Abstract:
INTRODUCTION:Core cerebrospinal fluid (CSF) biomarkers - Aβ42, Tau, and phosphorylated Tau (pTau) - have been recently incorporated in the revised criteria for Alzheimer's disease (AD). However, their widespread clinical application lacks standardization. Pre-analytical sample handling and storage play an important role in the reliable measurement of these biomarkers across laboratories. AIM:In this study, we aim to surpass the efforts from previous studies, by employing a multicenter approach to assess the impact of less studied CSF pre-analytical confounders in AD-biomarkers quantification. METHODS:Four different centers participated in this study and followed the same established protocol. CSF samples were analyzed for three biomarkers (Aβ42, Tau, and pTau) and tested for different spinning conditions [temperature: room temperature (RT) vs. 4°C; speed: 500 vs. 2000 vs. 3000 g], storage volume variations (25, 50, and 75% of tube total volume), as well as freezing-thaw cycles (up to five cycles). The influence of sample routine parameters, inter-center variability, and relative value of each biomarker (reported as normal/abnormal) was analyzed. RESULTS:Centrifugation conditions did not influence biomarkers levels, except for samples with a high CSF total protein content, where either non-centrifugation or centrifugation at RT, compared to 4°C, led to higher Aβ42 levels. Reducing CSF storage volume from 75 to 50% of total tube capacity decreased Aβ42 concentration (within analytical CV of the assay), whereas no change in Tau or pTau was observed. Moreover, the concentration of Tau and pTau appears to be stable up to five freeze-thaw cycles, whereas Aβ42 levels decrease if CSF is freeze-thawed more than three times. CONCLUSION:This systematic study reinforces the need for CSF centrifugation at 4°C prior to storage and highlights the influence of storage conditions in Aβ42 levels. This study contributes to the establishment of harmonized standard operating procedures that will help reducing inter-lab variability of CSF-AD biomarkers evaluation.
journal_name
Front Neuroljournal_title
Frontiers in neurologyauthors
Leitão MJ,Baldeiras I,Herukka SK,Pikkarainen M,Leinonen V,Simonsen AH,Perret-Liaudet A,Fourier A,Quadrio I,Veiga PM,de Oliveira CRdoi
10.3389/fneur.2015.00153subject
Has Abstractpub_date
2015-07-08 00:00:00pages
153issn
1664-2295journal_volume
6pub_type
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journal_title:Frontiers in neurology
pub_type: 杂志文章
doi:10.3389/fneur.2018.00141
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abstract::There is a need for biomarkers for amyotrophic lateral sclerosis (ALS), to support the diagnosis of the disease, to predict disease progression and to track disease activity and treatment responses. Over the last decade multiple studies have investigated the potential of neurofilament levels, both in cerebrospinal flu...
journal_title:Frontiers in neurology
pub_type: 杂志文章,评审
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abstract::Background: Educational interventions are needed to overcome knowledge-to-action gaps in clinical care. We previously tested the feasibility and potential efficacy of an educational intervention that facilitates treatment decisions in multiple sclerosis care. A demonstration of the usability of such an intervention is...
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pub_type: 杂志文章
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abstract::Ictal fear-a relatively rare phenomenon-is a semiological characteristic of epilepsy. Most patients with epilepsy with ictal fear have an epileptic zone in the mesial temporal lobe, which is the classical brain area involved in emotion processing. Herein, we report a case of epilepsy with ictal fear as the first manif...
journal_title:Frontiers in neurology
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abstract::Background: Given the lethal severity of amyotrophic lateral sclerosis (ALS), the aim of this study was to illuminate the coherence of depression and death anxiety in both ALS patients and caregivers and in how far patients and caregivers are influenced by the mindset of their respective counterpart. Methods: 30 coupl...
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pub_type: 杂志文章
doi:10.3389/fneur.2018.01035
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abstract::Background: Caregiver strain is recognized globally with Parkinson's disease (PD). Comparatively little is understood about caregiver burden and strain in Asia. Objective: To investigate caregiver strain for families living with PD in Singapore, in light of international data. Methods: Ninety-four caregivers were recr...
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journal_title:Frontiers in neurology
pub_type: 杂志文章
doi:10.3389/fneur.2018.01151
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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pub_type: 杂志文章
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doi:10.3389/fneur.2018.00573
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abstract::Background: Over the last few years there has been growing interest in use of visual measures as useful tools for multiple sclerosis (MS) prognosis and tracking. Optic neuritis (ON) being a prevalent and often-presenting symptom of the disease, as well as the high occurrence rate of posterior visual system damage inde...
journal_title:Frontiers in neurology
pub_type: 杂志文章
doi:10.3389/fneur.2019.00455
更新日期:2019-05-03 00:00:00
abstract::Shame and embarrassment related to Parkinson's disease (PD) are rarely addressed in clinical practice nor studied in neuroscience research, partly because no specific tool exists to detect them in PD. Objective: To develop a self-applied assessment tool of shame and embarrassment specifically related to PD or its trea...
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pub_type: 杂志文章
doi:10.3389/fneur.2020.00779
更新日期:2020-07-31 00:00:00
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pub_type: 杂志文章
doi:10.3389/fneur.2018.00321
更新日期:2018-05-07 00:00:00
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journal_title:Frontiers in neurology
pub_type:
doi:10.3389/fneur.2019.00620
更新日期:2019-06-12 00:00:00
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pub_type: 杂志文章
doi:10.3389/fneur.2019.00563
更新日期:2019-06-05 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2020-11-17 00:00:00
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doi:10.3389/fneur.2017.00032
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abstract::[This corrects the article DOI: 10.3389/fneur.2019.00129.]. ...
journal_title:Frontiers in neurology
pub_type: 杂志文章,已发布勘误
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更新日期:2019-03-27 00:00:00
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更新日期:2018-03-27 00:00:00
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pub_type:
doi:10.3389/fneur.2017.00624
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