Abstract:
OBJECTIVES:Synthetic biology has emerged from molecular biology and engineering approaches and aims to develop novel, biologically-inspired systems for industrial and basic research applications ranging from biocomputing to drug production. Surprisingly, redoxin (thioredoxin, glutaredoxin, peroxiredoxin) and other thiol-based redox systems have not been widely utilized in many of these synthetic biology applications. METHODS:We reviewed thiol-based redox systems and the development of synthetic biology applications that have used thiol-dependent parts. RESULTS:The development of circuits to facilitate cytoplasmic disulfide bonding, biocomputing and the treatment of intestinal bowel disease are amongst the applications that have used thiol-based parts. We propose that genetically encoded redox sensors, thiol-based biomaterials and intracellular hydrogen peroxide generators may also be valuable components for synthetic biology applications. DISCUSSION:Thiol-based systems play multiple roles in cellular redox metabolism, antioxidant defense and signaling and could therefore offer a vast and diverse portfolio of components, parts and devices for synthetic biology applications. However, factors limiting the adoption of redoxin systems for synthetic biology applications include the orthogonality of thiol-based components, limitations in the methods to characterize thiol-based systems and an incomplete understanding of the design principles of these systems.
journal_name
Redox Repjournal_title
Redox report : communications in free radical researchauthors
Pillay CS,John Ndoi
10.1080/13510002.2021.1966183keywords:
["oxidative stress","peroxide","reactive oxygen species","redox sensors","redox signaling","redox systems biology","redoxin","synthetic biology"]subject
Has Abstractpub_date
2021-12-01 00:00:00pages
147-159issue
1eissn
1351-0002issn
1743-2928journal_volume
26pub_type
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