Abstract：
:Microbial communities are key components of all ecosystems, but characterization of their complete genomic structure remains challenging. Typical analysis tends to elude the complexity of the mixes in terms of species, strains, as well as extrachromosomal DNA molecules. Recently, approaches have been developed that bins DNA contigs into individual genomes and episomes according to their 3D contact frequencies. Those contacts are quantified by chromosome conformation capture experiments (3C, Hi-C), also known as proximity-ligation approaches, applied to metagenomics samples. Here, we present a simple computational pipeline that allows to recover high-quality Metagenomics Assemble Genomes (MAGs) starting from metagenomic 3C or Hi-C datasets and a metagenome assembly.
journal_name
Methods Mol Bioljournal_title
Methods in molecular biology (Clifton, N.J.)authors
Marbouty M,Koszul Rdoi
10.1007/978-1-0716-1390-0_8keywords:
["Binning","Chromosome conformation capture","Hi-C, metaHiC","Meta3C","Metagenomes assemble genomes","Metagenomic Hi-C"]subject
Has Abstractpub_date
2022-01-01 00:00:00pages
163-181eissn
1064-3745issn
1940-6029journal_volume
2301pub_type
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