The use of a mobile laboratory unit in support of patient management and epidemiological surveillance during the 2005 Marburg Outbreak in Angola.


BACKGROUND:Marburg virus (MARV), a zoonotic pathogen causing severe hemorrhagic fever in man, has emerged in Angola resulting in the largest outbreak of Marburg hemorrhagic fever (MHF) with the highest case fatality rate to date. METHODOLOGY/PRINCIPAL FINDINGS:A mobile laboratory unit (MLU) was deployed as part of the World Health Organization outbreak response. Utilizing quantitative real-time PCR assays, this laboratory provided specific MARV diagnostics in Uige, the epicentre of the outbreak. The MLU operated over a period of 88 days and tested 620 specimens from 388 individuals. Specimens included mainly oral swabs and EDTA blood. Following establishing on site, the MLU operation allowed a diagnostic response in <4 hours from sample receiving. Most cases were found among females in the child-bearing age and in children less than five years of age. The outbreak had a high number of paediatric cases and breastfeeding may have been a factor in MARV transmission as indicated by the epidemiology and MARV positive breast milk specimens. Oral swabs were a useful alternative specimen source to whole blood/serum allowing testing of patients in circumstances of resistance to invasive procedures but limited diagnostic testing to molecular approaches. There was a high concordance in test results between the MLU and the reference laboratory in Luanda operated by the US Centers for Disease Control and Prevention. CONCLUSIONS/SIGNIFICANCE:The MLU was an important outbreak response asset providing support in patient management and epidemiological surveillance. Field laboratory capacity should be expanded and made an essential part of any future outbreak investigation.


PLoS Negl Trop Dis


Grolla A,Jones SM,Fernando L,Strong JE,Ströher U,Möller P,Paweska JT,Burt F,Pablo Palma P,Sprecher A,Formenty P,Roth C,Feldmann H




Has Abstract


2011-05-01 00:00:00














  • Spatial distribution of Taenia solium porcine cysticercosis within a rural area of Mexico.

    abstract::Cysticercosis is caused by Taenia solium, a parasitic disease that affects humans and rurally bred pigs in developing countries. The cysticercus may localize in the central nervous system of the human, causing neurocysticercosis, the most severe and frequent form of the disease. There appears to be an association betw...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Morales J,Martínez JJ,Rosetti M,Fleury A,Maza V,Hernandez M,Villalobos N,Fragoso G,de Aluja AS,Larralde C,Sciutto E

    更新日期:2008-01-01 00:00:00

  • Correction: The Influence of Socio-economic, Behavioural and Environmental Factors on Taenia spp. Transmission in Western Kenya: Evidence from a Cross-Sectional Survey in Humans and Pigs.

    abstract::[This corrects the article DOI: 10.1371/journal.pntd.0004223.]. ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 已发布勘误


    authors: Wardrop NA,Thomas LF,Atkinson PM,de Glanville WA,Cook EA,Wamae CN,Gabriël S,Dorny P,Harrison LJ,Fèvre EM

    更新日期:2016-01-13 00:00:00

  • Genetic diversity and evolution of Hantaan virus in China and its neighbors.

    abstract:BACKGROUND:Hantaan virus (HTNV; family Hantaviridae, order Bunyavirales) causes hemorrhagic fever with renal syndrome (HFRS), which has raised serious concerns in Eurasia, especially in China, Russia, and South Korea. Previous studies reported genetic diversity and phylogenetic features of HTNV in different parts of Ch...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Li N,Li A,Liu Y,Wu W,Li C,Yu D,Zhu Y,Li J,Li D,Wang S,Liang M

    更新日期:2020-08-20 00:00:00

  • TLR9 activation dampens the early inflammatory response to Paracoccidioides brasiliensis, impacting host survival.

    abstract:BACKGROUND:Paracoccidioides brasiliensis causes paracoccidioidomycosis, one of the most prevalent systemic mycosis in Latin America. Thus, understanding the characteristics of the protective immune response to P. brasiliensis is of interest, as it may reveal targets for disease control. The initiation of the immune res...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Menino JF,Saraiva M,Gomes-Alves AG,Lobo-Silva D,Sturme M,Gomes-Rezende J,Saraiva AL,Goldman GH,Cunha C,Carvalho A,Romani L,Pedrosa J,Castro AG,Rodrigues F

    更新日期:2013-07-25 00:00:00

  • QTL mapping of genome regions controlling temephos resistance in larvae of the mosquito Aedes aegypti.

    abstract:INTRODUCTION:The mosquito Aedes aegypti is the principal vector of dengue and yellow fever flaviviruses. Temephos is an organophosphate insecticide used globally to suppress Ae. aegypti larval populations but resistance has evolved in many locations. METHODOLOGY/PRINCIPAL FINDINGS:Quantitative Trait Loci (QTL) control...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Reyes-Solis Gdel C,Saavedra-Rodriguez K,Suarez AF,Black WC 4th

    更新日期:2014-10-16 00:00:00

  • Antimony to Cure Visceral Leishmaniasis Unresponsive to Liposomal Amphotericin B.

    abstract::We report on 4 patients (1 immunocompetent, 3 immunosuppressed) in whom visceral leishmaniasis had become unresponsive to (or had relapsed after) treatment with appropriate doses of liposomal amphotericin B. Under close follow-up, full courses of pentavalent antimony were administered without life-threatening adverse ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Morizot G,Jouffroy R,Faye A,Chabert P,Belhouari K,Calin R,Charlier C,Miailhes P,Siriez JY,Mouri O,Yera H,Gilquin J,Tubiana R,Lanternier F,Mamzer MF,Legendre C,Peyramond D,Caumes E,Lortholary O,Buffet P

    更新日期:2016-01-06 00:00:00

  • Modelling targeted rabies vaccination strategies for a domestic dog population with heterogeneous roaming patterns.

    abstract::Australia is currently canine rabies free. However, communities located on the northern coastline-such as the Northern Peninsula Area (NPA), Queensland-are at risk of an incursion due to their large populations of susceptible free-roaming dogs and proximity to rabies-infected Indonesian islands. A rabies-spread model ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Hudson EG,Brookes VJ,Dürr S,Ward MP

    更新日期:2019-07-08 00:00:00

  • Zika virus: An updated review of competent or naturally infected mosquitoes.

    abstract::Zika virus (ZIKV) is an arthropod-borne virus (arbovirus) that recently caused outbreaks in the Americas. Over the past 60 years, this virus has been observed circulating among African, Asian, and Pacific Island populations, but little attention has been paid by the scientific community until the discovery that large-...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Epelboin Y,Talaga S,Epelboin L,Dusfour I

    更新日期:2017-11-16 00:00:00

  • Cathepsin F cysteine protease of the human liver fluke, Opisthorchis viverrini.

    abstract:BACKGROUND:The liver fluke Opisthorchis viverrini is classified as a class I carcinogen due to the association between cholangiocarcinoma and chronic O. viverrini infection. During its feeding activity within the bile duct, the parasite secretes several cathepsin F cysteine proteases that may induce or contribute to th...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Pinlaor P,Kaewpitoon N,Laha T,Sripa B,Kaewkes S,Morales ME,Mann VH,Parriott SK,Suttiprapa S,Robinson MW,To J,Dalton JP,Loukas A,Brindley PJ

    更新日期:2009-01-01 00:00:00

  • The Role of Nurses and Community Health Workers in Confronting Neglected Tropical Diseases in Sub-Saharan Africa: A Systematic Review.

    abstract:INTRODUCTION:Neglected tropical diseases produce an enormous burden on many of the poorest and most disenfranchised populations in sub-Saharan Africa. Similar to other developing areas throughout the world, this region's dearth of skilled health providers renders Western-style primary care efforts to address such disea...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Corley AG,Thornton CP,Glass NE

    更新日期:2016-09-15 00:00:00

  • Both living bacteria and eukaryotes in the mosquito gut promote growth of larvae.

    abstract::We recently reported that larval stage Aedes aegypti and several other species of mosquitoes grow when living bacteria are present in the gut but do not grow when living bacteria are absent. We further reported that living bacteria induce a hypoxia signal in the gut, which activates hypoxia-induced transcription facto...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Valzania L,Martinson VG,Harrison RE,Boyd BM,Coon KL,Brown MR,Strand MR

    更新日期:2018-07-06 00:00:00

  • Quantifying the burden of rhodesiense sleeping sickness in Urambo District, Tanzania.

    abstract:BACKGROUND:Human African trypanosomiasis is a severely neglected vector-borne disease that is always fatal if untreated. In Tanzania it is highly focalised and of major socio-economic and public health importance in affected communities. OBJECTIVES:This study aimed to estimate the public health burden of rhodesiense H...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Matemba LE,Fèvre EM,Kibona SN,Picozzi K,Cleaveland S,Shaw AP,Welburn SC

    更新日期:2010-11-02 00:00:00

  • Quantifying sociodemographic heterogeneities in the distribution of Aedes aegypti among California households.

    abstract::The spread of Aedes aegypti in California and other regions of the U.S. has increased the need to understand the potential for local chains of Ae. aegypti-borne virus transmission, particularly in arid regions where the ecology of these mosquitoes is less understood. For public health and vector control programs, it i...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Donnelly MAP,Kluh S,Snyder RE,Barker CM

    更新日期:2020-07-21 00:00:00

  • Heterogeneities in Leishmania infantum infection: using skin parasite burdens to identify highly infectious dogs.

    abstract:BACKGROUND:The relationships between heterogeneities in host infection and infectiousness (transmission to arthropod vectors) can provide important insights for disease management. Here, we quantify heterogeneities in Leishmania infantum parasite numbers in reservoir and non-reservoir host populations, and relate this ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Courtenay O,Carson C,Calvo-Bado L,Garcez LM,Quinnell RJ

    更新日期:2014-01-09 00:00:00

  • Interaction of ATP with a small heat shock protein from Mycobacterium leprae: effect on its structure and function.

    abstract::Adenosine-5'-triphosphate (ATP) is an important phosphate metabolite abundantly found in Mycobacterium leprae bacilli. This pathogen does not derive ATP from its host but has its own mechanism for the generation of ATP. Interestingly, this molecule as well as several antigenic proteins act as bio-markers for the detec...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Nandi SK,Chakraborty A,Panda AK,Ray SS,Kar RK,Bhunia A,Biswas A

    更新日期:2015-03-26 00:00:00

  • In vitro studies of Rickettsia-host cell interactions: Confocal laser scanning microscopy of Rickettsia helvetica-infected eukaryotic cell lines.

    abstract::Rickettsia (R.) helvetica is the most prevalent rickettsia found in Ixodes ricinus ticks in Germany. Several studies reported antibodies against R. helvetica up to 12.5% in humans investigated, however, fulminant clinical cases are rare indicating a rather low pathogenicity compared to other rickettsiae. We investigat...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Speck S,Kern T,Aistleitner K,Dilcher M,Dobler G,Essbauer S

    更新日期:2018-02-12 00:00:00

  • Gene flow, subspecies composition, and dengue virus-2 susceptibility among Aedes aegypti collections in Senegal.

    abstract:BACKGROUND:Aedes aegypti, the "yellow fever mosquito", is the primary vector to humans of the four serotypes of dengue viruses (DENV1-4) and yellow fever virus (YFV) and is a known vector of Chikungunya virus. There are two recognized subspecies of Ae. aegypti sensu latu (s.l.): the presumed ancestral form, Ae. aegypti...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Sylla M,Bosio C,Urdaneta-Marquez L,Ndiaye M,Black WC 4th

    更新日期:2009-01-01 00:00:00

  • Emerging trends of Zika apprehension in an epidemic setting.

    abstract:BACKGROUND:French Guiana is a territory that has a decades-long history of dengue outbreaks and more recently, in 2014, a chikungunya outbreak. Zika virus (ZIKV) emerged in late 2015 and subsequently led to an important outbreak. METHODOLOGY/PRINCIPAL FINDINGS:A cross-sectional phone survey was conducted among the gen...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Fritzell C,Raude J,Kazanji M,Flamand C

    更新日期:2018-01-25 00:00:00

  • Impairment of T cell function in parasitic infections.

    abstract::In mammals subverted as hosts by protozoan parasites, the latter and/or the agonists they release are detected and processed by sensors displayed by many distinct immune cell lineages, in a tissue(s)-dependent context. Focusing on the T lymphocyte lineage, we review our present understanding on its transient or durabl...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Rodrigues V,Cordeiro-da-Silva A,Laforge M,Ouaissi A,Akharid K,Silvestre R,Estaquier J

    更新日期:2014-02-13 00:00:00

  • Rodent-borne diseases and their public health importance in Iran.

    abstract:BACKGROUND:Rodents are reservoirs and hosts for several zoonotic diseases such as plague, leptospirosis, and leishmaniasis. Rapid development of industry and agriculture, as well as climate change throughout the globe, has led to change or increase in occurrence of rodent-borne diseases. Considering the distribution of...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Rabiee MH,Mahmoudi A,Siahsarvie R,Kryštufek B,Mostafavi E

    更新日期:2018-04-19 00:00:00

  • Understanding the persistence of plague foci in Madagascar.

    abstract::Plague, a zoonosis caused by Yersinia pestis, is still found in Africa, Asia, and the Americas. Madagascar reports almost one third of the cases worldwide. Y. pestis can be encountered in three very different types of foci: urban, rural, and sylvatic. Flea vector and wild rodent host population dynamics are tightly co...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Andrianaivoarimanana V,Kreppel K,Elissa N,Duplantier JM,Carniel E,Rajerison M,Jambou R

    更新日期:2013-11-07 00:00:00

  • Patients infected with Mycobacterium africanum versus Mycobacterium tuberculosis possess distinct intestinal microbiota.

    abstract:BACKGROUND:Mycobacterium tuberculosis complex (MTBC), the causative agent of tuberculosis (TB), is composed of eight subspecies. TB in West Africa, in contrast to other geographical regions, is caused by Mycobacterium africanum (MAF) in addition to M. tuberculosis (MTB), with both infections presenting similar symptoms...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Namasivayam S,Diarra B,Diabate S,Sarro YDS,Kone A,Kone B,Tolofoudie M,Baya B,Diakite MT,Kodio O,Cohen K,Holl J,Achenbach CJ,Chatterjee S,Murphy RL,Bishai W,Diallo S,Sher A,Maiga M

    更新日期:2020-05-13 00:00:00

  • Assessment of control strategies against Clonorchis sinensis infection based on a multi-group dynamic transmission model.

    abstract::Clonorchiasis is one of the most important food-borne trematodiases affecting millions of people. Strategies were recommended by different organizations and control programmes were implemented but mostly in short-time periods. It's important to assess the long-term benefits and sustainability of possible control strat...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Huang XH,Qian MB,Zhu GH,Fang YY,Hao YT,Lai YS

    更新日期:2020-03-27 00:00:00

  • Animal reservoir, natural and socioeconomic variations and the transmission of hemorrhagic fever with renal syndrome in Chenzhou, China, 2006-2010.

    abstract:BACKGROUND:China has the highest incidence of hemorrhagic fever with renal syndrome (HFRS) worldwide. Reported cases account for 90% of the total number of global cases. By 2010, approximately 1.4 million HFRS cases had been reported in China. This study aimed to explore the effect of the rodent reservoir, and natural ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Xiao H,Tian HY,Gao LD,Liu HN,Duan LS,Basta N,Cazelles B,Li XJ,Lin XL,Wu HW,Chen BY,Yang HS,Xu B,Grenfell B

    更新日期:2014-01-09 00:00:00

  • Early Indicators of Fatal Leptospirosis during the 2010 Epidemic in Puerto Rico.

    abstract:BACKGROUND:Leptospirosis is a potentially fatal bacterial zoonosis that is endemic throughout the tropics and may be misdiagnosed as dengue. Delayed hospital admission of leptospirosis patients is associated with increased mortality. METHODOLOGY/PRINCIPAL FINDINGS:During a concurrent dengue/leptospirosis epidemic in P...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Sharp TM,Rivera García B,Pérez-Padilla J,Galloway RL,Guerra M,Ryff KR,Haberling D,Ramakrishnan S,Shadomy S,Blau D,Tomashek KM,Bower WA

    更新日期:2016-02-25 00:00:00

  • Prevalence and intensity of soil-transmitted helminthiasis, prevalence of malaria and nutritional status of school going children in honduras.

    abstract:BACKGROUND:Many small studies have been done in Honduras estimating soil-transmitted helminthiasis (STH) prevalence but a country-wide study was last done in 2005. The country has the highest burden of malaria among all Central American countries. The present study was done to estimate country-wide STH prevalence and i...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Mejia Torres RE,Franco Garcia DN,Fontecha Sandoval GA,Hernandez Santana A,Singh P,Mancero Bucheli ST,Saboya M,Paz MY

    更新日期:2014-10-16 00:00:00

  • Isolation of Infective Zika Virus from Urine and Saliva of Patients in Brazil.

    abstract:BACKGROUND:Zika virus (ZIKV) is an emergent threat provoking a worldwide explosive outbreak. Since January 2015, 41 countries reported autochthonous cases. In Brazil, an increase in Guillain-Barré syndrome and microcephaly cases was linked to ZIKV infections. A recent report describing low experimental transmission eff...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Bonaldo MC,Ribeiro IP,Lima NS,Dos Santos AA,Menezes LS,da Cruz SO,de Mello IS,Furtado ND,de Moura EE,Damasceno L,da Silva KA,de Castro MG,Gerber AL,de Almeida LG,Lourenço-de-Oliveira R,Vasconcelos AT,Brasil P

    更新日期:2016-06-24 00:00:00

  • In Vitro Study of Taenia solium Postoncospheral Form.

    abstract:BACKGROUND:The transitional period between the oncosphere and the cysticercus of Taenia solium is the postoncospheral (PO) form, which has not yet been completely characterized. The aim of this work was to standardize a method to obtain T. solium PO forms by in vitro cultivation. We studied the morphology of the PO for...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Chile N,Clark T,Arana Y,Ortega YR,Palma S,Mejia A,Angulo N,Kosek JC,Kosek M,Gomez-Puerta LA,Garcia HH,Gavidia CM,Gilman RH,Verastegui M,Cysticercosis Working Group in Peru.

    更新日期:2016-02-10 00:00:00

  • The iron distribution and magnetic properties of schistosome eggshells: implications for improved diagnostics.

    abstract:BACKGROUND:Schistosoma mansoni and Schistosoma japonicum are the most frequent causative agents of human intestinal schistosomiasis. Approximately 200 million people in the world are infected with schistosomes. Diagnosis of schistosomiasis is often difficult. High percentages of low level infections are missed in routi...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Karl S,Gutiérrez L,Lucyk-Maurer R,Kerr R,Candido RR,Toh SQ,Saunders M,Shaw JA,Suvorova A,Hofmann A,House MJ,Woodward RC,Graeff-Teixera C,St Pierre TG,Jones MK

    更新日期:2013-05-16 00:00:00

  • Spatio-temporal Genetic Structuring of Leishmania major in Tunisia by Microsatellite Analysis.

    abstract::In Tunisia, cases of zoonotic cutaneous leishmaniasis caused by Leishmania major are increasing and spreading from the south-west to new areas in the center. To improve the current knowledge on L. major evolution and population dynamics, we performed multi-locus microsatellite typing of human isolates from Tunisian go...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Harrabi M,Bettaieb J,Ghawar W,Toumi A,Zaâtour A,Yazidi R,Chaâbane S,Chalghaf B,Hide M,Bañuls AL,Ben Salah A

    更新日期:2015-08-24 00:00:00