Abstract：
BACKGROUND:We set out to detect whether morphine is present in tissue taken from a patient with intractable temporal lobe epilepsy and to characterize the presence and nature of mu opiate receptor subtypes in this tissue. CASE REPORT:In temporal lobe tissue, resected during anteromedial temporal lobectomy for intractable focal epilepsy, morphine was identified by quantitative radioimmunoassay (RIA) coupled to electrochemical detection via high-pressure liquid chromatography (HPLC). In addition, RNA isolated from the medial and lateral temporal lobe specimens was analyzed by conventional and real time reverse transcriptase-polymerase chain reaction (RT-PCR) for the expression of different human receptor gene transcripts. RIA revealed the presence of morphine at 3.4 nanograms per gram of tissue wet weight. Using RT-PCR and a primer specifically set for the mu3 (550 base pair fragment) and mu4 (880 base pair fragment) MOR splice variants, a mu4 splice variant was identified in both brain sections. CONCLUSIONS:This human brain tissue study of a subject with temporal lobe epilepsy documents the presence of endogenous morphine and of a mu4 splice variant. These findings may have implications for our understanding of the mechanism of temporal lobe epilepsy.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Fricchione G,Zhu W,Cadet P,Mantione KJ,Bromfield E,Madsen J,DeGirolami U,Dworetzky B,Vaccaro B,Black P,Stefano GBsubject
Has Abstractpub_date
2008-06-01 00:00:00pages
CS45-49issue
6eissn
1234-1010issn
1643-3750pii
859023journal_volume
14pub_type
杂志文章abstract：BACKGROUND:Vasospasm is a major cause of ischemic neurological deficits developing after subarachnoid hemorrhage. The goal was to identify hemodynamic changes and the presence of clinical vasospasm in patients suffering from subarachnoid hemorrhage secondary to ruptured intracranial aneurysms. MATERIAL/METHODS:Pre- an...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2007-10-01 00:00:00
abstract：:BACKGROUND Transplantation with allogeneic cells has become a promising modality for cancer therapy, which can induce graft-versus-tumor (GVT) effect. This study was aimed at assessing the safety, efficacy, and tissue type GVT (tGVT) response of transplantation with allogeneic skin tumors to treat chemically-induced s...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.900148
更新日期：2016-09-02 00:00:00
abstract：BACKGROUND:Cajal bodies (CB) are distinct sub-nuclear domains rich in small nuclear ribonucleoprotein particles (snRNPs); they are involved in pre-mRNA processing. Lupus erythematosus (SLE) is an autoimmune disease characterized by autoantibody production against different nuclear molecules, including those involved in...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：:The Fig 4C and 4D are the same, but the author showed them as two parallel groups. Reference: Cardioprotective Effect of Isosorbide Dinitrate Postconditioning Against Rat Myocardial Ischemia-Reperfusion Injury In Vivo Xinwen Zhao, Mengjuan Wang, Mei Li, Na Wu, Dalin Song Med Sci Monit 2019;25: 1629-1636 10.12659/MSM.9...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,撤回出版物
doi：10.12659/MSM.919342
更新日期：2019-08-26 00:00:00
abstract：BACKGROUND:The aim was to evaluate the dietary habits and some lifestyle characteristics of Greek adolescents 12-17 years of age in relation to the prevalence of overweight/obesity. MATERIAL/METHODS:During 2004-2005, 2008 school-aged adolescents (1021 male and 987 female) were selected from all public schools located ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2007-10-01 00:00:00
abstract：:BACKGROUND In this study we investigated changes in the status of antibiotic use in Tianjin since the implementation of the Antibiotic Stewardship Program (ASP) (2011-2013), as well as existing problems, strategies, and outcomes to promote rational clinical antibiotic use. MATERIAL AND METHODS A quasi-experimental stu...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.899848
更新日期：2017-02-09 00:00:00
abstract：BACKGROUND:Researchers are not in complete agreement over the extent to which specific language functions are subserved by certain brain areas. The purpose of this article was to determine neuroanatomical correlates of aphasia following cerebrovascular accident. MATERIAL/METHODS:The participants included 50 stroke pat...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：BACKGROUND:An increase in the prevalence of thyroid disease has been found with increasing iodine intake, since universal salt iodization (USI) was instituted throughout China in 1996. The aim of this study was to analyze the incidence of thyroid carcinoma (TC) before and after USI in Shenyang, a city of northeastern C...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.883736
更新日期：2013-01-14 00:00:00
abstract：:BACKGROUND Long non-coding RNAs (LncRNAs) could potentially function as diagnostic markers for gastric carcinoma. Nevertheless, the expression profile and biological feature of LncRNAs in early gastric cancer (EGC) remains to be explored. MATERIAL AND METHODS LncRNA expression microarray analysis was performed on 6 pa...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.917935
更新日期：2019-12-29 00:00:00
abstract：BACKGROUND:Tumor growth in multiple myeloma (MM) is regulated by the cytokine networks which are produced by myeloma cells and the microenvironment of the bone marrow. Interleukin-17 (IL-17) is implicated in the increased angiogenesis in the bone marrow of MM. Recent studies reported elevated levels of interleukin 17A ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.882204
更新日期：2012-01-01 00:00:00
abstract：:In December 2019, an outbreak of coronavirus infection emerged in Wuhan, Hubei Province of China, which is now named Coronavirus Disease 2019 (COVID-19). The outbreak spread rapidly within mainland China and globally. This paper reviews the different imaging modalities used in the diagnosis and treatment process of CO...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.924582
更新日期：2020-07-12 00:00:00
abstract：BACKGROUND:We aimed to analyze the risk factors and prognosis of acute kidney injury (AKI) after aortic arch repair in type A aortic dissection. MATERIAL AND METHODS:We included 155 patients undergoing arch repair surgery for type A aortic dissection from January 2009 to January 2014 in our hospital. Ninety-three pati...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.892492
更新日期：2015-03-04 00:00:00
abstract：:BACKGROUND Few studies have examined functional brain changes specifically associated with chemotherapy (CTx) in patients with lung cancer. This prospective longitudinal research aimed to explore the change in intrinsic brain activity by investigating patients with lung cancer after CTx. MATERIAL AND METHODS Sixteen p...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.921700
更新日期：2020-02-18 00:00:00
abstract：:BACKGROUND Cutaneous squamous cell carcinoma (cSCC) is the second most common type of non-melanoma skin cancer (NMSC) globally. The aims of this study were to further systematically clarify the potential association of rs833061 (-460 C>T) and rs1570360 (-1154 G>A), two SNPs of VEGF, with the risk of cSCC and the progn...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.896710
更新日期：2016-10-12 00:00:00
abstract：:BACKGROUND This study aimed to investigate the effects of metastasis-associated 1 (MTA1) gene expression and gene silencing in human non-small cell lung cancer (NSCLC) cells in vitro and on angiogenesis in tumor xenografts in vivo in nude mice. MATERIAL AND METHODS Human H460 and H1299 NSCLC cell lines underwent trans...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.912321
更新日期：2019-01-17 00:00:00
abstract：:BACKGROUND To investigate the effect of the BMP/Smad signaling pathway on fracture healing and osteogenic ability in senile osteoporotic fracture on humans and rats. MATERIAL AND METHODS Sixty-two patients and well-matched normal controls were enrolled for clinical observation. A rat model of senile osteoporotic fract...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.905958
更新日期：2018-06-25 00:00:00
abstract：BACKGROUND:Diaphragm palsy is common in the advanced stages of motor neuron disease and is a primary cause of fatal outcome However, respiratory failure is a presenting symptom of motor neuron disease in only a small number of patients. CASE REPORT:We present the case of a patient with dyspnea and orthopnea followed b...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：BACKGROUND:The aim of this study was to examine longitudinally the acquisition of reading fluency in a shallow orthography from the very beginning using eye movement recordings. The development of reading fluency is easier to examine in shallow (such as German, Finnish, or Italian) rather than in opaque (such as Englis...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
abstract：BACKGROUND:Chronic obstructive pulmonary disease (COPD) is an inflammatory disease associated with reactive oxygen species (ROS) production. The aim of this study was to evaluate the effect of Hypoxen treatment and the effect of HyFnC₆₀ on ROS production in patients' blood. MATERIAL/METHODS:ROS production in blood was...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 临床试验,杂志文章
doi：10.12659/msm.882460
更新日期：2012-02-01 00:00:00
abstract：BACKGROUND:Tuberculosis (TB) is a chronic bacterial infection caused by M. Tuberculosis. Studies of antibody response in TB have focussed mainly on their usefulness as a diagnostic serological tool, with little attention given to analysis of antibodies at the isotype and subclass level in relation to disease pathogenes...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：BACKGROUND:Detection of Leishmania parasite in patients by classical methods at the early stage of infection is limited. Several studies have shown that polymerase chain reaction (PCR) is more effective for the diagnosis of visceral Leishmaniasis (VL) in clinical samples. The purpose of this study was to develop a simp...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:It is suggested that perinatal supplementation of long-chain polyunsaturated fatty acids (LCPUFAs) especially; eicosapentaenoic and docosahexaenoic acids prevent schizophrenia in the adult. I propose that schizophrenia could be a low-grade systemic inflammatory disease with its origins in the perinatal period, probabl...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2004-12-01 00:00:00
abstract：BACKGROUND:Benzodiazepine binding sites distinct from the GABA-receptor-chloride-complex in the central nervous system have been recognized in many peripheral tissues, but their physiological role remains unexplained. Our study was undertaken to examine the effects of diazepam, clonazepam, and PK 11195, a peripheral be...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2002-04-01 00:00:00
abstract：:Currently it is the hope of both patients and investigators that human progenitor cells and stem cells can be widely used to replace dysfunctional cells within a tissue. It is speculated that such cells may prove to have the potential to treat or cure a myriad of diseases, including Parkinson's and Alzheimer's disease...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2002-11-01 00:00:00
abstract：BACKGROUND:The prevalence of pressure ulcers (PUs) ranges from 1 to 18% of in-patients and from 3 to 28% in long-term settings. The aim of our study was to verify how comorbidity and frailty influenced the course of PUs in a population of elderly subjects hospitalized in a long-term care setting. MATERIAL/METHODS:The ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2005-07-01 00:00:00
abstract：BACKGROUND:The prevalence of the deficiency of enzyme Lactase/phlorizin hydrolase (LPH) varies widely between different countries, with a low of 1-3% in Scandinavia and close to 100% in most of South-East Asia. Various carbohydrates are capable of enhancing LPH mRNA levels in the small intestine, and that transcription...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：BACKGROUND:The humanized anti-HER2 monoclonal antibody trastuzumab (Herceptin) is useful in the treatment of ErbB2-overexpressing breast cancers, but its efficiency is limited because development of resistance is common. In order to study the possibility of improving the efficacy of therapies directed against HER2/erbB...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2002-12-01 00:00:00
abstract：BACKGROUND:Mitigating urethral injury remains a great challenge for urologists due to lack of ideal biomaterials for urethroplasty. The application of amniotic membranes (AM) over other synthetic materials make it a better potential source for urethral reconstruction. We separated the basement layer of AM to obtain den...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.891042
更新日期：2014-11-26 00:00:00
abstract：:The endothelium, a continuous cellular monolayer lining the blood vessels, has an enormous range of important homeostatic roles. It serves and participates in highly active metabolic and regulatory functions including control of primary hemostasis, blood coagulation and fibrinolysis, platelet and leukocyte interaction...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2001-09-01 00:00:00
abstract：:BACKGROUND Alzheimer disease (AD) is a significant health issue for the elderly, and there are at present no clinically effective anti-AD agents. Prevention of Aß-induced neurotoxicity is proposed as a possible modality for treatment of AD. miR-33 has been proven to promote Aß secretion and impair Aß clearance in neur...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.921026
更新日期：2020-03-02 00:00:00
