Abstract:
:Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/μL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex® backing via an ARclean® double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets.
journal_name
Biosens Bioelectron Xjournal_title
Biosensors & bioelectronics: Xauthors
Davidson JL,Wang J,Maruthamuthu MK,Dextre A,Pascual-Garrigos A,Mohan S,Putikam SVS,Osman FOI,McChesney D,Seville J,Verma MSdoi
10.1016/j.biosx.2021.100076keywords:
["Colorimetric LAMP","Microfluidic paper-based analytical devices","Paper-based diagnostics","SARS-CoV-2","Saliva"]subject
Has Abstractpub_date
2021-12-01 00:00:00pages
100076issn
2590-1370pii
S2590-1370(21)00012-1journal_volume
9pub_type
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