019-02-14 00:00:00

  • Japanese encephalitis-a pathological and clinical perspective.

    abstract::Japanese encephalitis (JE) is the leading form of viral encephalitis in Asia. It is caused by the JE virus (JEV), which belongs to the family Flaviviridae. JEV is endemic to many parts of Asia, where periodic outbreaks take hundreds of lives. Despite the catastrophes it causes, JE has remained a tropical disease uncom...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0000437

    authors: Ghosh D,Basu A

    更新日期:2009-09-29 00:00:00

  • On-going transmission of human onchocerciasis in the Massangam health district in the West Region of Cameroon: Better understanding transmission dynamics to inform changes in programmatic interventions.

    abstract:BACKGROUND:Massangam health district (HD), in the West Region of Cameroon, has received ivermectin mass drug administration (MDA) for 20 years, however there is evidence of continued high transmission of Onchocerca volvulus. In order to better understand the transmission dynamics in the HD and inform intervention strat...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006904

    authors: Bakajika D,Senyonjo L,Enyong P,Oye J,Biholong B,Elhassan E,Boakye D,Dixon R,Schmidt E

    更新日期:2018-11-14 00:00:00

  • The natural history of acute Ebola Virus Disease among patients managed in five Ebola treatment units in West Africa: A retrospective cohort study.

    abstract:INTRODUCTION:Previous studies of Ebola Virus Disease (EVD) have focused on clinical symptoms and Ebola virus (EBOV) cycle threshold (CT) values recorded at patient triage. Our study explores EVD symptoms and EBOV CT values from onset of illness to recovery or death in a diverse population of patients. METHODOLOGY/PRIN...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0005700

    authors: Skrable K,Roshania R,Mallow M,Wolfman V,Siakor M,Levine AC

    更新日期:2017-07-19 00:00:00

  • Dynamics of serological responses to defined recombinant proteins during Schistosoma mansoni infection in mice before and after the treatment with praziquantel.

    abstract::To eliminate schistosomiasis, appropriate diagnostic tests are required to monitor its prevalence and transmission, especially in the settings with low endemicity resulting from the consecutive mass drug administration. Antibodies that react with either crude soluble schistosome egg antigens or soluble worm antigen pr...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0008518

    authors: Mohammed ES,Nakamura R,Kalenda YD,Deloer S,Moriyasu T,Tanaka M,Fujii Y,Kaneko S,Hirayama K,Ibrahim AI,El-Seify MA,Metwally AM,Hamano S

    更新日期:2020-09-11 00:00:00

  • RNA interference in Fasciola gigantica: Establishing and optimization of experimental RNAi in the newly excysted juveniles of the fluke.

    abstract::Fasciolosis caused by Fasciola gigantica is a neglected tropical disease but a constraint on the growth and productivity of cattle, buffaloes and sheep in the tropical countries of Asia and Africa. Resistance to commonly used anthelmintics in Fasciola has increased the need to search for alternative therapeutic target...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006109

    authors: Anandanarayanan A,Raina OK,Lalrinkima H,Rialch A,Sankar M,Varghese A

    更新日期:2017-12-12 00:00:00