Leptin decreases apoptosis and alters BCL-2 : Bax ratio in clonal rodent pancreatic beta-cells.

Abstract:

AIMS/HYPOTHESIS:The adipocyte derived peptide hormone leptin is known to regulate apoptosis and cell viability in several cells and tissues, as well as having several pancreatic islet beta-cell specific effects such as inhibition of glucose-stimulated insulin secretion. This study investigated the effects of leptin upon apoptosis induced by serum depletion and on expression of the apoptotic regulators B-cell leukaemia 2 gene product (BCL-2) and BCL2-associated X protein (Bax) in the glucose-responsive BRIN-BD11 beta-cell line. METHODS:BRIN-BD11 cells were cultured in RPMI 1640 and subsequently serum depleted +/- leptin (10 and 50 ng/mL) for 24 h. Cell viability and apoptosis were measured using a modified MTS assay and TUNEL/YO-PRO-1 assays, respectively. BCL-2 and Bax expression were measured by real-time PCR and Western blotting. RESULTS:Leptin caused a reduction in serum-depleted apoptosis, although it failed to have any effect on the overall cell viability, causing a 68% shift from apoptosis to necrosis. Leptin significantly increased the level of BCL-2 mRNA expression (150% compared to serum depletion alone), without altering Bax mRNA expression. At the protein level, leptin increased BCL-2 and decreased Bax, altering the BCL-2 : Bax ratio. CONCLUSIONS:We conclude that leptin reduces apoptosis in beta-cells at physiological concentrations, possibly via its ability to up-regulate BCL-2 and Bax expression.

journal_name

Diabetes Metab Res Rev

authors

Brown JE,Dunmore SJ

doi

10.1002/dmrr.726

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

497-502

issue

6

eissn

1520-7552

issn

1520-7560

journal_volume

23

pub_type

杂志文章
  • Oestradiol levels may differ between premenopausal women, ages 18-50, with type 1 diabetes and matched controls.

    abstract:BACKGROUND:This study aimed to investigate whether oestradiol differs between premenopausal women with and without type 1 diabetes and whether levels are associated with such factors as age, reproductive history or diabetes management. METHODS:Oestradiol in premenopausal women with type 1 diabetes (n = 89; age = 18-50...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.2829

    authors: Hassan LS,Monson RS,Danielson KK

    更新日期:2017-02-01 00:00:00

  • A systematic review of the effectiveness of revascularization of the ulcerated foot in patients with diabetes and peripheral arterial disease.

    abstract::In several large recent observational studies, peripheral arterial disease (PAD) was present in up to 50% of the patients with a diabetic foot ulcer and was an independent risk factor for amputation. The International Working Group on the Diabetic Foot therefore established a multidisciplinary working group to evaluat...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审

    doi:10.1002/dmrr.2249

    authors: Hinchliffe RJ,Andros G,Apelqvist J,Bakker K,Friederichs S,Lammer J,Lepantalo M,Mills JL,Reekers J,Shearman CP,Valk G,Zierler RE,Schaper NC

    更新日期:2012-02-01 00:00:00

  • Increased LDL cholesterol and CRP in infants of mothers with type 1 diabetes.

    abstract:BACKGROUND:Proatherogenic stimuli during foetal life may predispose to development of atherosclerosis in adulthood. Elevated plasma low-density lipoprotein (LDL) cholesterol and C-reactive protein (CRP) expression is associated with increased risk of atherosclerosis. METHODS AND RESULTS:In this study, we examined how ...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.867

    authors: Lindegaard ML,Svarrer EM,Damm P,Mathiesen ER,Nielsen LB

    更新日期:2008-09-01 00:00:00

  • Ceftaroline fosamil for treatment of diabetic foot infections: the CAPTURE study experience.

    abstract:BACKGROUND:To ascertain which demographic, clinical, and microbiological factors might affect clinical outcomes of patients with diabetic foot infections, excluding known osteomyelitis, by analysing Clinical Assessment Program and Teflaro® Utilization Registry study data of patients treated with ceftaroline fosamil. M...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,多中心研究

    doi:10.1002/dmrr.2624

    authors: Lipsky BA,Cannon CM,Ramani A,Jandourek A,Calmaggi A,Friedland HD,Goldstein EJ

    更新日期:2015-05-01 00:00:00

  • Role of previous treatment with sulfonylureas in diabetic patients with acute myocardial infarction: results from a nationwide French registry.

    abstract:BACKGROUND:The cardiovascular effects of sulfonylureas (SU) in diabetic patients are controversial and it has been suggested that diabetic patients with acute myocardial infarction while on SU were at increased risk. OBJECTIVES:To assess the in-hospital outcome of patients with acute myocardial infarction according to...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,多中心研究

    doi:10.1002/dmrr.498

    authors: Danchin N,Charpentier G,Ledru F,Vaur L,Guéret P,Hanania G,Blanchard D,Lablanche JM,Genès N,Cambou JP

    更新日期:2005-03-01 00:00:00

  • Prevalence of early and late prematurity is similar among pediatric type 1 diabetes patients and the general population.

    abstract:BACKGROUND:The incidence of type 1 diabetes mellitus (T1DM) has increased in recent decades, as has the incidence of preterm births (<37 weeks). We aimed to evaluate and compare the prevalence of prematurity and early prematurity (<34 weeks) and birth season variability among T1DM and non-T1DM children. METHODS:A nati...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.2996

    authors: Adar A,Shalitin S,Eyal O,Loewenthal N,Pinhas-Hamiel O,Levy M,Dally-Gottfried O,Landau Z,Zung A,Levy-Khademi F,Zangen D,Tenenbaum-Rakover Y,Rachmiel M

    更新日期:2018-07-01 00:00:00

  • Thiazolidinediones as anti-inflammatory and anti-atherogenic agents.

    abstract::In the last few years, there has been increasing focus on the impact of interventions on cardiovascular outcomes in patients with type 2 diabetes. Insulin resistance and hyperglycaemia often co-exist with a cluster of risk factors for coronary artery disease, but the underlying mechanisms leading to the development of...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审

    doi:10.1002/dmrr.790

    authors: Ceriello A

    更新日期:2008-01-01 00:00:00

  • Blood group AB is protective factor for gestational diabetes mellitus: a prospective population-based study in Tianjin, China.

    abstract:BACKGROUND:The ABO blood types are associated with cancers, cardiovascular diseases and type 2 diabetes mellitus but whether they are also associated with gestational diabetes mellitus (GDM) is unknown. We examined the relationship between the ABO blood types and the risk of GDM in a prospective population-based Chines...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.2650

    authors: Zhang C,Li Y,Wang L,Sun S,Liu G,Leng J,Guo J,Lv L,Li W,Zhang C,Hu G,Yu Z,Yang X

    更新日期:2015-09-01 00:00:00

  • Cardiovascular disease in patients with type 1 diabetes: Early evaluation, risk factors and possible relation with cardiac autoimmunity.

    abstract::Cardiovascular disease now is the leading cause of mortality among patients with type 1 diabetes (T1D). The risk of death from cardiovascular events in subjects with T1D is 2-10 times higher than the general population, depending on blood glucose control. Although complications of cardiovascular disease occur in middl...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审

    doi:10.1002/dmrr.3423

    authors: Shi M,Tang R,Huang F,Zhong T,Chen Y,Li X,Zhou Z

    更新日期:2020-11-30 00:00:00

  • Can we prevent beta cell apoptosis in type 2 diabetes?

    abstract::Since the publication of the United Kingdom Prospective Diabetes Study (UKPDS), the progressive nature of type 2 (DM2) diabetes has been identified as the main cause of failure to maintain a long term glycemic control.[1] The need to adjust treatment as the disease progressed was recognized and algorithms for treatmen...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.2381

    authors: Eliaschewitz FG,Tambascia MA

    更新日期:2012-12-05 00:00:00

  • Induction of long-term normoglycemia without medication in Korean type 2 diabetes patients after continuous subcutaneous insulin infusion therapy.

    abstract:BACKGROUND:Our previous studies showed that some Korean type 2 diabetic patients could maintain long-term normal blood glucose control without any medication, referred to as remission after a certain period of normalization of blood glucose level by continuous subcutaneous insulin infusion (CSII) treatment. In this stu...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.343

    authors: Park S,Choi SB

    更新日期:2003-03-01 00:00:00

  • Influence of diabetic metabolic state on fracture healing in spontaneously diabetic rats.

    abstract:BACKGROUND:Type 1 diabetes mellitus (DM) has been shown to alter the properties of bone and impair fracture healing in both humans and animals. The objective of this study was to examine changes in the histomorphometrical, histological and mechanical parameters of bone and remodeling during fracture healing, depending ...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.537

    authors: Follak N,Klöting I,Merk H

    更新日期:2005-05-01 00:00:00

  • Economic aspects of diabetic foot care in a multidisciplinary setting: a review.

    abstract:BACKGROUND:To evaluate the economic aspects of diabetic foot care in a multidisciplinary setting. METHOD:A review of the English language literature, published from 1966 to November 2005. RESULTS:The results of available studies on the cost-of-illness of diabetic foot problems are difficult to compare. Nevertheless t...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审

    doi:10.1002/dmrr.706

    authors: Matricali GA,Dereymaeker G,Muls E,Flour M,Mathieu C

    更新日期:2007-07-01 00:00:00

  • Angiotensin-converting enzyme 2 inhibits endoplasmic reticulum stress-associated pathway to preserve nonalcoholic fatty liver disease.

    abstract:BACKGROUND:Previous works indicated that the stress on the endoplasmic reticulum (ER) affected nonalcoholic fatty liver disease (NAFLD). However, there is no clear evident on the effect of the regulation of ER stress by angiotensin-converting enzyme 2 (ACE2) on the prevention of NAFLD. METHODS:HepG2 cells were treated...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.3123

    authors: Cao X,Song LN,Zhang YC,Li Q,Shi TT,Yang FY,Yuan MX,Xin Z,Yang JK

    更新日期:2019-05-01 00:00:00

  • Prevention of type 2 diabetes mellitus: is it feasible?

    abstract::The increasing global prevalence of type 2 diabetes mellitus (T2DM) requires the implementation of preventive strategies to halt this trend, tailored to the specific needs of individual regions. Risk factors for T2DM are among the main targets for improving health outcomes and curbing the development of diabetes; exce...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审

    doi:10.1002/dmrr.2513

    authors: Palermo A,Maggi D,Maurizi AR,Pozzilli P,Buzzetti R

    更新日期:2014-03-01 00:00:00

  • The development of a World Health Organisation international standard for islet cell antibodies: the aims and design of an international collaborative study.

    abstract::Islet cell antibodies (ICA) are a specific marker for Type 1 (insulin-dependent) diabetes mellitus. ICA are found in the serum of over 80% of newly diagnosed patients and the levels of ICA are directly of prognostic value. Standardisation of ICA and the uniform reporting of ICA levels in international units is critica...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/(sici)1520-7560(199901/02)15:1<72::aid-dmr

    authors: Mire-Sluis AR,Das RG,Lernmark A

    更新日期:1999-01-01 00:00:00

  • Preventing diabetes in women with gestational diabetes.

    abstract::The immediate consequences of gestational diabetes on pregnancy are well known but the complications decades later for the mother and child are just now emerging. This trio of papers discuss the long-term consequences of gestational diabetes, the importance of screening this high risk group of women for type 2 diabete...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 社论

    doi:10.1002/dmrr.2280

    authors: Feig D

    更新日期:2012-05-01 00:00:00

  • Association of serum 25-hydroxyvitamin D with type 2 diabetes and markers of insulin resistance in a general older population in Finland.

    abstract:BACKGROUND:Vitamin D insufficiency and type 2 diabetes are both common in Finland, and low vitamin D status has been suggested as a risk factor for type 2 diabetes. Our aim was to study the associations between serum 25-hydroxyvitamin D [25(OH)D], a marker of vitamin D status, and glucose homeostasis and type 2 diabete...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.2286

    authors: Hurskainen AR,Virtanen JK,Tuomainen TP,Nurmi T,Voutilainen S

    更新日期:2012-07-01 00:00:00

  • Early transcriptional regulation by C-peptide in freshly isolated rat proximal tubular cells.

    abstract:BACKGROUND:Clinical studies have shown that proinsulin C-peptide exerts renoprotective effects in type 1 diabetes, although the underlying mechanisms are poorly understood. As C-peptide has been shown to induce several intracellular events and to localize to nuclei, we aimed to determine whether gene transcription is a...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.1220

    authors: Lindahl E,Nordquist L,Müller P,El Agha E,Friederich M,Dahlman-Wright K,Palm F,Jörnvall H

    更新日期:2011-10-01 00:00:00

  • Skeletal involvement in patients with diabetes mellitus.

    abstract::Studies on skeletal involvement in patients with diabetes mellitus have generated conflicting results, largely because of the pathogenetic complexity of the condition. Several mechanisms may contribute to skeletal damage, including the increased urinary excretion coupled with the lower intestinal absorption of calcium...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审

    doi:10.1002/dmrr.449

    authors: Carnevale V,Romagnoli E,D'Erasmo E

    更新日期:2004-05-01 00:00:00

  • Implementation of a quality improvement initiative in Belgian diabetic foot clinics: feasibility and initial results.

    abstract:BACKGROUND:This article aims to describe the implementation and initial results of an audit-feedback quality improvement initiative in Belgian diabetic foot clinics. METHODS:Using self-developed software and questionnaires, diabetic foot clinics collected data in 2005, 2008 and 2011, covering characteristics, history ...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.2524

    authors: Doggen K,Van Acker K,Beele H,Dumont I,Félix P,Lauwers P,Lavens A,Matricali GA,Randon C,Weber E,Van Casteren V,Nobels F,Initiative for Quality Improvement and Epidemiology in Diabetic Foot Clinics (IQED-Foot) Study Group.

    更新日期:2014-07-01 00:00:00

  • Outcomes of hyperglycemia in patients with and without diabetes hospitalized for infectious diseases.

    abstract:OBJECTIVE:To examine the prognostic implications of diabetes mellitus (DM) and the importance of glycemic control during hospitalization for infectious diseases. METHODS:Historical prospectively collected data of patients hospitalized between 2011 and 2013. Infection-related hospitalizations were classified according ...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.3027

    authors: Akirov A,Diker-Cohen T,Masri-Iraqi H,Duskin-Bitan H,Shimon I,Gorshtein A

    更新日期:2018-10-01 00:00:00

  • Diabetic foot ulcer classification system for research purposes: a progress report on criteria for including patients in research studies.

    abstract::Various different systems have been proposed to classify diabetic foot ulcers, but none has gained widespread acceptance. The International Working Group of the Diabetic Foot (IWGDF) developed a classification system for research purposes, which is described in this report. In this PEDIS system, all foot ulcers should...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审

    doi:10.1002/dmrr.464

    authors: Schaper NC

    更新日期:2004-05-01 00:00:00

  • A case-control study: The association of serum paraoxonase 1 activity and concentration with the development of type 2 diabetes mellitus.

    abstract:BACKGROUND:A longitudinal study assessed serum paraoxonase 1 (PON1) activity and concentration as affected by age and as associated with the development of type 2 diabetes (T2D). PON1's recently established physiological function is the hydrolysis of lipolactones in oxidized LDL particles. METHODS:Serum samples and cl...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.2967

    authors: Crow JA,Meek EC,Wills RW,Chambers JE

    更新日期:2018-03-01 00:00:00

  • Negative T cell costimulation and islet tolerance.

    abstract::Activation of self-reactive T cells that specifically destroy the pancreatic beta-cells is one of the hallmarks in the development of type 1 diabetes. Thus, for prevention and treatment of this autoimmune disease, approaches to induce and maintain T cell tolerance toward the beta-cells, especially in islet transplanta...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审

    doi:10.1002/dmrr.345

    authors: Gao W,Demirci G,Li XC

    更新日期:2003-05-01 00:00:00

  • Stability of mitochondrial DNA against reactive oxygen species (ROS) generated in diabetes.

    abstract:BACKGROUND:Increased production of reactive oxygen species (ROS) in mitochondria has been proposed as the pathogenic mechanism for chronic complications of diabetes. Mitochondrial DNA (mtDNA) is more vulnerable to reactive oxygen species. However, there are few data on the mitochondrial DNA damage in diabetes and these...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.1203

    authors: Savu O,Sunkari VG,Botusan IR,Grünler J,Nikoshkov A,Catrina SB

    更新日期:2011-07-01 00:00:00

  • Risk factors in V-shaped risk associations with all-cause mortality in type 2 diabetes-The Hong Kong Diabetes Registry.

    abstract:BACKGROUND:Body mass index (BMI) is associated with death in a V-shaped manner in general populations but it is unknown whether BMI or other risk factors also exhibit V-shaped relationships with death in type 2 diabetic patients. METHODS:A prospective cohort of 7534 Chinese, type 2 diabetic patients enrolled since 199...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.792

    authors: So WY,Yang X,Ma RC,Kong AP,Lam CW,Ho CS,Cockram CS,Ko GT,Chow CC,Wong V,Tong PC,Chan JC

    更新日期:2008-03-01 00:00:00

  • Continuous glucose monitoring: current clinical use.

    abstract::Four kinds of subcutaneous continuous glucose monitoring (CGM) machines have been currently introduced in clinical practice. These machines exhibit real-time glucose on the monitor every 5 minutes and have alarms to indicate hypoglycaemia and hyperglycaemia. However, thus far, there is no clear consensus about the cli...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审

    doi:10.1002/dmrr.2346

    authors: Kim HS,Shin JA,Chang JS,Cho JH,Son HY,Yoon KH

    更新日期:2012-12-01 00:00:00

  • An increase in inflammation and islet dysfunction is a feature of prediabetes.

    abstract:AIMS:Type 2 diabetes (T2D) is a global health problem that will be diagnosed in almost 300 million people by 2025 according to the World Health Organization. Before being diagnosed with T2D, individuals may have glucose levels above normal but below the diabetic range. This condition is known as prediabetes. Studies sh...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.3405

    authors: Weaver JR,Odanga JJ,Breathwaite EK,Treadwell ML,Murchinson AC,Walters G,Fuentes DP,Lee JB

    更新日期:2020-09-10 00:00:00

  • Predicting ulcer-free survival using the discriminative value of screening test locations.

    abstract:BACKGROUND:Current guidelines recommend screening the feet of diabetic subjects with a 10-g monofilament or tuning fork. We investigated which tests and locations on the feet have the best predictive value regarding 1-year ulcer-free survival in diabetic subjects participating in the prospective Rotterdam Diabetic Foot...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章

    doi:10.1002/dmrr.3119

    authors: Rinkel WD,van der Oest MJW,Dijkstra DA,Castro Cabezas M,Coert JH

    更新日期:2019-03-01 00:00:00