Abstract：
OBJECTIVE:Comparative study of type 2 diabetes and healthy controls by metabolomics methods to explore the pathogenesis of Type II diabetes. METHODS:Gas chromatography - mass spectrometry (GC-MS) with a variety of multivariate statistical analysis methods to the healthy control group 58 cases, 68 cases of Type II diabetes group were analyzed. Chromatographic conditions: DB-5MS column; the carrier gas He; flow rate of 1 mL·min(-1), the injection volume 1 uL; split ratio is 100: 1. MS conditions: electron impact (EI) ion source, an auxiliary temperature of 280°C, the ion source 230°C, quadrupole 150°C; mass scan range 30~600 mAu. RESULTS:Established analytical method based on urine metabolomics GC-MS of Type II diabetes, determine the urine succinic acid, L-leucine, L-isoleucine, tyrosine, slanine, acetoace acid, mannose, L-isoleucine, L-threonine, Phenylalanine, fructose, D-glucose, palmi acid, oleic acid and arachidonic acid were significantly were significantly changed. CONCLUSION:Based on metabolomics of GC-MS detection and analysis metabolites can be found differences between type 2 diabetes and healthy control group, PCA diagram can effectively distinguish Type II diabetes and healthy control group, with load diagrams and PLS-DA VIP value metabolite screening, the resulting differences in metabolic pathways involved metabolites, including amino acid metabolism, lipid metabolism, glucose metabolism and energy metabolism.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Zhang N,Geng F,Hu ZH,Liu B,Wang YQ,Liu JC,Qi YH,Li LJsubject
Has Abstractpub_date
2016-07-15 00:00:00pages
2889-96issue
7issn
1943-8141journal_volume
8pub_type
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2015-08-15 00:00:00
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
pub_type： 杂志文章
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更新日期：2020-12-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
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journal_title：American journal of translational research
pub_type： 杂志文章
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更新日期：2019-05-15 00:00:00
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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更新日期：2018-11-15 00:00:00
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
pub_type： 杂志文章,评审
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journal_title：American journal of translational research
pub_type： 杂志文章,评审
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journal_title：American journal of translational research
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doi：
更新日期：2012-01-01 00:00:00
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-07-15 00:00:00
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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doi：
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