Endothelin-mediated remodeling in aortas of diabetic rats.


BACKGROUND:Smooth muscle cells proliferation and extracellular matrix (ECM) protein deposition are key features of diabetic macroangiopathy. In the present study, we have studied the role of endothelin(A) (ET(A)) receptor, the predominant receptor on smooth muscle cells, in diabetes-induced vascular hypertrophy and remodeling. METHODS:Streptozotocin-induced diabetic rats were administrated a selective ET(A) receptor antagonist, TBC3214, for 26 weeks. Following treatment, aortas were harvested and subjected to gene expression and morphometric analyses. We quantified fibronectin (FN) and plasminogen activator inhibitor-1 (PAI-1) expression as indicators of increased ECM protein synthesis. ET-1, ET-3, transforming growth factor-beta1 (TGF-beta1) and angiotensinogen mRNA levels were measured to elucidate genes involved in FN expression. We have investigated an embryonic splice variant of FN, oncofetal FN, and nonmuscle myosin heavy chain (SMemb) as vascular remodeling indicators. RESULTS:Our results show that diabetes leads to upregulation of FN, PAI-1, ET-1, ET-3, TGF-beta1 and angiotensinogen mRNA levels in association with increased medial thickness. Immunohistochemical analyses revealed concurrent protein level changes. Diabetes also upregulated oncofetal FN and SMemb mRNA levels. Treatment with TBC3214 attenuated the mRNA levels of several genes and prevented increased medial thickness. CONCLUSIONS:These results indicate that diabetes-induced vascular hypertrophy and remodeling is associated with reexpression of embryonic forms of FN and myosin heavy chain. Such changes are ET-dependent and may be mediated via TGF-beta1 and angiotensin.


Diabetes Metab Res Rev


Fukuda G,Khan ZA,Barbin YP,Farhangkhoee H,Tilton RG,Chakrabarti S





Has Abstract


2005-07-01 00:00:00












  • Prevalence and management of diabetic neuropathy in secondary care in Qatar.

    abstract:AIMS:Diabetic neuropathy (DN) is a "Cinderella" complication, particularly in the Middle East. A high prevalence of undiagnosed DN and those at risk of diabetic foot ulceration (DFU) is a major concern. We have determined the prevalence of DN and its risk factors, DFU, and those at risk of DFU in patients with type 2 d...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Ponirakis G,Elhadd T,Chinnaiyan S,Dabbous Z,Siddiqui M,Al-Muhannadi H,Petropoulos IN,Khan A,Ashawesh KAE,Dukhan KMO,Mahfoud ZR,Murgatroyd C,Slevin M,Malik RA

    更新日期:2020-05-01 00:00:00

  • Classification systems for lower extremity amputation prediction in subjects with active diabetic foot ulcer: a systematic review and meta-analysis.

    abstract:AIM:We systematically review the available systems used to classify diabetic foot ulcers in order to synthesize their methodological qualitative issues and accuracy to predict lower extremity amputation, as this may represent a critical point in these patients' care. MATERIAL AND METHODS:Two investigators searched, in...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,meta分析,评审


    authors: Monteiro-Soares M,Martins-Mendes D,Vaz-Carneiro A,Sampaio S,Dinis-Ribeiro M

    更新日期:2014-10-01 00:00:00

  • Diagnosis of infection in the foot in diabetes: a systematic review.

    abstract:BACKGROUND:Securing an early accurate diagnosis of diabetic foot infections and assessment of their severity are of paramount importance since these infections can cause great morbidity and potentially mortality and present formidable challenges in surgical and antimicrobial treatment. METHODS:In June 2018, we searche...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Senneville É,Lipsky BA,Abbas ZG,Aragón-Sánchez J,Diggle M,Embil JM,Kono S,Lavery LA,Malone M,van Asten SA,Urbančič-Rovan V,Peters EJG

    更新日期:2020-03-01 00:00:00

  • A linkage study of 12 IDDM susceptibility loci in the Finnish population.

    abstract:BACKGROUND:HLA region is the major locus (IDDM1) of type 1 diabetes (T1D) susceptibility. It explains approximately 50% of the genetic background of T1D, indicating additional genetic determinants. Genome scans and candidate gene studies have generated several chromosomal candidate regions that may have a role in T1D d...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 临床试验,杂志文章


    authors: Laine AP,Nejentsev S,Veijola R,Korpinen E,Sjöroos M,Simell O,Knip M,Akerblom HK,Ilonen J

    更新日期:2004-03-01 00:00:00

  • Outcomes of hyperglycemia in patients with and without diabetes hospitalized for infectious diseases.

    abstract:OBJECTIVE:To examine the prognostic implications of diabetes mellitus (DM) and the importance of glycemic control during hospitalization for infectious diseases. METHODS:Historical prospectively collected data of patients hospitalized between 2011 and 2013. Infection-related hospitalizations were classified according ...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Akirov A,Diker-Cohen T,Masri-Iraqi H,Duskin-Bitan H,Shimon I,Gorshtein A

    更新日期:2018-10-01 00:00:00

  • Cataract development in diabetic sand rats treated with alpha-lipoic acid and its gamma-linolenic acid conjugate.

    abstract:BACKGROUND:Diabetes commonly leads to long-term complications such as cataract. This study investigated the effects of alpha-lipoic acid (LPA) and its gamma-linolenic acid (GLA) conjugate on cataract development in diabetic sand rats. METHODS:Two separate experiments were conducted. In Experiment 1, sand rats were fed...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Borenshtein D,Ofri R,Werman M,Stark A,Tritschler HJ,Moeller W,Madar Z

    更新日期:2001-01-01 00:00:00

  • Essential phospholipids prevent islet damage induced by proinflammatory cytokines and hypoxic conditions.

    abstract:BACKGROUND:The pancreatic islet damage that occurs through an inflammatory response and hypoxia after infusion is a major hurdle in islet transplantation. Because essential phospholipids (EPL) have been shown to exhibit anti-inflammatory properties in liver disease, we analysed their protective effect on islets in infl...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Shahbazov R,Kanak MA,Takita M,Kunnathodi F,Khan O,Borenstein N,Lawrence MC,Levy MF,Naziruddin B

    更新日期:2016-03-01 00:00:00

  • Can molecular DNA-based techniques unravel the truth about diabetic foot infections?

    abstract::Diabetes foot infections are a common condition and a major causal pathway to lower extremity amputation. Identification of causative pathogens is vital in directing antimicrobial therapy. Historically, clinicians have relied upon culture-dependent techniques that are now acknowledged as both being selective for micro...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审


    authors: Malone M,Gosbell IB,Dickson HG,Vickery K,Espedido BA,Jensen SO

    更新日期:2017-01-01 00:00:00

  • Role of vasoactive factors in the pathogenesis of early changes in diabetic retinopathy.

    abstract::Several interactive and mutually perpetuating abnormal biochemical pathways, such as protein kinase C (PKC) activation, augmented polyol pathway, and non-enzymatic glycation, may be activated as a result of sustained hyperglycemia in diabetes. These abnormal pathways may in turn influence several vasoactive factors, w...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审


    authors: Chakrabarti S,Cukiernik M,Hileeto D,Evans T,Chen S

    更新日期:2000-11-01 00:00:00

  • Assessment of the relationship between hypoglycaemia awareness and autonomic function following islet cell/pancreas transplantation.

    abstract:BACKGROUND:This study assesses the autonomic function of patients who have regained awareness of hypoglycaemia following islet cell or whole pancreas transplant. METHODS:Five patients with type 1 diabetes and either islet cell (four patients) or whole pancreas (one patient) transplant were assessed. These patients wer...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Kamel JT,Goodman DJ,Howe K,Cook MJ,Ward GM,Roberts LJ

    更新日期:2015-09-01 00:00:00

  • Thymic expression of insulin-related genes in an animal model of autoimmune type 1 diabetes.

    abstract:BACKGROUND:Insulin and multiple other autoantigens have been implicated in the pathogenesis of autoimmune type 1 diabetes, but the origin of immunological self-reactivity specifically oriented against insulin-secreting islet beta-cells remains obscure. The primary objective of the present study was to investigate the h...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Kecha-Kamoun O,Achour I,Martens H,Collette J,Lefebvre PJ,Greiner DL,Geenen V

    更新日期:2001-03-01 00:00:00

  • Exercise training restores abnormal myocardial glucose utilization and cardiac function in diabetes.

    abstract:BACKGROUND:Clinical and experimental studies have shown that a reduction in myocardial glucose utilization is a factor contributing to diabetic cardiomyopathy. This study determined whether exercise training could prevent the depression in glucose utilization observed in the diabetic rat heart. METHODS:Diabetes was in...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Broderick TL,Poirier P,Gillis M

    更新日期:2005-01-01 00:00:00

  • The future for diabetic foot ulcer prevention: A paradigm shift from stratified healthcare towards personalized medicine.

    abstract::Prevention of diabetic foot ulcers is important to reduce the burden of diabetic foot disease. However, we found that ulcer prevention is underexposed in research and clinical practice. Barriers to explain this are seen in patient's goal-setting; in the lack of interdisciplinary teams for ulcer prevention; in sample s...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Van Netten JJ,Woodburn J,Bus SA

    更新日期:2020-03-01 00:00:00

  • The metabolic demands of driving for drivers with type 1 diabetes mellitus.

    abstract:BACKGROUND:The active cognitive-motor demands of driving may have a significant metabolic demand that could contribute to the development of hypoglycemia. Conversely, symptoms caused by the stress of driving may be confused with hypoglycemia and lead to false alarms. This study examined the metabolic demand and the phy...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Cox DJ,Gonder-Frederick LA,Kovatchev BP,Clarke WL

    更新日期:2002-09-01 00:00:00

  • Advanced glycation end products and diabetic foot disease.

    abstract::Diabetic foot disease is an important complication of diabetes. The development and outcome of foot ulcers are related to the interplay between numerous diabetes-related factors such as nerve dysfunction, impaired wound healing and microvascular and/or macrovascular disease.The formation of advanced glycation end prod...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审


    authors: Huijberts MS,Schaper NC,Schalkwijk CG

    更新日期:2008-05-01 00:00:00

  • Differences in cardiovascular risk profile based on relationship between post-load plasma glucose and fasting plasma levels.

    abstract:BACKGROUND:It has been shown that subjects with normal glucose tolerance (NGT), whose plasma glucose (PG) levels do not return to their fasting PG level within 2 h during an oral glucose tolerance test (OGTT) (Group I), have a significantly higher risk to develop type 2 diabetes than NGT subjects whose 2-h glucose retu...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Succurro E,Marini MA,Grembiale A,Lugarà M,Andreozzi F,Sciacqua A,Hribal ML,Lauro R,Perticone F,Sesti G

    更新日期:2009-05-01 00:00:00

  • Temporal trends in quality of primary care for patients with type 2 diabetes mellitus: A population-based retrospective cohort study after implementation of a quality improvement initiative.

    abstract:BACKGROUND:This study examined whether temporal trends exist in treatment of patients with type 2 diabetes (T2D) and quality of diabetes care after implementation of quality improvement initiative in primary care setting. METHODS:We conducted a population-based retrospective cohort study of 202,284 patients with T2D w...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Wong CKH,Fung CSC,Yu EYT,Wan EYF,Chan AKC,Lam CLK

    更新日期:2018-02-01 00:00:00

  • Maternal hypoglycemia during pregnancy in type 1 diabetes: maternal and fetal consequences.

    abstract::There is strong evidence that the avoidance of hyperglycemia is essential inoptimizing pregnancy outcome in type 1 diabetes. The price to pay is a striking increase in severe hypoglycemia (SH), defined as episodes requiring help from another person. During type 1 diabetic pregnancy, occurrence rates of SH up to 15 tim...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审


    authors: ter Braak EW,Evers IM,Willem Erkelens D,Visser GH

    更新日期:2002-03-01 00:00:00

  • Advanced glycosylation end products (AGEs), insulin-like growth factor-1 (IGF-1) and IGF-binding protein-3 (IGFBP-3) in patients with type 2 diabetes mellitus.

    abstract:BACKGROUND:Advanced glycosylation end product (AGE) formation is a major mechanism for the development of complications in diabetes, and the possible roles of insulin-like growth factor 1 (IGF-1) and IGF binding protein 3 (IGFBP-3) are not clearly established. METHODS:We examined the associations of AGEs, free IGF-I a...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Garay-Sevilla ME,Nava LE,Malacara JM,Wróbel K,Wróbel K,Pérez U

    更新日期:2000-03-01 00:00:00

  • Predicting ulcer-free survival using the discriminative value of screening test locations.

    abstract:BACKGROUND:Current guidelines recommend screening the feet of diabetic subjects with a 10-g monofilament or tuning fork. We investigated which tests and locations on the feet have the best predictive value regarding 1-year ulcer-free survival in diabetic subjects participating in the prospective Rotterdam Diabetic Foot...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Rinkel WD,van der Oest MJW,Dijkstra DA,Castro Cabezas M,Coert JH

    更新日期:2019-03-01 00:00:00

  • LIPS islet autoantibody assays in high-throughput format for DASP 2010.

    abstract:BACKGROUND:For cost-effective population-based diabetes prediction and confirmation, islet autoantibody assays must be made more economical. METHODS:We evaluated glutamic acid decarboxylase (GAD)-Ruc (renilla luciferase) and IA2ic (also known as ICA512ic)-Ruc (renilla luciferase) fusion protein constructs in high-thro...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Marcus P,Yan X,Bartley B,Hagopian W

    更新日期:2011-11-01 00:00:00

  • Intranasally administered insulin intended for prevention of type 1 diabetes--a safety study in healthy adults.

    abstract:BACKGROUND:Intranasally applied insulin is one of the antigen-specific therapies currently tested in clinical type 1 diabetes prevention trials, for example, in the Type 1 Diabetes Prediction and Prevention Study (DIPP). The possibility that the therapy may cause hypoglycaemia or local irritation and the poorly known i...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Kupila A,Sipilä J,Keskinen P,Simell T,Knip M,Pulkki K,Simell O

    更新日期:2003-09-01 00:00:00

  • Genetic association analyses of atopic illness and proinflammatory cytokine genes with type 1 diabetes.

    abstract:BACKGROUND:The genetic basis of the autoimmune disease type 1 diabetes (T1D) has now been largely determined, so now we can compare these findings with emerging genetic knowledge of disorders and phenotypes that have been negatively or positively associated with T1D historically. Here, we assessed the role in T1D of va...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Saleh NM,Raj SM,Smyth DJ,Wallace C,Howson JM,Bell L,Walker NM,Stevens HE,Todd JA

    更新日期:2011-11-01 00:00:00

  • Nocturnal hypoglycaemia in type 1 diabetes--consequences and assessment.

    abstract::Hypoglycaemia is inevitable when striving for low HbA1c values. Nocturnal hypoglycaemia often occurs without symptoms, but results in diminished next day well-being and hypoglycaemia unawareness. Frequency of nocturnal hypoglycaemia was first assessed in research ward settings, but suffered from insufficient glucose s...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审


    authors: DeVries JH,Wentholt IM,Masurel N,Mantel I,Poscia A,Maran A,Heine RJ

    更新日期:2004-11-01 00:00:00

  • Renin angiotensin system inhibitors may attenuate low LDL cholesterol-related cancer risk in type 2 diabetes.

    abstract:BACKGROUND:In type 2 diabetes (T2D), copresence of low-density lipoprotein cholesterol (LDL-C) < 2.8 mmol/L with triglyceride < 1.7 mmol/L or with albuminuria synergistically increased cancer risk. We tested whether use of renin angiotensin system inhibitors attenuated the increased cancer risk associated with these tw...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Yang X,Ma RC,So WY,Wang Y,Kong AP,Ozaki R,Xu G,Chan JC

    更新日期:2014-07-01 00:00:00

  • A practical approach to the clinical challenges in initiation of basal insulin therapy in people with type 2 diabetes.

    abstract::Initiating insulin therapy with a basal insulin analogue has become a standard of care in the treatment of type 2 diabetes mellitus (T2DM). Despite increasing choices in pharmacological approaches, intensified glucose monitoring and improvements in quality of care, many patients do not achieve the desired level of gly...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审


    authors: Forst T,Choudhary P,Schneider D,Linetzky B,Pozzilli P

    更新日期:2020-10-23 00:00:00

  • Self-monitoring of blood glucose in type-2 diabetes: what is the evidence?

    abstract:BACKGROUND:There is a controversy about self-monitoring of blood glucose (SMBG) in patients with type 2 diabetes who are not using insulin. Randomized trials are limited in duration, size, and validity. METHODS:Systematic search for randomized trials and observational studies published since 1990. For inclusion studie...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审


    authors: McGeoch G,Derry S,Moore RA

    更新日期:2007-09-01 00:00:00

  • The decrement in circulating endothelial progenitor cells (EPCs) in type 2 diabetes is independent of the severity of the hypoadiponectemia.

    abstract:BACKGROUND:Type 2 diabetes mellitus (DM) is associated with a decreased level of circulating endothelial progenitor cells (EPCs) and adiponectin. Experimental studies suggest a potential link between hypoadiponectinaemia and the depletion of the EPC level. This study investigated the relationships between adiponectin l...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: Li M,Ho JC,Lai KW,Au KK,Xu A,Cheung BM,Lam KS,Tse HF

    更新日期:2011-02-01 00:00:00

  • Effect of antiretroviral agents on carbohydrate metabolism in HIV-1 infected pregnant women.

    abstract:BACKGROUND:Despite the correlation between the use of protease inhibitors (PI) and adverse metabolic glycemic events, no prospective study has examined these parameters in pregnant women who use these drugs. METHODS:A prospective study was conducted on 57 pregnant women to investigate the effect of antiretroviral drug...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章


    authors: El Beitune P,Duarte G,Foss MC,Montenegro RM Jr,Spara P,Quintana SM,Figueiró-Filho EA,da Costa AG,Filho FM

    更新日期:2006-01-01 00:00:00

  • Should we change the target blood pressure in diabetic patients?

    abstract::Hypertension is a major modifiable risk factor for cardiovascular morbidity and mortality in diabetic patients. Guidelines recommend lowering blood pressure (BP) to less than 130/80 mmHg in diabetic patients. These recommendations are based on several studies in diabetic patients that showed the benefit of intensive B...

    journal_title:Diabetes/metabolism research and reviews

    pub_type: 杂志文章,评审


    authors: Shlomai G,Grossman E

    更新日期:2012-12-01 00:00:00