Abstract:
:Genome stability of human embryonic stem cells (hESC) is an important issue because even minor genetic alterations can negatively impact cell functionality and safety. The incorrect repair of DNA double-stranded breaks (DSBs) is the ultimate cause of the formation of chromosomal aberrations. Using G2 radiosensitivity assay, we analyzed chromosomal aberrations in pluripotent stem cells and somatic cells. The chromatid exchange aberration rates in hESCs increased manifold 2 hours after irradiation as compared with their differentiated derivatives, but the frequency of radiation-induced chromatid breaks was similar. The rate of radiation-induced chromatid exchanges in hESCs and differentiated cells exhibited a quadratic dose response, revealing two-hit mechanism of exchange formation suggesting that a non-homologous end joining (NHEJ) repair may contribute to their formation. Inhibition of DNA-PK, a key NHEJ component, by NU7026 resulted in a significant decrease in radiation-induced chromatid exchanges in hESCs but not in somatic cells. In contrast, NU7026 treatment increased the frequency of radiation-induced breaks to a similar extent in pluripotent and somatic cells. Thus, DNA-PK dependent NHEJ efficiently participates in the elimination of radiation-induced chromatid breaks during the late G2 in both cell types and DNA-PK activity leads to a high level of misrejoining specifically in pluripotent cells.
journal_name
Aging (Albany NY)journal_title
Agingauthors
Bogomazova AN,Lagarkova MA,Tskhovrebova LV,Shutova MV,Kiselev SLdoi
10.18632/aging.100336subject
Has Abstractpub_date
2011-06-01 00:00:00pages
584-96issue
6issn
1945-4589pii
100336journal_volume
3pub_type
杂志文章相关文献
Aging-US文献大全abstract::Glomerulosclerosis and renal interstitial fibrosis occur with the aging kidney. In this study, we examined the expression of miR-21, peroxisome proliferator-activated receptor(PPARα), hypoxia-inducible factor(HIF-1α) in the kidney of 3-month-old rats fed ad libitum (YAL), 24-month-old rats fed ad libitum (OAL) and 24-...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103591
更新日期:2020-08-27 00:00:00
abstract::Chondroitin sulfate (CS) proteoglycans in perineuronal nets (PNNs) from the central nervous system (CNS) are involved in the control of plasticity and memory. Removing PNNs reactivates plasticity and restores memory in models of Alzheimer's disease and ageing. Their actions depend on the glycosaminoglycan (GAG) chains...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101256
更新日期:2017-06-28 00:00:00
abstract::Gradual changes in the DNA methylation landscape occur throughout aging virtually in all human tissues. A widespread reduction of 5-methylcytosine (5mC), associated with highly reproducible site-specific hypermethylation, characterizes the genome in aging. Therefore, an equilibrium seems to exist between general and d...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101022
更新日期:2016-08-29 00:00:00
abstract::Many studies have reported that estrogen (E2) promotes lung cancer by binding to nuclear estrogen receptors (ER), and altering ER related nuclear protein expressions. With the GEO database analysis, Human centromere protein F (CENPF) is highly expressed in lung adenocarcinoma (LUAD), and the co-expression of CENPF and...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.202303
更新日期:2021-01-10 00:00:00
abstract::The study was aimed at evaluation of the role of secondary oxidative stress in the stress-induced premature senescence (SIPS) of human fibroblasts induced by H2O2. Two fibroblast lines were used: lung MRC-5 and ear H8F2p25LM fibroblasts. The lines differed considerably in sensitivity to H2O2 (IC50 of 528 and 33.5 μM, ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102730
更新日期:2020-01-21 00:00:00
abstract::The gerosuppressant metformin operates as an efficient inhibitor of the mTOR/S6K1 gerogenic pathway due to its ability to ultimately activate the energy-sensor AMPK. If an aging-related decline in the AMPK sensitivity to cellular stress is a crucial event for mTOR-driven aging and aging-related diseases, including can...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100523
更新日期:2012-12-01 00:00:00
abstract::Metabolic syndrome, a network of medical disorders that greatly increase the risk for developing metabolic and cardiovascular diseases, has reached epidemic levels in many areas of today's world. Despite this alarming medicare situation, scientific understandings on the root mechanisms of metabolic syndrome are still ...
journal_title:Aging
pub_type: 杂志文章,评审
doi:10.18632/aging.100431
更新日期:2012-02-01 00:00:00
abstract::Oxidative stress and inflammation play vital roles in Parkinson's disease (PD) development. Thus, telomere length is expected to be shortened in this disease, but current data are inconclusive. We performed a case-control study of 261 patients with PD and 270 sex and age-matched healthy controls treated at the Peking ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103878
更新日期:2020-10-29 00:00:00
abstract::Alterations in KEAP1/ NF-E2 p45-related factor 2 (NFE2L2/Nrf2) signaling pathway have been reported in 23% lung adenocarcinoma patients, suggesting that deregulation of the pathway is a major cancer driver. Here we report that mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) drives tumor growth and drug re...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102531
更新日期:2019-12-06 00:00:00
abstract::Telomeres are short segments in chromosome ends, the length of which is reduced during cell lifecycles. We examined the association of mean leukocyte telomere length (LTL) and short telomere proportion (STP) with hemodynamic variables in normotensive and never-treated hypertensive volunteers (n=566, 19-72 years). STP ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103459
更新日期:2020-06-23 00:00:00
abstract::In this study, we constructed a model using a Cox proportional hazards model based on the expression of eight immune-related genes that were associated with prognosis in prostate cancer: EDNRB, ANGPTL2, TNFSF15, TNFRSF10D, EDN2, BMP2, NLRP14, and PLK1. We then identified associations between risk scores calculated wit...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103921
更新日期:2020-11-10 00:00:00
abstract::Background: Aging has often been linked to age-related vascular disorders. The elucidation of the putative genes and pathways underlying vascular aging likely provides useful insights into vascular diseases at advanced ages. Transcriptional regulatory network analysis is the key to describing genetic interactions betw...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102275
更新日期:2019-09-12 00:00:00
abstract:BACKGROUND:Gastric cancer is a common malignancy and had poor response to treatment due to its strong heterogeneity. This study aimed to identify essential genes associated with diffuse type gastric cancer and construct a powerful prognostic model. RESULTS:We conducted a weighted gene co-expression network analysis (W...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103743
更新日期:2020-09-11 00:00:00
abstract::Studies in young mammals on the molecular effects of food restriction leading to prolong adult life are scares. Here, we used high-throughput quantitative proteomic analysis of whole rat livers to address the molecular basis for growth arrest and the apparent life-prolonging phenotype of the food restriction regimen. ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101009
更新日期:2016-08-01 00:00:00
abstract::For nearly a decade, sorafenib has served as a first-line chemotherapeutic drug for the treatment of hepatocellular carcinoma (HCC), but it displays only limited efficacy against advanced drug-resistant HCC. Regorafenib, the first second-line drug approved for treatment after sorafenib failure, can reverse resistance ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104028
更新日期:2020-11-16 00:00:00
abstract::Histones and their variants are subjected to several post-translational modifications (PTMs). Histones PTMs play an important role in the regulation of gene expression and are critical for the development and progression of many types of cancer, including breast cancer. In this study, we used two-dimensional TAU/SDS e...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102577
更新日期:2019-12-08 00:00:00
abstract::The broccoli-derived isothiocyanate sulforaphane inhibits inflammation, oxidative stress and cancer, but its effect on healthspan and longevity are unclear. We used the C. elegans nematode model and fed the wildtype and 9 mutant strains ±sulforaphane. The lifespan, phenotype, pharyngeal pumping, mobility, lipofuscin a...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.202512
更新日期:2021-01-20 00:00:00
abstract::Hyperglycemia impairs insulin secretion as well as insulin action, being recognized as the glucotoxicity that accelerates diabetes. However, the mechanism underlying the glucotoxicity in pancreatic β-cells is not thoroughly understood. Hyperglycemia alters glucose metabolism within β-cells and interstitial conditions ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100647
更新日期:2014-03-01 00:00:00
abstract::We explored whether acute atorvastatin treatment would improve clinical outcomes and reduce the incidence of cerebral vasospasm after aneurysmal subarachnoid hemorrhage in elderly Chinese adults. Patients (60 to 90 years old) were admitted to intensive care units after surgery to clip or embolize their aneurysms. We a...
journal_title:Aging
pub_type: 杂志文章,随机对照试验
doi:10.18632/aging.102788
更新日期:2020-02-07 00:00:00
abstract::Programmed cell death 1 (PD-1) checkpoint inhibitor therapy leads to immune-related adverse events (irAEs). We sought to evaluate whether the development of irAEs correlates with the treatment response in Chinese patients with advanced melanoma. In this study, we conducted a retrospective study of advanced melanoma pa...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103285
更新日期:2020-06-08 00:00:00
abstract::Proliferating cell nuclear antigen binding factor (encoded by KIAA0101/PCLAF) regulates DNA synthesis and cell cycle progression; however, whether the level of KIAA0101 mRNA in lung adenocarcinoma is related to prognosis and tumor immune infiltration is unknown. In patients with lung adenocarcinoma, the differential e...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104144
更新日期:2020-11-20 00:00:00
abstract:OBJECTIVE:We analyzed clinical parameters and risk factors for coronavirus disease 2019 (COVID-19)-related liver damage. RESULTS:Of the 332 COVID-19 patients, 306 and 26 were included in the non-critical and critical groups, respectively. The median time from onset to admission was 4.0 (2.0-8.0) days. Of the 332 COVID...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103860
更新日期:2020-10-01 00:00:00
abstract:INTRODUCTION:Circulating non-coding RNA is an ideal source to discover novel biomarkers for non-invasive screening. However, studies for the discovery of universal miRNAs in serum and exosomes for breast cancer early diagnosis are limited. METHODS:Based on bioinformatic analysis, in vitro and in vivo studies were perf...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.202388
更新日期:2021-01-20 00:00:00
abstract::Adipocyte differentiation involves a series of highly synergistic processes, including clone amplification, proliferation arrest, and terminal differentiation. However, the mechanisms that control these different steps remain unclear. Emerging studies support that miRNAs play an important role in regulating adipogenes...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103649
更新日期:2020-09-14 00:00:00
abstract::Aging is associated with impaired angiogenesis and lung alveolar regeneration, which contributes to the increased susceptibility to chronic lung diseases (CLD). We have reported that the Wnt ligand co-receptor, low-density lipoprotein receptor-related protein 5 (LRP5), stimulates angiogenesis and lung alveolar regener...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101722
更新日期:2019-01-05 00:00:00
abstract::In this retrospective study we assessed the efficacy and safety of tocilizumab in patients with critical or severe coronavirus disease 2019 (COVID-19). We enrolled 181 patients admitted to Huoshenshan Hospital (Wuhan, China) with confirmed COVID-19 between January 2020 and February 2020. Ninety-two patients were treat...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103988
更新日期:2020-10-08 00:00:00
abstract::Poly(ADP-ribosyl) polymerases (PARPs) have traditionally been linked to chromosome maintenance and DNA repair. Recent findings identify PARPs as key modulators of metabolism through their influence on SIRT1 activity, hinting to a possible role of PARPs as longevity regulators. ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100326
更新日期:2011-05-01 00:00:00
abstract::To investigate the relation of higher apolipoprotein B/apolipoprotein AI (apoB/AI) ratio with the risk of suffering intracranial atherosclerotic stenosis (ICAS) in both stroke and non-stroke population, we enrolled 1138 patients with acute ischemic stroke (359 with ICAS, 779 without ICAS) and 1072 non-stroke controls ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102216
更新日期:2019-09-03 00:00:00
abstract::TET family members (TETs) encode proteins that represent crucial factors in the active DNA demethylation pathway. Evidence has proved that TET2 mutation is associated with leukemogenesis, drug response, and prognosis in acute myeloid leukemia (AML). However, few studies revealed the TETs expression and its clinical si...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102928
更新日期:2020-03-25 00:00:00
abstract::This study aimed to investigate the risk factors of second primary cancer among female breast cancer (BC) survivors, with emphasis on the prediction of the individual risk conditioned on the patient's characteristics. We identified 208,474 BC patients diagnosed between 2004 and 2010 from the Surveillance, Epidemiology...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103939
更新日期:2020-10-13 00:00:00