Abstract:
BACKGROUND:Aortic cross-clamping during abdominal aortic aneurysm (AAA) open repair leads to development of ischemia-reperfusion injury. Electron paramagnetic resonance spectroscopy (EPR) spin-trapping is a valuable method of direct measurement of free radicals. The objective of the study was to evaluate the results of EPR as a direct method of free radical measurement and degree of inflammatory response in open operative treatment of patients with AAA and aorto-iliac occlusive disease (AIOD). MATERIAL/METHODS:The study was performed on a group of 32 patients with AAA and 25 patients with AIOD scheduled for open repair. Peripheral venous blood for EPR spectroscopy and for SOD, GPx, ox-LDL, Il-6, TNF-alfa, CRP, and HO-1 were harvested. Selected parameters were established accordingly to specified EPR and immunohistochemical methods and analyzed between groups by Mann-Whitney U test and Wilcoxon matched-pairs signed-ranks test with Bonferroni correction. RESULTS:Free radicals level was correlated with the time of the aortic cross-clamping after the reperfusion of he first and second leg in AAA (r=0.7; r=0.47). ox-LDL in AAA decreased 5 min after reperfusion of the first leg (32.99 U/L, range: 14.09-77.12) and 5 min after reperfusion of the second leg (26.75 U/L, range: 11.56-82.12) and 24 h after the operation (25.85 U/L, range: 14.29-49.70). HO-1 concentration increased to above the level before intervention 24 h after surgery. The activities of GPx and SOD decreased 5 min after the first-leg reperfusion in AAA. Twenty-four hours after surgery, inflammatory markers increased in AAA to CRP was 14.76 ml/l (0.23-38.55), IL-6 was 141.22 pg/ml (84.3-591.03), TNF-alfa was 6.82 pg/ml (1.76-80.01) and AIOD: CRP was 18.44 mg/l (2.56-33.14), IL-6: 184.1 pg/ml (128.46-448.03), TNF-alfa was 7.74 pg/ml (1.74-74.74). CONCLUSIONS:EPR spin-trapping demonstrates temporarily elevated level of free radicals in early phase of reperfusion, leading to decrease antioxidants in AAA. Elevated free radical levels decreased 24 h after surgery due to various endogenous antioxidants and therapies.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Majewski W,Krzyminiewski R,Stanisić M,Iskra M,Krasiński Z,Nowak M,Dobosz Bdoi
10.12659/MSM.890774subject
Has Abstractpub_date
2014-11-27 00:00:00pages
2453-60eissn
1234-1010issn
1643-3750pii
890774journal_volume
20pub_type
杂志文章abstract::BACKGROUND This study investigated the prevalence and severity of low back pain (LBP), caesarean section (C-section) rate, and the anesthesia approaches among lumbar scoliosis patients undergoing anterior correction surgery, and compared them with a healthy control cohort. MATERIAL AND METHODS The inclusion criteria f...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.926960
更新日期:2020-10-17 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.898454
更新日期:2016-12-16 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.908762
更新日期:2018-01-30 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2009-11-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.906361
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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doi:
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2008-05-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,meta分析
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 临床试验,杂志文章
doi:
更新日期:2004-06-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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doi:
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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更新日期:2018-11-08 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
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更新日期:2017-08-16 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,meta分析,评审
doi:
更新日期:2009-03-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
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更新日期:2016-06-20 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
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更新日期:2015-12-09 00:00:00