Abstract:
OBJECTIVES:The Head and Neck Squamous Cell Carcinoma (HNSCC) ranks sixth worldwide. The mechanisms of growth, invasion and metastasis of this pathology are extensively studied and generally related to specific variations in signaling pathways like the PI3K-Akt; however most of these competent studies have been performed bidimensionally, which may hide important questions. This study sought to analyze the influence of the microenvironment upon the behavior of HNSCC. STUDY DESIGN:The status of pAkt, NF-κB and Cyclin D1 proteins was accessed through immunofluorescence and western blot methods in HNSCC cell lines originating from tongue, pharynx and metastatic lymph node when submitted to a three-dimensional culture model utilizing a matrix system. A bidimensional culture model (monolayer) was used as control. RESULTS:The HNSCC cell lines cultured three-dimensionally exhibited a growth pattern characterized by small isolated islands, different from the control group. When the three-dimensional model was applied, two of the studied cell lines showed the same expression pattern as the bidimensional model regarding nuclear or cytoplasmatic localization, as well as reduction of all protein levels; however, the cell line originated from tongue, which specially has the epidermal growth factor receptor constitutively activated, demonstrated nuclear translocation of pAkt and also an increase in the levels of Cyclin D1. CONCLUSIONS:The results suggest the influence of the microenvironment upon the behavior of HNSCC cells due to the changed expression of proteins related to tumor growth and cellular invasion. Furthermore, intrinsically genetic conditions also played important roles over the cells, despite the culture model employed.
journal_name
Med Oral Patol Oral Cir Bucaljournal_title
Medicina oral, patologia oral y cirugia bucalauthors
Salgueiredo-Giudice F,Corrêa-Abrahão A,Fornias-Sperandio F,da-Costa-Dal-Vechio AM,dos-Santos-Pinto-Junior Ddoi
10.4317/medoral.17538subject
Has Abstractpub_date
2012-05-01 00:00:00pages
e377-82issue
3eissn
1698-4447issn
1698-6946pii
17538journal_volume
17pub_type
杂志文章abstract:BACKGROUND:Mandibular sagittal split osteotomy (MSSO) may incur unfavorable split and sensorineural injuries. Knowledge of the anatomic location of the mandibular canal (MC) and bone thickness in the region of interest for MSSO, and the possible variations by age and gender can assist in avoiding such complications. Pu...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.22969
更新日期:2019-07-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to determine the prevalence of level IIb metastasis in patients with oral squamous cell carcinomas (OSCCs). MATERIAL AND METHODS:A prospective analysis of 56 patients with OSCC who underwent surgical treatment of the primary lesion with simultaneous neck dissection was performe...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.20491
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVES:To determine the incidence, severity and duration of lingual tactile and gustatory function impairments after lower third molar removal. STUDY DESIGN:Prospective cohort study with intra-subject measures of 16 patients undergoing lower third molar extractions. Sensibility and gustatory functions were evaluat...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.17890
更新日期:2012-09-01 00:00:00
abstract:OBJECTIVES:The principle of the intraosseous anesthesia (IOA) relies on the perforation of the cortical plate of the bone for direct application of the local anesthetic solution into the underlying cancellous structures. During this procedure, IOA needles might accidentally come in contact with the tooth roots. The aim...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.18386
更新日期:2013-01-01 00:00:00
abstract::Angioleiomyoma is an uncommon benign soft tissue tumor usually found in the lower extremities and rarely observed in oral tissues. It is microscopically characterized as a proliferation of smooth muscle cells intermingled with abundant vascular channels. Oral angioleiomyomas affect mostly the lips, palate, buccal muco...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章,评审
doi:10.4317/medoral.16.e482
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVES:We attempt to investigate a possible association between periodontal disease (PD) and mental disorders (MD) in a population of Brazilian Police. STUDY METHOD:From a total study population consisting of 803 policemen, 345 police officers were obtained by a sample calculation using the finite population corre...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:
更新日期:2011-09-01 00:00:00
abstract:INTRODUCTION:Frey's Syndrome is defined by facial hyperhidrosis in the preauricular region unleashed by gustatory stimulus and caused mainly by parotidectomy. Several treatment and prevention measures have been proposed, with no conclusive results. Recently, injections of Botulinum Toxin have been suggested, obtaining ...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:
更新日期:2008-04-01 00:00:00
abstract::Tooth agenesis is one of the most common congenital malformations in humans. Hypodontia can either occur as an isolated condition (non-syndromic hypodontia) or can be associated with a syndrome (syndromic hypodontia), highlighting the heterogeneity of the condition. Though much progress has been made to identify the d...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章,评审
doi:10.4317/medoral.19158
更新日期:2014-03-01 00:00:00
abstract:OBJECTIVE:To present 57 cases of oral ranula in children, analyzing the clinical characteristics, treatment and outcome of these lesions. METHODS:The clinical histories of patients diagnosed with oral ranula, seen between 1998 and 2008 at the Oral and Maxillofacial Surgery Unit of a reference Children 's Hospital (0-1...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.16.e158
更新日期:2011-03-01 00:00:00
abstract::Pericoronitis is an infectious disease often associated with the eruption of a third molar. It can be either acute (serous and suppurative) or chronic. Pain is usually the predominant symptom in acute stages, whereas chronic forms of the disease may display very few symptoms. Both present exudate. The infection is mul...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::External apical root resorption is a common phenomenon associated with orthodontic treatment. The factors relevant to root resorption can be divided into biological and mechanical factors. Some mechanical and biological factors might be associated with an increased or decreased risk of root resorption during orthodont...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章,评审
doi:
更新日期:2007-12-01 00:00:00
abstract:AIMS:To identify factors related to advanced-stage diagnosis of oral cancer to disclose high-risk groups and facilitate early detection strategies. STUDY DESIGN:An ambispective cohort study on 88 consecutive patients treated from January 1998 to December 2003. INCLUSION CRITERIA:pathological diagnosis of OSCC (primar...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.17399
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:The purpose of the present study was to investigate the expression of the α2-integrin subunit and heat shock protein 47 (Hsp47) in two families with isolated gingival fibromatosis (GF) form and one family with GF associated with dental abnormalities and normal gingival (NG). STUDY DESIGN:Immunohistochemistry...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.17970
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:The literature does not report any association between habitual snoring and sleep bruxism, but these situations can be a reason for frequent complaints of individuals, impairing the quality of life. This study was performed to investigate the sleep period and amount of habitual snoring in individuals with sl...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.23136
更新日期:2019-11-01 00:00:00
abstract::Recently there have been reports of osteonecrosis of the jaw (ONJ) in patients with chronic therapy with bisphosphonates (Bps). So far three drugs have been linked: Pamidronate of disodium, Zoledronic acid and Alendronate of sodium. It is due to a non detected side effect in clinical trials before commercialization, a...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:
更新日期:2007-08-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to compare the effects in terms of resistance to fracture of the mandibular condyle and femoral head following different doses of zoledronic acid in an animal model. STUDY DESIGN:A total of 80 adult male Sprague-Dawley rats were included in a prospective randomized study. The animal...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.18449
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND:The aim of this review is to systematically investigate the effect of a susceptible genotype to periodontitis with the clinical outcomes of periodontal regeneration. MATERIAL AND METHODS:Based on a focused question, an electronic search identified 155 unique citations. Three journals (Journal of Periodontol...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章,评审
doi:10.4317/medoral.21105
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVES:After maxillectomy, prosthetic restoration of the resulting defect is an essential step because it signals the beginning of patient's rehabilitation. The obturator used to restore the defect should be comfortable, restore adequate speech, deglutition, mastication, and be cosmetically acceptable, success will...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.17482
更新日期:2012-03-01 00:00:00
abstract::Osteochondroma is the most common benign neoplasia of the skeleton. In the head it was been described in cranial base, posterior maxillary surface, maxillary sinus, and different mandibular areas like condyle, ramus, body and symphysis. Osteochondroma of the coronoid process are rare. We present a review of the litera...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章,评审
doi:
更新日期:2006-05-01 00:00:00
abstract:PURPOSE:The purpose of the article is to present 8 new cases of metastatic tumors occurring in the jawbones, their clinical features , diagnostic workup and management. PATIENTS AND METHODS:The records of 8 patients with metastatic jaw lesions were reviewed. Demographic data, presenting symptoms, primary tumor site, r...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:
更新日期:2006-03-01 00:00:00
abstract::The use of medicines during pregnancy deserves special attention from dentists due to the potential risks to fetal development. The prescription of antimicrobial drugs during this period must be based not only on the etiology of the disease but also on the drug's effect on the embryo, which may be toxic, possibly lead...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章,评审
doi:
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate and compare the postoperative effect of a topic gel containing chlorhexidine, chitosan, allantoine and dexpanthenol versus a placebo for pain and inflammation control after third molar surgery. MATERIAL AND METHODS:A gel combining 0.2% chlorhexdine, 0.5% chitosan, 5% de...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章,随机对照试验
doi:10.4317/medoral.23661
更新日期:2020-09-01 00:00:00
abstract::Denture hyperplasia is a reactive lesion of the oral mucosa, usually associated to an ill-fitting denture. This lesion is easily diagnosed and in some cases distinct microscopic variations such as osseous, oncocytic and squamous metaplasia may be found. These metaplastic alterations probably are associated with the ly...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:
更新日期:2005-07-01 00:00:00
abstract:AIM:To compare the apical sealing in mesio-buccal canals of extracted molars obturated with low-temperature thermoplasticized gutta-percha or cold lateral condensation techniques using a .06 or a .02 mm/mm tapered gutta-percha master cone. The secondary aim was to evaluate the depth of spreader penetration in root cana...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2007-03-01 00:00:00
abstract::The periodontal disease is conformed by a group of illnesses affecting the gums and dental support structures. They are caused by certain bacteria found in the bacterial plaque. These bacteria are essential to the onset of illness; however, there are predisposing factors in both the host and the microorganisms that wi...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4321/s1699-65852005000300002
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Extensive literature exists about the use of the BFP in the treatment of oral defects but, to our knowledge, no article refers to the use of the BFP as a substitute of the membrane barriers for treatment of peri-implant bone defects. The aim was to evaluate the use of the buccal fat pad as a coating material...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.20212
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVE:The aim of the study was to evaluate the patient s perception of the events during and after an osteogenic alveolar distraction (OAD) procedure MATERIALS AND METHODS:A total of fifty-five (55) osteogenic alveolar distraction (OAD) procedures were performed in fifty (50) patients, who then were asked to answe...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:
更新日期:2007-05-01 00:00:00
abstract:OBJECTIVE:To compare the long-term sealing ability of GuttaFlow® using different obturation techniques. STUDY DESIGN:Three hundred teeth, prepared with a crown-down technique, were divided into thirty experimental groups (n=10) to evaluate the apical and coronal leakage, at 3, 30 and 120 days, of lateral compaction gu...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.15.e936
更新日期:2010-11-01 00:00:00
abstract::A series of 20 consecutive patients with an osteosarcoma of the jaws has been evaluated with regard to possible professional diagnostic delay. When set at an arbitrarily chosen period beyond three months, professional delay occurred in 15 patients, the mean being 21 months and the median 11 months. In five of the 15 p...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.24334
更新日期:2020-11-01 00:00:00
abstract:BACKGROUND:The study of osteoblasts and their osteogenic functions is essential in order to understand them and their applications in implantology. In this sense, this study try to study BMP-2 production and bone matrix deposition, in addition to other biological variables, in osteoblasts cultured on a rough double aci...
journal_title:Medicina oral, patologia oral y cirugia bucal
pub_type: 杂志文章
doi:10.4317/medoral.22071
更新日期:2017-09-01 00:00:00