Abstract:
:The objective of this study was to create and evaluate a routine (macro) using Image-Pro Plus 4.5 software (Media Cybernetics, Silver Spring, USA) for automatic counting of labeled nuclei by proliferating cell nuclear antigen (PCNA) immunohistochemistry. A total of 154 digital color images were obtained from eleven sections of reticular oral lichen planus stained by PCNA immunohistochemistry. Mean density (gray-level), red density, green density, blue density, area, minor axis, perimeter rate and roundness were parameters used for PCNA labeled nuclei discrimination, followed by their outlined presentation and counting in each image by the macro. Mean density and area thresholds were automatically defined based, respectively, on mean density and mean area of PCNA labeled nuclei in the assessed image. The reference method consisted in visual counting of manually outlined labeled nuclei. Statistical analysis of macro results versus reference countings showed a very significant correlation (rs = 0.964, p < 0.001) for general results and a high level (89.8 +/- 3.8%) of correctly counted labeled nuclei. We conclude that the main parameters associated with a high correlation between macro and reference results were mean density (gray-level) and area thresholds based on image profiles; and that Image-Pro Plus 4.5 using a routine with automatic definition of mean density and area thresholds can be considered a valid alternative to visual counting of PCNA labeled nuclei.
journal_name
Braz Oral Resjournal_title
Brazilian oral researchauthors
Francisco JS,Moraes HP,Dias EPdoi
10.1590/s1806-83242004000200002subject
Has Abstractpub_date
2004-04-01 00:00:00pages
100-4issue
2eissn
1806-8324issn
1807-3107pii
S1517-74912004000200002journal_volume
18pub_type
杂志文章abstract::The wide use of dental bleaching treatment has brought concern about the possible effects of hydrogen peroxide on dental tissue and restorative materials. The objective of this study was to evaluate in vitro the effect of nightguard bleaching on the surface roughness of dental ceramics after different periods of bleac...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242011000500013
更新日期:2011-09-01 00:00:00
abstract::This study assessed the cross-sectional Knoop microhardness and flexural strength of root dentin exposed to different surface treatments with chemical agents after biomechanical preparation. Root canals from human canines were biomechanically treated and divided into eight groups (n=10) to receive one of the following...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2014.vol28.0052
更新日期:2014-01-01 00:00:00
abstract::Questionnaires for the assessment of knowledge and self-perception can be useful to diagnose what a dentist knows about management and administration. The aim of the present study was to create and validate the Dental Management Survey Brazil (DMS-BR) scale, based on meetings with experts in the field. After having el...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107BOR-2017.vol31.0026
更新日期:2017-04-10 00:00:00
abstract::The sense of coherence (SOC) is a measure of global orientation regarding the ability of individuals to cope with stressful situations. The aim of the present study was to evaluate the association between SOC and quality of life (QoL) and clinical and sociodemographic characteristics among survivors of oral, oropharyn...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2020.vol34.0009
更新日期:2020-02-07 00:00:00
abstract::The objective of this study was to assess the prevalence and the type of claim denials (administrative, clinical or both) made by a large dental insurance plan. This was a cross-sectional, observational study, which retrospectively collected data from the claims and denial reports of a dental insurance company. The sa...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107BOR-2015.vol29.0041
更新日期:2015-01-01 00:00:00
abstract::To evaluate the association between temporomandibular disorder (TMD) and anxiety, quality of sleep, and quality of life in nursing professionals at the Hospital de Clínicas de Uberlândia of the Universidade Federal de Uberlandia--HCU-UFU (Medical University Hospital of the Federal University of Uberlândia), four quest...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107BOR-2015.vol29.0070
更新日期:2015-01-01 00:00:00
abstract::To assess the impact of oral conditions on the oral health-related quality of life (OHRQoL) of pediatric liver transplant candidates. This cross-sectional study included 60 children aged 13 to 48 months who were liver transplant candidates that attended the AC Camargo Cancer Center, São Paulo, Brazil. On the day of or...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2020.vol34.0100
更新日期:2020-01-01 00:00:00
abstract::The aim of this study was to detect possible differences in the EMG (electromyography) activity, chewing rate (CR), cycle duration (CD) and preferred chewing side (PCS) between children with and without unilateral posterior crossbite. Thirty-seven children aged from 7 to 10 years were selected from the clinic of the D...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242010000200013
更新日期:2010-04-01 00:00:00
abstract::The aim of this study was to investigate the prevalence of oral and maxillofacial lesions among children from representative regions of Brazil. A retrospective descriptive study was conducted. Biopsy records comprising the period from 2000 to 2015 were obtained from the archives of three Brazilian oral pathology refer...
journal_title:Brazilian oral research
pub_type: 杂志文章,多中心研究
doi:10.1590/1807-3107bor-2018.vol32.0020
更新日期:2018-03-15 00:00:00
abstract::The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimester of pregnancy and ...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242012000500011
更新日期:2012-09-01 00:00:00
abstract::The lack of guidelines for bone augmentation procedures might compromise decision making in implantology. The objective of this study was to perform a retrospective study to verify the outcomes of horizontal bone reconstruction in implant dentistry with different types of materials and amounts of native bone in the re...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2018.vol32.0021
更新日期:2018-01-01 00:00:00
abstract::This epidemiologic survey aimed at assessing the prevalence of traumatic dental injuries in children seen at the Federal University of Rio de Janeiro, Brazil. The records of a total of 111 children (aged 0 to 6 years) seen from 2004 to 2006 in the dental trauma clinic were surveyed, comprising a total of 201 traumatiz...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242010000100015
更新日期:2010-01-01 00:00:00
abstract::This study has defined the cephalometric values of the Craniofacial Analysis of the Tweed Foundation for a sample of Brazilian subjects. The sample consisted of 211 cephalometric radiographs from subjects aged 12-15, which were divided into two groups: Class II group, with 168 lateral teleradiographs (cephalograms) of...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242005000100013
更新日期:2005-01-01 00:00:00
abstract::This study evaluated the effects of 10% alphatocopherol on the fracture resistance of endodontically treated teeth subjected to tooth bleaching with hydrogen peroxide and immediately restored with composite resin. Fifty bovine incisors were selected, including 10 sound teeth that constituted the control group (G1 (C))...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107BOR-2016.vol30.0069
更新日期:2016-05-20 00:00:00
abstract::The present study evaluated the antimicrobial activity of three root canal irrigants against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus. These microorganisms were incubated in the presence of citric acid (6 and 10%), EDTA (17%), and NaOCl (0.5, 1.0, 2.5, and 5.25%). Agar diffusion tests were pe...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242010000400006
更新日期:2010-10-01 00:00:00
abstract::The objective of this study was to evaluate the expression of matrix metalloproteinase 9 (MMP-9) and transforming growth factor beta (TGF-β1) in periapical lesion samples correlated with the intensity of the inflammatory infiltrate and thickness of the epithelial lining. Forty-five cases of periapical lesions (23 peri...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107BOR-2017.vol31.0051
更新日期:2017-07-03 00:00:00
abstract::The aim of this study was to determine if multiple processing (heat-pressing) of a dental ceramic influences flexural strength, hardness, and microstructure. Ninety bar-shaped specimens (15 mm × 4 mm × 2 mm) of a pressed ceramic (Vita PM9) were fabricated and randomly divided into 6 groups (n = 15) according to the fa...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2018.vol32.0106
更新日期:2018-10-25 00:00:00
abstract::Satisfaction with dental aesthetics is a subjective indicator used in epidemiological studies and is related to health behaviours. Little is known about the factors that influence this indicator, particularly among adolescents who live in a situation of social vulnerability. The aim of this study was to investigate th...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2019.vol33.0014
更新日期:2019-03-18 00:00:00
abstract::This study aimed to analyze the 100 most-cited papers in the field of oral medicine and pathology over time, identifying the areas of more intense research. Papers in journals of oral medicine and pathology were identified using the Web of Science database. The specified research period was between 1900 and 2019. Desc...
journal_title:Brazilian oral research
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更新日期:2020-12-18 00:00:00
abstract::This study investigated the effect of coinitiator solutions and self-adhesive resin cement on the early retention of glass-fiber posts. Cylindrical glass-fiber posts were luted into 40 incisor roots with different adhesion strategies (n = 10): SB2, Single Bond 2 + conventional resin cement (RelyX ARC); AP, Scotchbond ...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242012005000017
更新日期:2012-09-01 00:00:00
abstract::The present systematic review was performed to investigate if there is evidence justifying the prophylactic extraction of third molars, one of the most frequent procedures in oral surgery. A series of searches was carried out for randomized, clinical trials and systematic reviews in seven databases (MEDLINE, BBO, LILA...
journal_title:Brazilian oral research
pub_type: 杂志文章,评审
doi:
更新日期:2013-03-01 00:00:00
abstract::Recent studies investigating protease-activated receptor type 2 (PAR-2) suggest an association between the receptor and periodontal inflammation. It is known that gingipain, a bacterial protease secreted by the important periodontopathogen Porphyromonas gingivalis can activate PAR-2. Previous studies by our group foun...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107BOR-2017.vol31.0016
更新日期:2017-01-26 00:00:00
abstract::The purpose of this study was to evaluate whether the use of desensitizing dentifrices used 15 days prior to and after in-office tooth bleaching could eliminate or reduce tooth sensitivity. After institutional review board approval and informed consent, 45 subjects were selected and divided into 3 groups according to ...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/S1806-83242013000600012
更新日期:2013-11-01 00:00:00
abstract::To estimate the prevalence and related aspects of periodontitis in a rural area of the State of Bahia, Brazil, this cross-sectional study was carried out in the village of Matinha dos Pretos, Feira de Santana County, Bahia, among 172 subjects ranging from 20 to 60 years of age. During household visits, a full-mouth pe...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242006000300014
更新日期:2006-07-01 00:00:00
abstract::The aim of this study was to verify the prevalence of signs and symptoms of temporomandibular disorders (TMD) in adolescents and its relationship to gender. The sample comprised 217 subjects, aged 12 to 18. The subjective symptoms and clinical signs of TMD were evaluated, using, respectively, a self-report questionnai...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242005000200004
更新日期:2005-04-01 00:00:00
abstract::Human Papilomaviruses (HPV) are a group of viruses associated with benign and malignant lesions of cutaneous and mucosal epithelia. Some "high risk" HPV types, especially HPV 16 and 18, are strongly correlated with cervical and anogenital cancers and are also related to the genesis of oral squamous cell carcinomas (OS...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242006000100005
更新日期:2006-01-01 00:00:00
abstract::The aim of this study was to establish two new formulas for objectively evaluating skeletal maturation of cervical vertebrae in female and male Brazilian subjects using lateral cephalometric radiographs. The sample included 128 girls and 110 boys, aged 7.0 to 15.9 years, from the files of the Oral Radiology Clinic, Pi...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242007000400009
更新日期:2007-10-01 00:00:00
abstract::Evidence shows the polymicrobial etiology of endodontic infections, in which bacteria and their products are the main agents for the development, progression, and dissemination of apical periodontitis. Microbial factors in necrotic root canals (e.g., endotoxin) may spread into apical tissue, evoking and supporting a c...
journal_title:Brazilian oral research
pub_type: 杂志文章,评审
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更新日期:2018-10-18 00:00:00
abstract::The aims of the present study were to compare conventional radiography, radiographs digitized with a scanner or photographic camera, and digital radiography, used to evaluate the radiopacity of endodontic materials, and to compare the accuracy of linear and quadratic models used to convert radiopacity values to equiva...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2020.vol34.0080
更新日期:2020-01-01 00:00:00
abstract::This study analyzed the effects of unilateral detachment of the temporal muscle and coronoidotomy on facial growth in young rats. Thirty one-month-old Wistar rats were distributed into three groups: detachment, coronoidotomy and sham-operated. Under general anesthesia, unilateral detachment of the temporal muscle was ...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242012000400011
更新日期:2012-07-01 00:00:00