Identification of microRNA genes in three opisthorchiids.


BACKGROUND:Opisthorchis felineus, O. viverrini, and Clonorchis sinensis (family Opisthorchiidae) are parasitic flatworms that pose a serious threat to humans in some countries and cause opisthorchiasis/clonorchiasis. Chronic disease may lead to a risk of carcinogenesis in the biliary ducts. MicroRNAs (miRNAs) are small noncoding RNAs that control gene expression at post-transcriptional level and are implicated in the regulation of various cellular processes during the parasite- host interplay. However, to date, the miRNAs of opisthorchiid flukes, in particular those essential for maintaining their complex biology and parasitic mode of existence, have not been satisfactorily described. METHODOLOGY/PRINCIPAL FINDINGS:Using a SOLiD deep sequencing-bioinformatic approach, we identified 43 novel and 18 conserved miRNAs for O. felineus (miracidia, metacercariae and adult worms), 20 novel and 16 conserved miRNAs for O. viverrini (adult worms), and 33 novel and 18 conserved miRNAs for C. sinensis (adult worms). The analysis of the data revealed differences in the expression level of conserved miRNAs among the three species and among three the developmental stages of O. felineus. Analysis of miRNA genes revealed two gene clusters, one cluster-like region and one intronic miRNA in the genome. The presence and structure of the two gene clusters were validated using a PCR-based approach in the three flukes. CONCLUSIONS:This study represents a comprehensive description of miRNAs in three members of the family Opistorchiidae, significantly expands our knowledge of miRNAs in multicellular parasites and provides a basis for understanding the structural and functional evolution of miRNAs in these metazoan parasites. Results of this study also provides novel resources for deeper understanding the complex parasite biology, for further research on the pathogenesis and molecular events of disease induced by the liver flukes. The present data may also facilitate the development of novel approaches for the prevention and treatment of opisthorchiasis/clonorchiasis.


PLoS Negl Trop Dis


Ovchinnikov VY,Afonnikov DA,Vasiliev GV,Kashina EV,Sripa B,Mordvinov VA,Katokhin AV




Has Abstract


2015-04-21 00:00:00














  • LABCG2, a new ABC transporter implicated in phosphatidylserine exposure, is involved in the infectivity and pathogenicity of Leishmania.

    abstract::Leishmaniasis is a neglected disease produced by the intracellular protozoan parasite Leishmania. In the present study, we show that LABCG2, a new ATP-binding cassette half-transporter (ABCG subfamily) from Leishmania, is involved in parasite virulence. Down-regulation of LABCG2 function upon expression of an inactive...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Campos-Salinas J,León-Guerrero D,González-Rey E,Delgado M,Castanys S,Pérez-Victoria JM,Gamarro F

    更新日期:2013-04-25 00:00:00

  • External quality assessment study for ebolavirus PCR-diagnostic promotes international preparedness during the 2014 - 2016 Ebola outbreak in West Africa.

    abstract::During the recent Ebola outbreak in West Africa several international mobile laboratories were deployed to the mainly affected countries Guinea, Sierra Leone and Liberia to provide ebolavirus diagnostic capacity. Additionally, imported cases and small outbreaks in other countries required global preparedness for Ebola...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,多中心研究


    authors: Ellerbrok H,Jacobsen S,Patel P,Rieger T,Eickmann M,Becker S,Günther S,Naidoo D,Schrick L,Keeren K,Targosz A,Teichmann A,Formenty P,Niedrig M

    更新日期:2017-05-01 00:00:00

  • Directed evaluation of enterotoxigenic Escherichia coli autotransporter proteins as putative vaccine candidates.

    abstract:BACKGROUND:Enterotoxigenic Escherichia coli (ETEC) is a major diarrheal pathogen in developing countries, where it accounts for millions of infections and hundreds of thousands of deaths annually. While vaccine development to prevent diarrheal illness due to ETEC is feasible, extensive effort is needed to identify cons...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Harris JA,Roy K,Woo-Rasberry V,Hamilton DJ,Kansal R,Qadri F,Fleckenstein JM

    更新日期:2011-12-01 00:00:00

  • Comprehensive Survey of Domiciliary Triatomine Species Capable of Transmitting Chagas Disease in Southern Ecuador.

    abstract:BACKGROUND:Chagas disease is endemic to the southern Andean region of Ecuador, an area with one of the highest poverty rates in the country. However, few studies have looked into the epidemiology, vectors and transmission risks in this region. In this study we describe the triatomine household infestation in Loja provi...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Grijalva MJ,Villacis AG,Ocaña-Mayorga S,Yumiseva CA,Moncayo AL,Baus EG

    更新日期:2015-10-06 00:00:00

  • A Systematic Review of the Mortality from Untreated Leptospirosis.

    abstract:BACKGROUND:Leptospirosis occurs worldwide, but the global incidence of human disease and its mortality are not well understood. Many patients are undiagnosed and untreated due to its non-specific symptoms and a lack of access to diagnostics. This study systematically reviews the literature to clarify the mortality from...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Taylor AJ,Paris DH,Newton PN

    更新日期:2015-06-25 00:00:00

  • Insights into the sand fly saliva: Blood-feeding and immune interactions between sand flies, hosts, and Leishmania.

    abstract:BACKGROUND:Leishmaniases are parasitic diseases present worldwide that are transmitted to the vertebrate host by the bite of an infected sand fly during a blood feeding. Phlebotomine sand flies inoculate into the mammalian host Leishmania parasites embedded in promastigote secretory gel (PSG) with saliva, which is comp...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Lestinova T,Rohousova I,Sima M,de Oliveira CI,Volf P

    更新日期:2017-07-13 00:00:00

  • The use of a mobile laboratory unit in support of patient management and epidemiological surveillance during the 2005 Marburg Outbreak in Angola.

    abstract:BACKGROUND:Marburg virus (MARV), a zoonotic pathogen causing severe hemorrhagic fever in man, has emerged in Angola resulting in the largest outbreak of Marburg hemorrhagic fever (MHF) with the highest case fatality rate to date. METHODOLOGY/PRINCIPAL FINDINGS:A mobile laboratory unit (MLU) was deployed as part of the...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Grolla A,Jones SM,Fernando L,Strong JE,Ströher U,Möller P,Paweska JT,Burt F,Pablo Palma P,Sprecher A,Formenty P,Roth C,Feldmann H

    更新日期:2011-05-01 00:00:00

  • Leptospira Interrogans induces fibrosis in the mouse kidney through Inos-dependent, TLR- and NLR-independent signaling pathways.

    abstract:BACKGROUND:Leptospira (L.) interrogans are bacteria responsible for a worldwide reemerging zoonosis. Rodents carry L. interrogans asymptomatically in their kidneys and excrete bacteria in the urine, contaminating the environment. Humans get infected through skin contact and develop a mild or severe leptospirosis that m...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Fanton d'Andon M,Quellard N,Fernandez B,Ratet G,Lacroix-Lamandé S,Vandewalle A,Boneca IG,Goujon JM,Werts C

    更新日期:2014-01-30 00:00:00

  • Insights into the trypanosome-host interactions revealed through transcriptomic analysis of parasitized tsetse fly salivary glands.

    abstract::The agents of sleeping sickness disease, Trypanosoma brucei complex parasites, are transmitted to mammalian hosts through the bite of an infected tsetse. Information on tsetse-trypanosome interactions in the salivary gland (SG) tissue, and on mammalian infective metacyclic (MC) parasites present in the SG, is sparse. ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Telleria EL,Benoit JB,Zhao X,Savage AF,Regmi S,Alves e Silva TL,O'Neill M,Aksoy S

    更新日期:2014-04-24 00:00:00

  • Serological evidence of exposure to Rift Valley, Dengue and Chikungunya Viruses among agropastoral communities in Manyara and Morogoro regions in Tanzania: A community survey.

    abstract::Tanzania has recently experienced outbreaks of dengue in two coastal regions of Dar es Salaam and Tanga. Chikungunya and Rift Valley Fever outbreaks have also been recorded in the past decade. Little is known on the burden of the arboviral disease causing viruses (Dengue, Rift Valley and Chikungunya) endemically in th...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Budodo RM,Horumpende PG,Mkumbaye SI,Mmbaga BT,Mwakapuja RS,Chilongola JO

    更新日期:2020-07-20 00:00:00

  • Interaction of ATP with a small heat shock protein from Mycobacterium leprae: effect on its structure and function.

    abstract::Adenosine-5'-triphosphate (ATP) is an important phosphate metabolite abundantly found in Mycobacterium leprae bacilli. This pathogen does not derive ATP from its host but has its own mechanism for the generation of ATP. Interestingly, this molecule as well as several antigenic proteins act as bio-markers for the detec...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Nandi SK,Chakraborty A,Panda AK,Ray SS,Kar RK,Bhunia A,Biswas A

    更新日期:2015-03-26 00:00:00

  • Identification of Novel Chemical Scaffolds Inhibiting Trypanothione Synthetase from Pathogenic Trypanosomatids.

    abstract:BACKGROUND:The search for novel chemical entities targeting essential and parasite-specific pathways is considered a priority for neglected diseases such as trypanosomiasis and leishmaniasis. The thiol-dependent redox metabolism of trypanosomatids relies on bis-glutathionylspermidine [trypanothione, T(SH)2], a low mole...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Benítez D,Medeiros A,Fiestas L,Panozzo-Zenere EA,Maiwald F,Prousis KC,Roussaki M,Calogeropoulou T,Detsi A,Jaeger T,Šarlauskas J,Peterlin Mašič L,Kunick C,Labadie GR,Flohé L,Comini MA

    更新日期:2016-04-12 00:00:00

  • Pediatric visceral leishmaniasis in Albania: a retrospective analysis of 1,210 consecutive hospitalized patients (1995-2009).

    abstract:BACKGROUND:Little information is available about infantile visceral leishmaniasis (VL) in Albania as regards incidence, diagnosis and management of the disease. METHODOLOGY/PRINCIPAL FINDINGS:Demographic data, clinical and laboratory features and therapeutic findings were considered in children admitted to University ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Petrela R,Kuneshka L,Foto E,Zavalani F,Gradoni L

    更新日期:2010-09-07 00:00:00

  • Bile Salts Modulate the Mucin-Activated Type VI Secretion System of Pandemic Vibrio cholerae.

    abstract::The causative agent of cholera, Vibrio cholerae, regulates its diverse virulence factors to thrive in the human small intestine and environmental reservoirs. Among this pathogen's arsenal of virulence factors is the tightly regulated type VI secretion system (T6SS). This system acts as an inverted bacteriophage to inj...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Bachmann V,Kostiuk B,Unterweger D,Diaz-Satizabal L,Ogg S,Pukatzki S

    更新日期:2015-08-28 00:00:00

  • The Global Economic and Health Burden of Human Hookworm Infection.

    abstract:BACKGROUND:Even though human hookworm infection is highly endemic in many countries throughout the world, its global economic and health impact is not well known. Without a better understanding of hookworm's economic burden worldwide, it is difficult for decision makers such as funders, policy makers, disease control o...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Bartsch SM,Hotez PJ,Asti L,Zapf KM,Bottazzi ME,Diemert DJ,Lee BY

    更新日期:2016-09-08 00:00:00

  • Response of extensively drug resistant Salmonella Typhi to treatment with meropenem and azithromycin, in Pakistan.

    abstract:INTRODUCTION:Salmonella Typhi is one of the leading health problems in Pakistan. With the emergence of extensively drug resistant (XDR) Salmonella Typhi, treatment options are limited. Here we report the clinical manifestations and the response to treatment of patients with XDR Typhoid fever. The patients were treated ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Qureshi S,Naveed AB,Yousafzai MT,Ahmad K,Ansari S,Lohana H,Mukhtar A,Qamar FN

    更新日期:2020-10-15 00:00:00

  • Identification of clinically approved small molecules that inhibit growth and affect transcript levels of developmentally regulated genes in the African trypanosome.

    abstract::Trypanosoma brucei are unicellular parasites endemic to Sub-Saharan Africa that cause fatal disease in humans and animals. Infection with these parasites is caused by the bite of the tsetse fly vector, and parasites living extracellularly in the blood of infected animals evade the host immune system through antigenic ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Walsh ME,Naudzius EM,Diaz SJ,Wismar TW,Martchenko Shilman M,Schulz D

    更新日期:2020-03-13 00:00:00

  • Caregivers' views on stigmatization and discrimination of people affected by leprosy in Ghana.

    abstract:BACKGROUND:Leprosy is a condition that has long been associated with stigma and discrimination, even when infected persons have been cured. This paper describes stigma and discrimination as viewed by caregivers who are associated with people affected by leprosy in Ghana. METHODS:A qualitative interview with semi-struc...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Asampong E,Dako-Gyeke M,Oduro R

    更新日期:2018-01-29 00:00:00

  • Knowledge, attitudes and practices towards yaws and yaws-like skin disease in Ghana.

    abstract:INTRODUCTION:Yaws is endemic in Ghana. The World Health Organization (WHO) has launched a new global eradication campaign based on total community mass treatment with azithromycin. Achieving high coverage of mass treatment will be fundamental to the success of this new strategy; coverage is dependent, in part, on appro...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Marks M,Kwakye-Maclean C,Doherty R,Adwere P,Aziz Abdulai A,Duah F,Ohene SA,Mitja O,Oguti B,Solomon AW,Mabey DCW,Adu-Sarkodie Y,Asiedu K,Ackumey MM

    更新日期:2017-07-31 00:00:00

  • MIRO and IRbase: IT tools for the epidemiological monitoring of insecticide resistance in mosquito disease vectors.

    abstract:BACKGROUND:Monitoring of insect vector populations with respect to their susceptibility to one or more insecticides is a crucial element of the strategies used for the control of arthropod-borne diseases. This management task can nowadays be achieved more efficiently when assisted by IT (Information Technology) tools, ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Dialynas E,Topalis P,Vontas J,Louis C

    更新日期:2009-06-23 00:00:00

  • Viral aetiology of central nervous system infections in adults admitted to a tertiary referral hospital in southern Vietnam over 12 years.

    abstract:BACKGROUND:Central nervous system (CNS) infections are important diseases in both children and adults worldwide. The spectrum of infections is broad, encompassing bacterial/aseptic meningitis and encephalitis. Viruses are regarded as the most common causes of encephalitis and aseptic meningitis. Better understanding of...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Tan le V,Thai le H,Phu NH,Nghia HD,Chuong LV,Sinh DX,Phong ND,Mai NT,Man DN,Hien VM,Vinh NT,Day J,Chau NV,Hien TT,Farrar J,de Jong MD,Thwaites G,van Doorn HR,Chau TT

    更新日期:2014-08-28 00:00:00

  • Isolation of Infective Zika Virus from Urine and Saliva of Patients in Brazil.

    abstract:BACKGROUND:Zika virus (ZIKV) is an emergent threat provoking a worldwide explosive outbreak. Since January 2015, 41 countries reported autochthonous cases. In Brazil, an increase in Guillain-Barré syndrome and microcephaly cases was linked to ZIKV infections. A recent report describing low experimental transmission eff...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Bonaldo MC,Ribeiro IP,Lima NS,Dos Santos AA,Menezes LS,da Cruz SO,de Mello IS,Furtado ND,de Moura EE,Damasceno L,da Silva KA,de Castro MG,Gerber AL,de Almeida LG,Lourenço-de-Oliveira R,Vasconcelos AT,Brasil P

    更新日期:2016-06-24 00:00:00

  • Presence of the knockdown resistance (kdr) mutations in the head lice (Pediculus humanus capitis) collected from primary school children of Thailand.

    abstract::Human head lice are blood-sucking insects causing an infestation in humans called pediculosis capitis. The infestation is more prevalent in the school-aged population. Scalp itching, a common presenting symptom, results in scratching and sleep disturbance. The condition can lead to social stigmatization which can lead...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Brownell N,Sunantaraporn S,Phadungsaksawasdi K,Seatamanoch N,Kongdachalert S,Phumee A,Siriyasatien P

    更新日期:2020-12-16 00:00:00

  • A 23-year retrospective investigation of Salmonella Typhi and Salmonella Paratyphi isolated in a tertiary Kathmandu hospital.

    abstract:BACKGROUND:Salmonella serovars Typhi (S. Typhi) and Paratyphi A (S. Paratyphi A), the causative agents of enteric fever, have been routinely isolated organisms from the blood of febrile patients in the Kathmandu Valley since the early 1990s. Susceptibility against commonly used antimicrobials for treating enteric fever...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Zellweger RM,Basnyat B,Shrestha P,Prajapati KG,Dongol S,Sharma PK,Koirala S,Darton TC,Dolecek C,Thompson CN,Thwaites GE,Baker SG,Karkey A

    更新日期:2017-11-27 00:00:00

  • Head and neck mycetoma: the mycetoma research centre experience.

    abstract::Mycetoma is a unique neglected tropical disease which is endemic in what is known as the "mycetoma belt". The disease has many devastating impacts on patients and communities in endemic area and is characterised by massive deformity, destruction and disability. Mycetoma is commonly seen in the foot and hand and less f...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Fahal A,Mahgoub el S,El Hassan AM,Jacoub AO,Hassan D

    更新日期:2015-03-13 00:00:00

  • Safety and efficacy of single dose versus multiple doses of AmBisome for treatment of visceral leishmaniasis in eastern Africa: a randomised trial.

    abstract:BACKGROUND:Anti-leishmanial drug regimens that include a single dose AmBisome could be suitable for eastern African patients with symptomatic visceral leishmaniasis (VL) but the appropriate single dose is unknown. METHODOLOGY:A multi-centre, open-label, non-inferiority, randomized controlled trial with an adaptive des...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Khalil EA,Weldegebreal T,Younis BM,Omollo R,Musa AM,Hailu W,Abuzaid AA,Dorlo TP,Hurissa Z,Yifru S,Haleke W,Smith PG,Ellis S,Balasegaram M,EL-Hassan AM,Schoone GJ,Wasunna M,Kimutai R,Edwards T,Hailu A

    更新日期:2014-01-16 00:00:00

  • An unhealing wound and subcutaneous nodules due to Sporothrix globosa after a cat bite.

    abstract::A 51-year-old man with 3-month unhealing cat bite wound was diagnosed with sporotrichosis, a subacute-to-chronic infection caused by the worldwide endemic, dimorphic fungus Sporothrix globosa. The case would help clinicians to raise awareness of human sporotrichosis due to cat bites, which remains rare and is likely t...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Liu Y,Liu L,Kang M,Zong Z

    更新日期:2020-12-03 00:00:00

  • Oxidative stress and DNA lesions: the role of 8-oxoguanine lesions in Trypanosoma cruzi cell viability.

    abstract::The main consequence of oxidative stress is the formation of DNA lesions, which can result in genomic instability and lead to cell death. Guanine is the base that is most susceptible to oxidation, due to its low redox potential, and 8-oxoguanine (8-oxoG) is the most common lesion. These characteristics make 8-oxoG a g...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Aguiar PH,Furtado C,Repolês BM,Ribeiro GA,Mendes IC,Peloso EF,Gadelha FR,Macedo AM,Franco GR,Pena SD,Teixeira SM,Vieira LQ,Guarneri AA,Andrade LO,Machado CR

    更新日期:2013-06-13 00:00:00

  • Unforeseen costs of cutting mosquito surveillance budgets.

    abstract::A budget proposal to stop the U.S. Centers for Disease Control and Prevention (CDC) funding in surveillance and research for mosquito-borne diseases such as dengue and West Nile virus has the potential to leave the country ill-prepared to handle new emerging diseases and manage existing ones. In order to demonstrate t...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Vazquez-Prokopec GM,Chaves LF,Ritchie SA,Davis J,Kitron U

    更新日期:2010-10-26 00:00:00

  • Diverse effects on mitochondrial and nuclear functions elicited by drugs and genetic knockdowns in bloodstream stage Trypanosoma brucei.

    abstract:BACKGROUND:The options for treating the fatal disease human African trypanosomiasis are limited to a few drugs that are toxic or facing increasing resistance. New drugs that kill the causative agents, subspecies of Trypanosoma brucei, are therefore urgently needed. Little is known about the cellular mechanisms that lea...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Worthen C,Jensen BC,Parsons M

    更新日期:2010-05-04 00:00:00