当前位置： SCI文献检索 > Retrovirology期刊下所有文献 > DBR1 siRNA inhibition of HIV-1 replication.

DBR1 siRNA inhibition of HIV-1 replication.

Abstract：

BACKGROUND:HIV-1 and all retroviruses are related to retroelements of simpler organisms such as the yeast Ty elements. Recent work has suggested that the yeast retroelement Ty1 replicates via an unexpected RNA lariat intermediate in cDNA synthesis. The putative genomic RNA lariat intermediate is formed by a 2'-5' phosphodiester bond, like that found in pre-mRNA intron lariats and it facilitates the minus-strand template switch during cDNA synthesis. We hypothesized that HIV-1 might also form a genomic RNA lariat and therefore that siRNA-mediated inhibition of expression of the human RNA lariat de-branching enzyme (DBR1) expression would specifically inhibit HIV-1 replication. RESULTS:We designed three short interfering RNA (siRNA) molecules targeting DBR1, which were capable of reducing DBR1 mRNA expression by 80% and did not significantly affect cell viability. We assessed HIV-1 replication in the presence of DBR1 siRNA and found that DBR1 knockdown led to decreases in viral cDNA and protein production. These effects could be reversed by cotransfection of a DBR1 cDNA indicating that the inhibition of HIV-1 replication was a specific effect of DBR1 underexpression. CONCLUSION:These data suggest that DBR1 function may be needed to debranch a putative HIV-1 genomic RNA lariat prior to completion of reverse transcription.

journal_name

Retrovirology

journal_title

Retrovirology

authors

Ye Y,De Leon J,Yokoyama N,Naidu Y,Camerini D

doi

10.1186/1742-4690-2-63

subject

Has Abstract

pub_date

2005-10-18 00:00:00

pages

63

issn

1742-4690

pii

1742-4690-2-63

journal_volume

2

pub_type

杂志文章

相关文献

Retrovirology文献大全
  • Passive immunization of macaques with polyclonal anti-SHIV IgG against a heterologous tier 2 SHIV: outcome depends on IgG dose.

    abstract：BACKGROUND:A key goal for HIV-1 envelope immunogen design is the induction of cross-reactive neutralizing antibodies (nAbs). As AIDS vaccine recipients will not be exposed to strains exactly matching any immunogens due to multiple HIV-1 quasispecies circulating in the human population worldwide, heterologous SHIV chall...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-11-8

    authors： Sholukh AM,Byrareddy SN,Shanmuganathan V,Hemashettar G,Lakhashe SK,Rasmussen RA,Watkins JD,Vyas HK,Thorat S,Brandstoetter T,Mukhtar MM,Yoon JK,Novembre FJ,Villinger F,Landucci G,Forthal DN,Ratcliffe S,Tuero I,Robert-G

    更新日期：2014-01-20 00:00:00

  • NHEJ pathway is involved in post-integrational DNA repair due to Ku70 binding to HIV-1 integrase.

    abstract：BACKGROUND:HIV-1 integration results in genomic DNA gaps that are repaired by cellular DNA repair pathways. This step of the lentiviral life cycle remains poorly understood despite its crucial importance for successful replication. We and others reported that Ku70 protein of the non-homologous end joining pathway (NHEJ...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-019-0492-z

    authors： Knyazhanskaya E,Anisenko A,Shadrina O,Kalinina A,Zatsepin T,Zalevsky A,Mazurov D,Gottikh M

    更新日期：2019-11-06 00:00:00

  • HIV-1 capsid is involved in post-nuclear entry steps.

    abstract：BACKGROUND:HIV-1 capsid influences viral uncoating and nuclear import. Some capsid is detected in the nucleus but it is unclear if it has any function. We reported that the antibiotic Coumermycin-A1 (C-A1) inhibits HIV-1 integration and that a capsid mutation confers resistance to C-A1, suggesting that capsid might aff...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-016-0262-0

    authors： Chen NY,Zhou L,Gane PJ,Opp S,Ball NJ,Nicastro G,Zufferey M,Buffone C,Luban J,Selwood D,Diaz-Griffero F,Taylor I,Fassati A

    更新日期：2016-04-23 00:00:00

  • Proteasome inhibitors act as bifunctional antagonists of human immunodeficiency virus type 1 latency and replication.

    abstract：BACKGROUND:Existing highly active antiretroviral therapy (HAART) effectively controls viral replication in human immunodeficiency virus type 1 (HIV-1) infected individuals but cannot completely eradicate the infection, at least in part due to the persistence of latently infected cells. One strategy that is being active...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-10-120

    authors： Miller LK,Kobayashi Y,Chen CC,Russnak TA,Ron Y,Dougherty JP

    更新日期：2013-10-24 00:00:00

  • The S40 residue in HIV-1 Gag p6 impacts local and distal budding determinants, revealing additional late domain activities.

    abstract：BACKGROUND:HIV-1 budding is directed primarily by two motifs in Gag p6 designated as late domain-1 and -2 that recruit ESCRT machinery by binding Tsg101 and Alix, respectively, and by poorly characterized determinants in the capsid (CA) domain. Here, we report that a conserved Gag p6 residue, S40, impacts budding media...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-10-143

    authors： Watanabe SM,Chen MH,Khan M,Ehrlich L,Kemal KS,Weiser B,Shi B,Chen C,Powell M,Anastos K,Burger H,Carter CA

    更新日期：2013-11-21 00:00:00

  • HIV-1 envelope replication and α4β7 utilization among newly infected subjects and their corresponding heterosexual partners.

    abstract：BACKGROUND:Previous studies suggest that active selection limits the number of HIV-1 variants acquired by a newly infected individual from the diverse variants circulating in the transmitting partner. We compared HIV-1 envelopes from 9 newly infected subjects and their linked transmitting partner to explore potential m...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-10-162

    authors： Pena-Cruz V,Etemad B,Chatziandreou N,Nyein PH,Stock S,Reynolds SJ,Laeyendecker O,Gray RH,Serwadda D,Lee SJ,Quinn TC,Sagar M

    更新日期：2013-12-26 00:00:00

  • Discovery of a diaminoquinoxaline benzenesulfonamide antagonist of HIV-1 Nef function using a yeast-based phenotypic screen.

    abstract：BACKGROUND:HIV-1 Nef is a viral accessory protein critical for AIDS progression. Nef lacks intrinsic catalytic activity and binds multiple host cell signaling proteins, including Hck and other Src-family tyrosine kinases. Nef binding induces constitutive Hck activation that may contribute to HIV pathogenesis by promoti...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-10-135

    authors： Trible RP,Narute P,Emert-Sedlak LA,Alvarado JJ,Atkins K,Thomas L,Kodama T,Yanamala N,Korotchenko V,Day BW,Thomas G,Smithgall TE

    更新日期：2013-11-14 00:00:00

  • Higher levels of Zidovudine resistant HIV in the colon compared to blood and other gastrointestinal compartments in HIV infection.

    abstract：BACKGROUND:The gut-associated lymphoid tissue (GALT) is the largest lymphoid organ infected by human immunodeficiency virus type 1 (HIV-1). It serves as a viral reservoir and host-pathogen interface in infection. This study examined whether different parts of the gut and peripheral blood lymphocytes (PBL) contain diffe...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-7-74

    authors： van Marle G,Church DL,Nunweiler KD,Cannon K,Wainberg MA,Gill MJ

    更新日期：2010-09-13 00:00:00

  • Physiological properties of astroglial cell lines derived from mice with high (SAMP8) and low (SAMR1, ICR) levels of endogenous retrovirus.

    abstract：:Previous studies have reported that various inbred SAM mouse strains differ markedly with regard to a variety of parameters, such as capacity for learning and memory, life spans and brain histopathology. A potential cause of differences seen in these strains may be based on the fact that some strains have a high conce...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-5-104

    authors： Kim BH,Meeker HC,Shin HY,Kim JI,Jeong BH,Choi EK,Carp RI,Kim YS

    更新日期：2008-11-25 00:00:00

  • Human TRIM5alpha mediated restriction of different HIV-1 subtypes and Lv2 sensitive and insensitive HIV-2 variants.

    abstract：:In order to characterize the antiviral activity of human TRIM5alpha in more detail human derived indicator cell lines over expressing wild type human TRIM5alpha were generated and challenged with HIV-1 and HIV-2 viruses pseudotyped with HIV envelope proteins in comparison to VSV-G pseudotyped particles. HIV envelope p...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-3-79

    authors： Kaumanns P,Hagmann I,Dittmar MT

    更新日期：2006-11-06 00:00:00

  • The distribution of insertionally polymorphic endogenous retroviruses in breast cancer patients and cancer-free controls.

    abstract：BACKGROUND:Integration of retroviral DNA into a germ cell can result in a provirus that is transmitted vertically to the host's offspring. In humans, such endogenous retroviruses (HERVs) comprise >8% of the genome. The HERV-K(HML-2) proviruses consist of ~90 elements related to mouse mammary tumor virus, which causes b...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-014-0062-3

    authors： Wildschutte JH,Ram D,Subramanian R,Stevens VL,Coffin JM

    更新日期：2014-08-12 00:00:00

  • Single genome analysis reveals genetic characteristics of Neuroadaptation across HIV-1 envelope.

    abstract：BACKGROUND:The widespread use of highly effective, combination antiretroviral therapy (cART) has led to a significant reduction in the incidence of HIV-associated dementia (HAD). Despite these advances, the prevalence of HIV-1 associated neurocognitive disorders (HANDs) has been estimated at approximately 40%-50%. In t...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-014-0065-0

    authors： Evering TH,Kamau E,St Bernard L,Farmer CB,Kong XP,Markowitz M

    更新日期：2014-08-15 00:00:00

  • Interferon regulatory factor 4 as a therapeutic target in adult T-cell leukemia lymphoma.

    abstract：BACKGROUND:Adult T-cell leukemia lymphoma (ATLL) is a chemotherapy-resistant malignancy with a median survival of less than one year that will afflict between one hundred thousand and one million individuals worldwide who are currently infected with human T-cell leukemia virus type 1. Recurrent somatic mutations in hos...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-020-00535-z

    authors： Rauch DA,Olson SL,Harding JC,Sundaramoorthi H,Kim Y,Zhou T,MacLeod AR,Challen G,Ratner L

    更新日期：2020-08-28 00:00:00

  • Moving beyond the mousetrap: current and emerging humanized mouse and rat models for investigating prevention and cure strategies against HIV infection and associated pathologies.

    abstract：:The development of safe and effective combination antiretroviral therapies for human immunodeficiency virus (HIV) infection over the past several decades has significantly reduced HIV-associated morbidity and mortality. Additionally, antiretroviral drugs have provided an effective means of protection against HIV trans...

    journal_title：Retrovirology

    pub_type： 杂志文章,评审

    doi：10.1186/s12977-020-00515-3

    authors： Agarwal Y,Beatty C,Biradar S,Castronova I,Ho S,Melody K,Bility MT

    更新日期：2020-04-10 00:00:00

  • Tenofovir treatment augments anti-viral immunity against drug-resistant SIV challenge in chronically infected rhesus macaques.

    abstract：BACKGROUND:Emergence of drug-resistant strains of human immunodeficiency virus type 1 (HIV-1) is a major obstacle to successful antiretroviral therapy (ART) in HIV-infected patients. Whether antiviral immunity can augment ART by suppressing replication of drug-resistant HIV-1 in humans is not well understood, but can b...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-3-97

    authors： Metzner KJ,Binley JM,Gettie A,Marx P,Nixon DF,Connor RI

    更新日期：2006-12-21 00:00:00

  • MDM2 is a novel E3 ligase for HIV-1 Vif.

    abstract：:The human immunodeficiency virus type 1 (HIV-1) Vif plays a crucial role in the viral life cycle by antagonizing a host restriction factor APOBEC3G (A3G). Vif interacts with A3G and induces its polyubiquitination and subsequent degradation via the formation of active ubiquitin ligase (E3) complex with Cullin5-ElonginB...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-6-1

    authors： Izumi T,Takaori-Kondo A,Shirakawa K,Higashitsuji H,Itoh K,Io K,Matsui M,Iwai K,Kondoh H,Sato T,Tomonaga M,Ikeda S,Akari H,Koyanagi Y,Fujita J,Uchiyama T

    更新日期：2009-01-07 00:00:00

  • Conference highlights of the 16th International Conference on Human Retrovirology: HTLV and related retroviruses, 26-30 June 2013, Montreal, Canada.

    abstract：:The 16th International Conference on Human Retrovirology: HTLV and Related Retroviruses was held in Montreal, Québec from June 26th to June 30th, 2013 and was therefore hosted by a Canadian city for the first time. The major topic of the meeting was human T-lymphotropic viruses (HTLVs) and was covered through distinct...

    journal_title：Retrovirology

    pub_type：

    doi：10.1186/1742-4690-11-19

    authors： Barbeau B,Hiscott J,Bazarbachi A,Carvalho E,Jones K,Martin F,Matsuoka M,Murphy EL,Ratner L,Switzer WM,Watanabe T

    更新日期：2014-02-24 00:00:00

  • Natural APOBEC3C variants can elicit differential HIV-1 restriction activity.

    abstract：BACKGROUND:The APOBEC3 (A3) family of DNA cytosine deaminases provides an innate barrier to infection by retroviruses including HIV-1. A total of five enzymes, A3C, A3D, A3F, A3G and A3H, are degraded by the viral accessory protein Vif and expressed at high levels in CD4+ T cells, the primary reservoir for HIV-1 replic...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-018-0459-5

    authors： Anderson BD,Ikeda T,Moghadasi SA,Martin AS,Brown WL,Harris RS

    更新日期：2018-12-17 00:00:00

  • Longitudinal analysis of subtype C envelope tropism for memory CD4+ T cell subsets over the first 3 years of untreated HIV-1 infection.

    abstract：BACKGROUND:HIV-1 infects a wide range of CD4+ T cells with different phenotypic properties and differing expression levels of entry coreceptors. We sought to determine the viral tropism of subtype C (C-HIV) Envelope (Env) clones for different CD4+ T cell subsets and whether tropism changes during acute to chronic disea...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-020-00532-2

    authors： Gartner MJ,Gorry PR,Tumpach C,Zhou J,Dantanarayana A,Chang JJ,Angelovich TA,Ellenberg P,Laumaea AE,Nonyane M,Moore PL,Lewin SR,Churchill MJ,Flynn JK,Roche M

    更新日期：2020-08-06 00:00:00

  • The XVI International Conference on AIDS: the place to be!

    abstract：:This editorial represents a plea to retrovirologists to attend the XVI International Conference on AIDS that will take place in Toronto, Canada between August 13-18, 2006. In short, it is vital that politicians and opinion leaders understand that basic scientists are no less committed to the worldwide battle against H...

    journal_title：Retrovirology

    pub_type： 社论

    doi：10.1186/1742-4690-3-9

    authors： Wainberg MA

    更新日期：2006-02-03 00:00:00

  • A purine-rich element in foamy virus pol regulates env splicing and gag/pol expression.

    abstract：BACKGROUND:The foamy viral genome encodes four central purine-rich elements localized in the integrase-coding region of pol. Previously, we have shown that the first two of these RNA elements (A and B) are required for protease dimerization and activation. The D element functions as internal polypurine tract during rev...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-017-0337-6

    authors： Moschall R,Denk S,Erkelenz S,Schenk C,Schaal H,Bodem J

    更新日期：2017-02-06 00:00:00

  • Virus-producing cells determine the host protein profiles of HIV-1 virion cores.

    abstract：BACKGROUND:Upon HIV entry into target cells, viral cores are released and rearranged into reverse transcription complexes (RTCs), which support reverse transcription and also protect and transport viral cDNA to the site of integration. RTCs are composed of viral and cellular proteins that originate from both target and...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-9-65

    authors： Santos S,Obukhov Y,Nekhai S,Bukrinsky M,Iordanskiy S

    更新日期：2012-08-13 00:00:00

  • Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses.

    abstract：:Efforts to assess the prevalence of xenotropic murine leukemia virus-related virus (XMRV) in patients with prostate cancer and chronic fatigue syndrome have relied heavily on PCR-based testing of clinical samples and have yielded widely divergent findings. This week in Retrovirology, reports from four independent rese...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-7-112

    authors： Smith RA

    更新日期：2010-12-20 00:00:00

  • SIVdrl detection in captive mandrills: are mandrills infected with a third strain of simian immunodeficiency virus?

    abstract：:A pol-fragment of simian immunodeficiency virus (SIV) that is highly related to SIVdrl-pol from drill monkeys (Mandrillus leucophaeus) was detected in two mandrills (Mandrillus sphinx) from Amsterdam Zoo. These captivity-born mandrills had never been in contact with drill monkeys, and were unlikely to be hybrids. Thei...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-1-36

    authors： van der Kuyl AC,van den Burg R,Hoyer MJ,Gruters RA,Osterhaus AD,Berkhout B

    更新日期：2004-11-01 00:00:00

  • Socioeconomic status (SES) as a determinant of adherence to treatment in HIV infected patients: a systematic review of the literature.

    abstract：OBJECTIVES:It has been shown that socioeconomic status (SES) is associated with adherence to treatment of patients with several chronic diseases. However, there is a controversy regarding the impact of SES on adherence among patients with the human immunodeficiency virus (HIV) infection or acquired immunodeficiency syn...

    journal_title：Retrovirology

    pub_type： 社论,评审

    doi：10.1186/1742-4690-5-13

    authors： Falagas ME,Zarkadoulia EA,Pliatsika PA,Panos G

    更新日期：2008-02-01 00:00:00

  • Genome-wide association studies on HIV susceptibility, pathogenesis and pharmacogenomics.

    abstract：:Susceptibility to HIV-1 and the clinical course after infection show a substantial heterogeneity between individuals. Part of this variability can be attributed to host genetic variation. Initial candidate gene studies have revealed interesting host factors that influence HIV infection, replication and pathogenesis. R...

    journal_title：Retrovirology

    pub_type： 杂志文章,评审

    doi：10.1186/1742-4690-9-70

    authors： van Manen D,van 't Wout AB,Schuitemaker H

    更新日期：2012-08-24 00:00:00

  • Trypanosoma cruzi (Chagas' disease agent) reduces HIV-1 replication in human placenta.

    abstract：BACKGROUND:Several factors determine the risk of HIV mother-to-child transmission (MTCT), such as coinfections in placentas from HIV-1 positive mothers with other pathogens. Chagas' disease is one of the most endemic zoonoses in Latin America, caused by the protozoan Trypanosoma cruzi. The purpose of the study was to d...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-5-53

    authors： Dolcini GL,Solana ME,Andreani G,Celentano AM,Parodi LM,Donato AM,Elissondo N,González Cappa SM,Giavedoni LD,Martínez Peralta L

    更新日期：2008-07-01 00:00:00

  • Memories of John N. Brady: scientist, mentor and friend.

    abstract：:Friends and colleagues remember John N. Brady, Ph.D., Chief of the Virus Tumor Biology Section of the Laboratory of Cellular Oncology, who died much too young at the age of 57 on April 27, 2009 of colon cancer. John grew up in Illinois and received his Ph.D. with Dr. Richard Consigli at Kansas State University studyin...

    journal_title：Retrovirology

    pub_type： 传,社论,历史文章

    doi：10.1186/1742-4690-6-48

    authors： Pise-Masison CA,Marriott SJ

    更新日期：2009-05-19 00:00:00

  • HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain.

    abstract：BACKGROUND:The HIV-1 Rev regulatory protein binds as an oligomeric complex to viral RNA mediating nuclear export of incompletely spliced and non-spliced viral mRNAs encoding the viral structural proteins. However, the biological significance of the obligatory complex formation of Rev upon the viral RNA is unclear. RES...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-2-39

    authors： Furnes C,Arnesen T,Askjaer P,Kjems J,Szilvay AM

    更新日期：2005-06-10 00:00:00

  • Preferences for the selection of unique tRNA primers revealed from analysis of HIV-1 replication in peripheral blood mononuclear cells.

    abstract：BACKGROUND:All human immunodeficiency virus (HIV-1) uses a host tRNALys,3 as the primer for reverse transcription. The tRNALys,3 is bound to a region on the HIV-1 genome, the primer-binding site (PBS), that is complementary to the 18 terminal nucleotides of tRNALys,3. How HIV-1 selects the tRNA from the intracellular m...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-2-21

    authors： Moore-Rigdon KL,Kosloff BR,Kirkman RL,Morrow CD

    更新日期：2005-03-24 00:00:00