FSCB phosphorylation regulates mouse spermatozoa capacitation through suppressing SUMOylation of ROPN1/ROPN1L.

Abstract:

:Fibrous sheath CABYR binding protein (FSCB) is regulated by protein kinase A (PKA)-mediated tyrosine phosphorylation in the spermatozoa capacitation. Recently, we showed that FSCB phosphorylation activated spermatozoa motility. Nevertheless, the underlying mechanisms have not been completely elucidated. Here, we showed that FSCB phosphorylation inhibited SUMOylation of two crucial proteins ROPN1/ROPN1L that are associated with PKA/A kinase activity and spermatozoa motility. Suppression of SUMOylation of ROPN1/ROPN1L mimicked the effects of FSCB phosphorylation on spermatozoa motility. Immunoprecipitation assay showed that phosphorylated FSCB had a significantly higher affinity to ROPN1/ROPN1L than non-phosphorylated FSCB. Together, our data suggest that FSCB phosphorylation may regulate mouse spermatozoa capacitation through suppressing SUMOylation of ROPN1/ROPN1L, which sheds new light on creating a therapeutic strategy targeting FSCB phosphorylation in the study of infertility.

journal_name

Am J Transl Res

authors

Zhang X,Chen M,Yu R,Liu B,Tian Z,Liu S

subject

Has Abstract

pub_date

2016-06-15 00:00:00

pages

2776-82

issue

6

issn

1943-8141

journal_volume

8

pub_type

杂志文章
  • Low concentration of chloroquine enhanced efficacy of cisplatin in the treatment of human ovarian cancer dependent on autophagy.

    abstract:BACKGROUND:Cisplatin is a common used anti-tumor drug in ovarian cancer therapy with potent effect. Studies have reported that autophagy works as a cell-survival process in cancer, chloroquine has been added to various chemotherapeutic drugs. In the current study, we aim to evaluate whether chloroquine can enhance the ...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Zhu J,Zheng Y,Zhang H,Zhu J,Sun H

    更新日期:2017-09-15 00:00:00

  • Single-cell rna seq analysis identifies the biomarkers and differentiation of chondrocyte in human osteoarthritis.

    abstract:BACKGROUND:Single-cell RNA sequencing (scRNA-seq) was recently adopted for exploring molecular programmes and lineage progression patterns of pathogenesis of important diseases. In this study, scRNA-seq was used to identify potential markers for chondrocytes in osteoarthritis (OA) and to explore the function of differe...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Zhang X,Huang N,Huang R,Wang L,Ke Q,Cai L,Wu S

    更新日期:2020-11-15 00:00:00

  • Glioma-associated oncogene homolog 1 stimulates FOXP3 to promote non-small cell lung cancer stemness.

    abstract::Glioma-associated oncogene homolog 1 (GLI1), an oncogenic molecule in non-small cell lung cancer (NSCLC), promotes the growth of NSCLC by enhancing lung cancer stem cells (LCSCs). However, the mechanism responsible remains unknown. FOXP3 is known to maintain LCSCs. The aim of this study was to explore whether GLI1 enh...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Qi H,Li W,Zhang J,Chen J,Peng J,Liu Y,Yang S,Du J,Long X,Ng CS,Li MY,Chen GG

    更新日期:2020-05-15 00:00:00

  • Tert-butylhydroquinone ameliorates doxorubicin-induced cardiotoxicity by activating Nrf2 and inducing the expression of its target genes.

    abstract:UNLABELLED:Oxidative stress plays an important role in doxorubicin (DOX)-induced cardiotoxicity. Nuclear factor E2-related factor-2 (Nrf2) is a transcription factor that orchestrates the antioxidant and cytoprotective responses to oxidative stress. In the present study, we tested whether tert-butylhydroquinone (tBHQ) c...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Wang LF,Su SW,Wang L,Zhang GQ,Zhang R,Niu YJ,Guo YS,Li CY,Jiang WB,Liu Y,Guo HC

    更新日期:2015-10-15 00:00:00

  • LncRNA TUG1 promotes the intervertebral disc degeneration and nucleus pulposus cell apoptosis though modulating miR-26a/HMGB1 axis and regulating NF-κB activation.

    abstract:AIMS:This study was to investigate the effect of TUG1 on apoptosis and ECM degradation of human degenerative intervertebral disc nucleus pulposus cells (NPCs) and its mechanism. METHODS:Human degenerative intervertebral disc NP tissues were obtained from 10 patients with lumbar disc herniation (LDH) who underwent lumb...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Tang N,Dong Y,Xiao T,Zhao H

    更新日期:2020-09-15 00:00:00

  • Oridonin ameliorates lipopolysaccharide/D-galactosamine-induced acute liver injury in mice via inhibition of apoptosis.

    abstract::We investigated the protective effects exerted by oridonin, the main active constituent of the Chinese medicinal herb Rabdosiarubescens, against lipopolysaccharide (LPS)/D-galactosamine (D-Gal)-induced acute liver injury (ALI). An ALI model was induced in mice using LPS (40 μg/0.5 ml) and D-Gal (5 mg/0.5 ml). The mice...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Deng Y,Chen C,Yu H,Diao H,Shi C,Wang Y,Li G,Shi M

    更新日期:2017-09-15 00:00:00

  • Erratum: Exogenous glutathione contributes to cisplatin resistance in lung cancer A549 cells.

    abstract::[This corrects the article on p. 1295 in vol. 10, PMID: 29887946.]. ...

    journal_title:American journal of translational research

    pub_type: 杂志文章,已发布勘误

    doi:

    authors: Lan D,Wang L,He R,Ma J,Bin Y,Chi X,Chen G,Cai Z

    更新日期:2018-07-15 00:00:00

  • Vitamin D and alopecia areata: possible roles in pathogenesis and potential implications for therapy.

    abstract::Alopecia areata is characterized by the loss of hair on the scalp and elsewhere on the body. It affects approximately 2% of the general population. It is believed to be an autoimmune disease. However, its pathogenesis remains incompletely understood. Recent studies have revealed a substantial link between vitamin D an...

    journal_title:American journal of translational research

    pub_type: 杂志文章,评审

    doi:

    authors: Lin X,Meng X,Song Z

    更新日期:2019-09-15 00:00:00

  • Upregulation of TSPAN12 is associated with the colorectal cancer growth and metastasis.

    abstract::Tetraspanin 12 (TSPAN12), as an earliest member of tetraspanin family, has been recently shown to be highly expressed in several malignant tumors, such as lung cancer and breast cancer, which plays an important role in regulating cell proliferation, migration and invasion. However, the functional roles of TSPAN12 in c...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Liu J,Chen C,Li G,Chen D,Zhou Q

    更新日期:2017-02-15 00:00:00

  • Geranylgeranylation promotes proliferation, migration and invasion of gastric cancer cells through the YAP signaling pathway.

    abstract::Geranylgeranylation (GGylation) is a lipid modification process of signaling proteins. Currently, very little is known about the GGylation signaling for gastric cancer cell proliferation and migration. In this report, we found that inhibition of GGylation by the mevalonate pathway inhibitor atorvastatin and the gerany...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Wei J,Peng K,Zhu J,Wang L,Han T,Sun A,Shao G,Liu H,Yang W,Lin Q

    更新日期:2020-09-15 00:00:00

  • Whether CD44 is an applicable marker for glioma stem cells.

    abstract::Glioblastoma multiforme (GBM) is one of the most malignant and aggressive brain tumors with great amount of hyaluronan (HA) secretion and CD44 overexpression (HA receptor). CD44 has been suggested as a cancer stem cells (CSCs) marker. However, several clinical studies have indicated that CD44low glioma cell exhibit CS...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Wang HH,Liao CC,Chow NH,Huang LL,Chuang JI,Wei KC,Shin JW

    更新日期:2017-11-15 00:00:00

  • De novo cartilage growth after implantation of a 3-D-printed tracheal graft in a porcine model.

    abstract:BACKGROUND:Experiments were conducted on the assumption that vivid chondrogenesis would be boosted in vivo following previously preliminary chondrogenesis in a mesenchymal stem cell (MSC)-rich entire umbilical cord (UC) in vitro. METHODS:Virtual 3-D tracheal grafts were generated by using a profile obtained by scannin...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Shai SE,Lai YL,Hung YW,Hsieh CW,Huang BJ,Su KC,Wang CH,Hung SC

    更新日期:2020-07-15 00:00:00

  • Inhibition of KiSS-1 on metastasis of nasopharyngeal carcinoma implant tumor in nude mice.

    abstract:BACKGROUND:Nasopharyngeal carcinoma (NPC) is a kind of head-neck malignant neoplasm originated from the nasopharyngeal epithelium and is mainly prevalent in Southern China and Southeast Asia countries. KiSS-1 is an inhibitor of tumor metastasis in a range of cancers. METHODS:We establish a cell substrain of SUNE-1-5-8...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Wang GM,Liu JF,Zhang L,Sun Q,Zhou Y,Xu HB,Zhang YJ,Cai F,Cheng ZN,Xiang P,Jiang H

    更新日期:2019-02-15 00:00:00

  • Physiological regulation of Akt activity and stability.

    abstract::The serine/threonine protein kinase B (PKB, also known as Akt) constitutes an important node in diverse signaling cascades downstream of growth factor receptor tyrosine kinases. Akt plays an essential role in cell survival, growth, migration, proliferation, polarity, and metabolism (lipid and glucose); cell cycle prog...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Liao Y,Hung MC

    更新日期:2010-01-01 00:00:00

  • Proteasome inhibition enhances the killing effect of BikDD gene therapy.

    abstract::BikDD, a phosphorylation-mimic mutant of pro-apoptotic protein Bik, elicits strong apoptosis in cancer cells when introduced via an expression platform termed VP16-GAL4-WPRE integrated systemic amplifier (VISA) under the control of a cancer-specific promoter both in vitro and in vivo. C-VISA-BikDD expression plasmid e...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Sun Y,Ponz-Sarvise M,Chang SS,Chang WC,Chen CH,Hsu JL,Hung MC

    更新日期:2015-02-15 00:00:00

  • Diffusion-weighted imaging with background body signal suppression (DWIBS) distinguishes benign lesions from malignant pulmonary solitary lesions.

    abstract::This study aimed to determine applicable value of DWIBS in diagnosis of solitary pulmonary lesions. This study involved 32 solitary lung disease patients. T1W1, T2W1, T2WI-SPAIR were examined using MRI scanner and analyzed with View-forum 6.0 workstation. Imaging characteristics of pulmonary solitary lesions on DWIBS ...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Zhao C,Deng D,Ye W,Long L,Lu Y,Wei Y

    更新日期:2021-01-15 00:00:00

  • Lost in translation: animal models and clinical trials in cancer treatment.

    abstract::Due to practical and ethical concerns associated with human experimentation, animal models have been essential in cancer research. However, the average rate of successful translation from animal models to clinical cancer trials is less than 8%. Animal models are limited in their ability to mimic the extremely complex ...

    journal_title:American journal of translational research

    pub_type: 杂志文章,评审

    doi:

    authors: Mak IW,Evaniew N,Ghert M

    更新日期:2014-01-15 00:00:00

  • Discoidin domain receptor 1 activity drives an aggressive phenotype in bladder cancer.

    abstract::Discoidin domain receptor 1 (DDR1) is a receptor tyrosine kinase which utilizes collagen as a ligand to regulate the interaction between cancer cells and tumor stroma. However, the clinical relevance of DDR1 expression in bladder cancer as well as its molecular regulation have not been previously investigated. Here, w...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Xie X,Rui W,He W,Shao Y,Sun F,Zhou W,Wu Y,Zhu Y

    更新日期:2017-05-15 00:00:00

  • ROC-supervised principal component analysis in connection with the diagnosis of diseases.

    abstract::Principal component analysis (PCA) is a data analysis method that can deal with large volumes of data. Owing to the complexity and volume of the data generated by today's advanced technologies in genomics, pro-teomics, and metabolomics, PCA has become predominant in the medical sciences. Despite its popularity, PCA le...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Nikas JB,Low WC

    更新日期:2011-02-01 00:00:00

  • Salvianolic acid A attenuates cerebral ischemia/reperfusion injury induced rat brain damage, inflammation and apoptosis by regulating miR-499a/DDK1.

    abstract:BACKGROUND AND OBJECTIVES:Salvianolic acid A (SAA) is a main component derived from Salvia miltiorrhiza and has been revealed to protect against cerebral ischemia/reperfusion injury (CIRI). The present study was designed to evaluate the neuroprotective role of SAA in CIRI and explore its underlying mechanism in vivo an...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Zhao J,Li L,Fang G

    更新日期:2020-07-15 00:00:00

  • HOE-642 improves the protection of hypothermia on neuronal mitochondria after cardiac arrest in rats.

    abstract::HOE-642 has been shown to provide significant protection in a variety of models of cerebral and myocardial ischemia/reperfusion injury. In this study, we examined the impact of HOE-642, a selective Na+/H+ exchanger 1 inhibitor, with or without hypothermia on neuronal and neuronal mitochondrial function during resuscit...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Wei L,Zhang P,Hu Y,Zhao W,Liu X,Wang X,Han F

    更新日期:2020-05-15 00:00:00

  • Ellagic acid inhibits cell proliferation, migration, and invasion in melanoma via EGFR pathway.

    abstract::Ellagic acid (EA), a polyphenolic compound from pomegranate fruit extracts, has been reported to possess anti-proliferation, pro-apoptosis, and anti-invasion effects on many cancers. However, its effect on melanoma is yet to be clarified. In the present study, we investigated the anti-cancer effects of EA on melanoma ...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Wang F,Chen J,Xiang D,Lian X,Wu C,Quan J

    更新日期:2020-05-15 00:00:00

  • Neuroprotective effect of miR-665 against sevoflurane anesthesia-induced cognitive dysfunction in rats through PI3K/Akt signaling pathway by targeting insulin-like growth factor 2.

    abstract::The aim of this study was to investigate the in vivo and in vitro effects of miR-665 on sevoflurane anesthesia-induced cognitive dysfunction. SH-SY5Y cells and male SD rats were treated with sevoflurane to simulate anesthesia-induced cognitive dysfunction. The cells and rats both were transfected with a miR-665 mimic,...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Lu X,Lv S,Mi Y,Wang L,Wang G

    更新日期:2017-03-15 00:00:00

  • Glutaminase inhibitor compound 968 inhibits cell proliferation and sensitizes paclitaxel in ovarian cancer.

    abstract:OBJECTIVE:Our overall goal was to investigate the anti-tumor activity of the glutaminase 1 (GLS1) Inhibitor compound 968 in ovarian cancer cells. The human ovarian cancer cell lines, HEY, SKOV3 and IGROV-1 were used. Cell proliferation was assessed by MTT assay after treatment with compound 968. Cell cycle progression ...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Yuan L,Sheng X,Clark LH,Zhang L,Guo H,Jones HM,Willson AK,Gehrig PA,Zhou C,Bae-Jump VL

    更新日期:2016-10-15 00:00:00

  • Silencing long noncoding RNA PVT1 inhibits tumorigenesis and cisplatin resistance of colorectal cancer.

    abstract::Long noncoding RNA plasmacytoma variant translocation 1 (PVT1) plays pivotal roles in tumorigenesis of many cancers, including colorectal cancer (CRC). However, the clinical significance and the biological functions of PVT1 in CRC remain largely unknown. In this study, we found that PVT1 was highly expressed in CRC ti...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Ping G,Xiong W,Zhang L,Li Y,Zhang Y,Zhao Y

    更新日期:2018-01-15 00:00:00

  • Analysis of the effects of preservative-free tafluprost on the tear proteome.

    abstract::The purpose of the present study was to assess the ocular surface health status in primary open angle glaucoma (POAG) patients switching from topical application of preserved latanoprost (LT) to preservative-free tafluprost (PFT) by tear proteomic monitoring. Tear fluid of POAG patients showing dry eye symptoms, using...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Funke S,Beck S,Lorenz K,Kotterer M,Wolters D,Perumal N,Pfeiffer N,Grus FH

    更新日期:2016-10-15 00:00:00

  • MicroRNA-129-5p inhibits human glioma cell proliferation and induces cell cycle arrest by directly targeting DNMT3A.

    abstract:BACKGROUND:Glioma is the most common malignant tumor in the adult human brain and has one of the lowest patient survival rates. MicroRNAs (miRNAs) play important roles in the development of cancers, including glioma, and potentially have valuable therapeutic applications in glioma; however, their specific functions and...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Gu X,Gong H,Shen L,Gu Q

    更新日期:2018-09-15 00:00:00

  • Long non-coding RNA AK001058 regulates tumor growth and angiogenesis in colorectal cancer via methylation of ADAMTS12.

    abstract::Colorectal cancer, a common gastrointestinal malignant tumor, has been a leading cause of cancer related deaths. Long non-coding RNAs (lncRNAs) play an important role in regulating cancer development. The aim of this study was to investigate the role and potential mechanism of lncRNA AK001058 in colorectal cancer. To ...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Zheng S,Lin F,Zhang M,Mu N,Ge X,Fu J

    更新日期:2019-09-15 00:00:00

  • Quercetin stimulates osteogenic differentiation of bone marrow stromal cells through miRNA-206/connexin 43 pathway.

    abstract::The quantity and function of osteoblasts require continuous osteogenic differentiation of bone marrow mesenchymal stem cells. Recent evidence suggests that microRNAs (miRNAs) act as important post-transcriptional regulators in a wide range of biological processes, including osteoblastic differentiation. Quercetin has ...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Zhang Q,Chang B,Zheng G,Du S,Li X

    更新日期:2020-05-15 00:00:00

  • Gender, age and comorbidities as the main prognostic factors in patients with COVID-19 pneumonia.

    abstract::The coronavirus disease 2019 (COVID-19) pandemic has spread to almost all countries. The currently reported epidemiological statistics show that age, gender, and type of comorbidities may be high-risk factors for critically ill patients with COVID-19. However, there is no comprehensive analysis of these risk factors. ...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Mi J,Zhong W,Huang C,Zhang W,Tan L,Ding L

    更新日期:2020-10-15 00:00:00