Abstract：
:A primary goal of enamel research is to understand and potentially treat or prevent enamel defects related to amelogenesis imperfecta (AI). Rodents are ideal models to assist our understanding of how enamel is formed because they are easily genetically modified, and their continuously erupting incisors display all stages of enamel development and mineralization. While numerous methods have been developed to generate and analyze genetically modified rodent enamel, it is crucial to understand the limitations and challenges associated with these methods in order to draw appropriate conclusions that can be applied translationally, to AI patient care. We have highlighted methods involved in generating and analyzing rodent enamel and potential approaches to overcoming limitations of these methods: (1) generating transgenic, knockout, and knockin mouse models, and (2) analyzing rodent enamel mineral density and functional properties (structure and mechanics) of mature enamel. There is a need for a standardized workflow to analyze enamel phenotypes in rodent models so that investigators can compare data from different studies. These methods include analyses of gene and protein expression, developing enamel histology, enamel pigment, degree of mineralization, enamel structure, and mechanical properties. Standardization of these methods with regard to stage of enamel development and sample preparation is crucial, and ideally investigators can use correlative and complementary techniques with the understanding that developing mouse enamel is dynamic and complex.
journal_name
Front Physioljournal_title
Frontiers in physiologyauthors
Pugach MK,Gibson CWdoi
10.3389/fphys.2014.00313subject
Has Abstractpub_date
2014-09-17 00:00:00pages
313issn
1664-042Xjournal_volume
5pub_type
杂志文章,评审abstract：:Microbiopsies of human skeletal muscle are increasingly adopted by physiologists for a variety of experimental assays given the reduced invasiveness of this procedure compared to the classic Bergstrom percutaneous biopsy technique. However, a recent report demonstrated lower mitochondrial respiration in saponin-permea...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2015.00360
更新日期：2015-12-18 00:00:00
abstract：:The human brain is constantly active and even small limitations to cerebral blood flow (CBF) may be critical for preserving oxygen and substrate supply, e.g., during exercise and hypoxia. Exhaustive exercise evokes a competition for the supply of oxygenated blood between the brain and the working muscles, and inabilit...
journal_title：Frontiers in physiology
pub_type： 杂志文章,评审
doi：10.3389/fphys.2020.583155
更新日期：2021-01-15 00:00:00
abstract：:Prior peripheral hypoxia induced via remote ischemic preconditioning (IPC) can improve physical performance in male athletes through improved O2 delivery and utilization. Since females may have an innate protective mechanism against ischemia-reperfusion injury, and since muscle metabolism during contraction differs be...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2016.00674
更新日期：2017-01-05 00:00:00
abstract：:High-intensity interval training (HIT) is an effective approach for improving a range of physiological markers associated with physical fitness. A considerable body of work has demonstrated substantial improvements in cardiorespiratory fitness following short-term training programmes, while emerging evidence suggests ...
journal_title：Frontiers in physiology
pub_type： 杂志文章,评审
doi：10.3389/fphys.2019.00581
更新日期：2019-05-22 00:00:00
abstract：:Myocardial electrical impedance is a biophysical property of the heart that is influenced by the intrinsic structural characteristics of the tissue. Therefore, the structural derangements elicited in a chronic myocardial infarction should cause specific changes in the local systolic-diastolic myocardial impedance, but...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2016.00389
更新日期：2016-08-31 00:00:00
abstract：:The RR-interval time series or tachograms obtained from electrocardiograms have been widely studied since they reflect the cardiac variability, and this is an indicative of the health status of a person. The tachogram can be seen as a highly non-linear and complex time series, and therefore, should be analyzed with no...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2020.00981
更新日期：2020-08-12 00:00:00
abstract：:Muscle and tendon stiffness are related to sports performance, tendinopathy, and tendon degeneration. However, the effects of habitual loading on muscle and tendon mechanical properties are unclear. Using amateur basketball players as examples, we investigated the effects of mechanical loading on the stiffness of the ...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2020.606706
更新日期：2020-12-10 00:00:00
abstract：Objective:Varicose veins are a common problem worldwide and can cause significant impairments in health-related quality of life, but the etiology and pathogenesis remain not well defined. This study aims to elucidate transcriptomic regulations of varicose veins by detecting differentially expressed genes, pathways and ...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2019.00278
更新日期：2019-03-19 00:00:00
abstract：:Aim: mircoRNAs (miRNAs), small non-coding RNAs regulating gene expression, are stably secreted into the blood and circulating miRNAs (c-miRNAs) may play an important role in cell-cell communication. Furthermore, c-miRNAs might serve as novel biomarkers of the current vascular cell status. Here, we examined how the lev...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2016.00643
更新日期：2016-12-26 00:00:00
abstract：:Eccentric loading is an effective stimulus for muscle hypertrophy and strength gains, however, the effect of lengthening velocity is under debate. The purpose of the current study was to investigate the influence of muscle lengthening velocity during eccentric training on muscle hypertrophy and strength gains at a giv...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2019.00957
更新日期：2019-07-31 00:00:00
abstract：:Purpose: Blood biomarkers are a useful tool to study concussion. However, their interpretation is complicated by a number of potential biological confounds, including exercise. This is particularly relevant in military and athletic settings where injury commonly occurs during physical exertion. The impact of high-inte...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2018.01367
更新日期：2018-09-28 00:00:00
abstract：:Planarians undergo continuous body size remodeling under starvation or during regeneration. This process likely involves autophagy and autophagic cell death, but this hypothesis is supported by few studies. To test this hypothesis, we cloned and characterized autophagy-related gene 7 (Atg7) from the planarian Dugesia ...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2018.01223
更新日期：2018-09-04 00:00:00
abstract：:The effect of training status on post-exercise flow-mediated dilation (FMD) is not well characterized. We tested the hypothesis that the more trained the subjects, the lower the reduction in FMD after an acute bout of aerobic exercise. Forty-seven men (mean ± SD, age: 20.1 ± 1.2 years, body mass: 75.5 ± 5.1 kg, height...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2020.00348
更新日期：2020-05-08 00:00:00
abstract：:Carbonic anhydrase II (CAII) is expressed along the nephron where it interacts with a number of transport proteins augmenting their activity. Aquaporin-1 (AQP1) interacts with CAII to increase water flux through the water channel. Both CAII and aquaporin-1 are expressed in the thin descending limb (TDL); however, the ...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2017.01108
更新日期：2018-01-05 00:00:00
abstract：:Tubular aggregates (TAs) in skeletal muscle fibers are unusual accumulation of sarcoplasmic reticulum (SR) tubes that are found in different disorders including TA myopathy (TAM). TAM is a muscular disease characterized by muscle pain, cramping, and weakness that has been recently linked to mutations in STIM1 and ORAI...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2020.601057
更新日期：2021-01-05 00:00:00
abstract：:Background: Subthalamic Nucleus Deep Brain Stimulation (STN-DBS) is highly effective in alleviating motor symptoms of Parkinson's disease (PD) which are not optimally controlled by dopamine replacement therapy. Clinical studies and reports suggest that STN-DBS may result in increased impulsivity and de novo impulse co...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2016.00585
更新日期：2016-11-29 00:00:00
abstract：:Excitatory and inhibitory junction potentials of circular smooth muscle cells in guinea pig ileum and colon are suppressed 30-90 min after setting up in vitro preparations. We have previously shown this "unresponsive" period is associated with a transient loss of dye coupling between smooth muscle cells, which subsequ...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2014.00319
更新日期：2014-08-22 00:00:00
abstract：:Extracellular vesicles (EVs) - including exosomes, microvesicles and apoptotic bodies - have received much scientific attention last decade as mediators of a newly discovered cell-to-cell communication system, acting at short and long distances. EVs carry biologically active molecules, thus providing signals that infl...
journal_title：Frontiers in physiology
pub_type： 杂志文章,评审
doi：10.3389/fphys.2018.00651
更新日期：2018-06-05 00:00:00
abstract：Introduction:MTHFR methylation status is associated with microvascular complications in diabetes, but the factors influencing this profile remain unknown. Objective:The aim of this study was to evaluate the influence of physical activity level and nutritional status on the methylation profile of the MTHFR gene in pati...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2020.618672
更新日期：2021-01-12 00:00:00
abstract：Introduction:Baroreflex sensitivity (BRS) is essential to ensure rapid adjustment to variations in blood pressure (BP). Spontaneous baroreflex function can be assessed using continuous recordings of blood pressure. The goal of this study was to compare four methods for BRS quantification [the sequence, Bernardi's (BER)...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2019.01505
更新日期：2019-12-10 00:00:00
abstract：:[This corrects the article on p. 165 in vol. 7, PMID: 27242539.]. ...
journal_title：Frontiers in physiology
pub_type： 已发布勘误
doi：10.3389/fphys.2017.00568
更新日期：2017-08-11 00:00:00
abstract：:Temporal computations are important in the acoustic communication of anurans. In many cases, calls between closely related species are nearly identical spectrally but differ markedly in temporal structure. Depending on the species, calls can differ in pulse duration, shape and/or rate (i.e., amplitude modulation), dir...
journal_title：Frontiers in physiology
pub_type： 杂志文章,评审
doi：10.3389/fphys.2014.00206
更新日期：2014-05-30 00:00:00
abstract：:Heart Failure (HF) is associated with significantly high morbidity and mortality. We performed a meta-analysis and updated new evidences from randomized controlled trials (RCTs) to determine the effects of Tai Chi (TC) in patients with HF. Electronic literature search of Medline, PubMed, EMBASE, the Cochrane Library, ...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2017.00989
更新日期：2017-12-07 00:00:00
abstract：:Objective: Neuromuscular injury prevention programs (IPP) can reduce injury rate by about 40% in youth sport. Multimodal IPP include, for instance, balance, strength, power, and agility exercises. Our systematic review and meta-analysis aimed to evaluate the effects of multimodal IPP on neuromuscular performance in yo...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2017.00791
更新日期：2017-10-12 00:00:00
abstract：:As verbal encouragement (VE) is used in high intensity functional exercise testing, this randomized controlled crossover study aimed at investigating whether VE affects high intensity functional strength and endurance performance testing. We further examined between-day variability of high intensity functional strengt...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2019.00460
更新日期：2019-04-25 00:00:00
abstract：:We explored the metagenomic, metabolomic and trace metal makeup of intestinal microbiota and environment in healthy male participants during the run-in (5 day) and the following three 21-day interventions: normoxic bedrest (NBR), hypoxic bedrest (HBR) and hypoxic ambulation (HAmb) which were carried out within a contr...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2018.00198
更新日期：2018-03-13 00:00:00
abstract：:Periventricular-intraventricular hemorrhages (PIVH) and (diffuse) white matter injury (WMI) are the most important acquired brain lesions of the very and extremely prematurely born neonate. Both carry a high risk for death or adverse neurodevelopmental outcome. The first part of the review discusses the standard of ca...
journal_title：Frontiers in physiology
pub_type： 杂志文章,评审
doi：10.3389/fphys.2019.00181
更新日期：2019-03-20 00:00:00
abstract：:Accurate evaluation of the appearance of QTc sex differences during childhood and adolescence is intricate. Inter-subject differences of individual QT/RR patterns make generic heart rate corrections inaccurate because of fast resting heart rates in children. The study investigated 527 healthy children and adolescents ...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2019.00994
更新日期：2019-08-02 00:00:00
abstract：:Social attachment formed by filial imprinting in newborn chicks undergoes a process of memory consolidation that involves rearrangement of its neural storage substrates. In the first 3 h after imprinting it depends on the integrity of the intermediate medial mesopallium (IMM) and beyond that time on unidentified memor...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2019.00351
更新日期：2019-03-29 00:00:00
abstract：:Recently, we reported that Dahl salt-sensitive leptin receptor mutant (SSLepRmutant) rats exhibit dyslipidemia and renal lipid accumulation independent of hyperglycemia that progresses to chronic kidney disease (CKD). Therefore, in the current study, we examined the effects of gemfibrozil, a lipid-lowering drug (200 m...
journal_title：Frontiers in physiology
pub_type： 杂志文章
doi：10.3389/fphys.2020.566403
更新日期：2020-09-18 00:00:00
